Single-molecule anisotropy imaging

Biophysical Journal

Volumn 77, Issue 5, 1999, Pages 2864-2870

  Harms, G S ^a   Sonnleitner, M ^a   Schutz G J ^a   Gruber, H J ^a   Schmidt, Thomas ^a,b  

^a JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; RHODAMINE;

ANISOTROPY; ARTICLE; CONTROLLED STUDY; DIFFUSION; FLUORESCENCE; IMAGING; MOLECULE; PHOSPHOLIPID MEMBRANE; PROTEIN CONFORMATION;

EID: 0032750790     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)77118-3     Document Type: Article

References (41)

1. Anderson, C. M., G. N. Georgiou, I. E. G. Monison, G. V. W. Stevenson, and R. J. Cherry. 1992. Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. J. Cell Sci. 101:415-425.
2. Aragon, S. R., and R. Percora. 1975. Fluorescence correlation spectroscopy as a probe of molecular dynamics. J. Chem. Phys. 64:1791-1803.
3. Axelrod, D. 1979. Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization. Biophys. J. 26: 557-574.
4. Axelrod, D., D. E. Koppel, J. Schlessinger, E. Elson, and W. W. Webb. 1976. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16:1055-1069.
5. Bernsdorff, C., A. Wolf, R. Winter, and E. Gratton. 1997. Effect of hydrostatic pressure on water penetration and rotational dynamics in phospholipid-cholesterol bilayers. Biophys. J. 72:1264-1277.
6. Bevington, P. R., and D. K. Robinson. 1992. Data Reduction and Error Analysis for the Physical Sciences. McGraw-Hill, New York.
7. Chandrasekhar, S. 1943. Stochastic problems in physics and astronomy. Rev. Mod. Phys. 15:1-80.
8. 2H and divalent cations on the motion in the phosphate region of the polar headgroup. Biochim. Biophys. Acta. 436:523-540.
9. Dickson, R. M., A. B. Cubitt, R. Y. Tsien, and W. E. Moerner. 1997. On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature. 388:355-358.
10. Dickson, R. M., D. J. Morris, and W. E. Moerner. 1998. Simultaneous imaging of individual molecules aligned both parallel and perpendicular to the optical axis. Phys. Rev. Lett. 24:5322-5325.
11. Edidin, M. 1987. Rotational and lateral diffusion of membrane proteins and lipids: phenomena and function. Curr. Top. Membr. Transp. 29:91-127.
12. Eigen, M., and R. Rigler. 1994. Sorting single molecules: application to diagnostics and evolutionary biotechnology. Proc. Natl. Acad. Sci. USA. 91:5740-5747.
13. Gelles, J., B. J. Schnapp, and M. P. Sheetz. 1988. Tracking kinesin-driven movements with nanometre-scale precision. Nature. 331:450-453.
14. Geerts, H., M. deBrabanter, R. Nuydens, S. Geuens, M. Moeremans, J. deMey, and P. Hollenbeck. 1987. Nanovid tracking: a new automatic method for the study of mobility in living cells based on colloidal gold and video microscopy. Biophys. J. 52:775-782.
15. 2H NMR studies of structure and motion in bilayers of phosphatidylcholine and phosphatidylethanolamine. Biochemistry. 27:7750-7758.
16. Gosh, R. N., and W. W. Webb. 1994. Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules. Biophys. J. 66:1301-1318.
17. Ha, T., Th. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss. 1996. Single molecule dynamics studied by polarization modulation. Phys. Rev. Lett. 77:3979-3982.
18. Ha, T., J. Glass, Th. Enderle, D. S. Chemla, and S. Weiss. 1998. Hindered rotational diffusion and jumps of single molecules. Phys. Rev. Lett. 80:2093-2096.
19. Kada, G. 1998. The quantitative determination of avidin and streptavidin with a new biotin-fluorescein conjugate. Diploma Thesis, University of Linz.
20. Kalb, E., S. Frey, and L. K. Tamm. 1992. Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers. Biochim. Biophys. Ada. 1103:307-316.
