Imaging and time-resolved spectroscopy of single molecules at an interface

Science

Volumn 272, Issue 5259, 1996, Pages 255-258

  Macklin, J J ^a   Trautman, J K ^a   Harris, T D ^a   Brus, L E ^a  

^a LUCENT TECHNOLOGIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000793711     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5259.255     Document Type: Article

References (30)

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14. 3 counts and was subtracted out of the data.
15. 2 at 532 nm, estimated by us on the basis of the measured absorption in methanol.
16. 12C(12) in toluene. The nanomolar dye solution was freshly prepared from a micromolar dye solution with each sample
17. -2, in a separate study we found that the saturation of the fluorescent transition was determined by transitions from the excited singlet state to a metastable state, probably the triplet state, with an intersystem crossing rate of about 0.15% and a triplet lifetime of 0 4 ms
18. F can depend on the emission dipole orientation. However, the 1 25-NA oil-immersion objective used here collected a calculated 65% of the total light emitted by a molecule at the PMMA-air interface [E H. Helen and D. Axelrod, J. Opt. Soc. Am. B 4, 337 (1987)], nearly independent of the emission-dipole orientation. The total collection or detection efficiency was 15%
19. A focused laser beam has a very small longitudinal field component along the z axis, on the order of E/kw, where E is the tangential laser field, k is 2π/λ, and w is the focused spot size [M. Lax, W. H. Louisell, W B. McKnight, Phys. Rev. A 11, 1365 (1975)].
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24. ∞ is 1 6, and the radiative lifetime is decreased for a perpendicular emission dipole
25. Aliphatic hydrocarbon immersion oil, typo FF; Cargille Laboratories, Cedar Grove, NJ
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29. -2, while continuously collecting fluorescence. It thus averaged over several hundred molecules
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