21. Lee, G. M., A. Ishihara, and K. A. Jacobson. 1991. Direct observation of brownian motion of lipids in a membrane. Proc. Natl. Acad. Sci. USA. 88:6274-6278.
22. Lieberherr, M., Ch. Fattinger, and W. Lukosz. 1987. Optical-environment-dependent effects on the fluorescence of submonomolecular dye layers on interfaces. Surf. Sci. 189:954-959.
23. MacKay, A. L. 1981. A proton NMR moment study of the gel and liquid-crystalline phases of dipalmitoyl phosphatidylcholine. Biophys. J. 35:301-313.
24. Quian, H., M. P. Sheetz, and E. L. Elson. 1991. Single particle tracking: analysis of diffusion and flow in two-dimensional systems. Biophys. J. 60:910-921.
25. Saffman, P. G., and M. Delbrück. 1975. Brownian motion in biological membranes. Proc. Natl. Acad. Sci. USA. 72:3111-3113.
26. Schaffer, J., A. Volkmer, C. Eggeling, V. Subramaniam, G. Striker, and C. A. M. Seidel. 1999. Identification of single molecules by timeresolved fluorescence anisotropy. J. Phys. Chem. 103:331-336.
27. Schmidt, Th., G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler. 1995. Pholophysics and motion of single fluorescent molecules in phospholipid membranes. J. Phys. Chem. 99:17662-17668.
28. Schmidt, Th., G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler. 1996. Imaging of single molecule diffusion. Proc. Natl. Acad. Sci. USA. 91:2926-2929.
29. Schütz, G. J., H. Schindler, and Th. Schmidt. 1997a. Single molecule dichroism. Opt. Lett. 22:561-564.
30. Schutz, G. J., H. Schindler, and Th. Schmidt. 1997b. Single-molecule microscopy on model membranes reveals anomalous diffusion. Biophys. J. 73:1073-1080.
31. Seelig, J. 1977. Deuterium magnetic resonance: theory and application to lipid membranes. Q. Rev. Biophys. 10:353-418.
32. Shin, Y.-K., U. Ewert, D. E. Budil, and J. H. Freed. 1991. Microscopic versus macroscopic diffusion in model membranes by electron spin resonance spectral-spatial imaging. Biophys. J. 59:950-957.
33. Shin, Y. K., and J. H. Freed. 1989. Dynamic imaging of lateral diffusion by electron spin resonance and study of rotational dynamics in model membanes. 55:537-550.
34. Soper, S. A., H. L. Nutter, R. A. Keller, D. M. Lloyd, and E. B. Shera. 1993. The photophysical constants of several fluorescent dyes pertaining to ultrasensitive fluorescence spectroscopy. Photochem. Photobiol. 57: 972-977.
35. Tamm, L. K., and E. Kalb. 1992. Microspectrofluorometry on supported planar membranes. In Molecular Luminescence Spectroscopy: Methods and Applications, Part 3. S. G. Schulman, editor. Wiley, New York.
36. Timbs, M. M., and N. L. Thompson. 1990. Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recovery. Biophys. J. 58:413-428.
37. Timbs, M. M, and N. L. Thompson. 1993. Measurement of restricted rotational diffusion of fluorescence lipids in supported planar phospholipid monolayers using angle-dependent polarized fluorescence photobleaching recovery. Biopolymers. 33:45-57.
38. Vaz, W. L. C., and P. F. Almeida. 1991, Microscopic versus macroscopic diffusion in one-component fluid phase lipid bilayer membranes. Biophys. J. 60:1553-1554.
39. Vaz, M. J., N. A. P. Vaz, W. Doane, and P. W. Westerman. 1979. Orientational order and rotational diffusion of the head group in the bilayer membrane. Biophys. J. 28:327-338.
40. Velez, M., and D. Axelrod. 1988. Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranes. Biophys. J. 53:575-591.
41. Widengren, J., Ü. Mets, and R. Rigler. 1995. Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study. J. Phys. Chem. 99:13368-13379.