Cloning, sequencing, and expression of the gene encoding amylopullulanase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme

Applied and Environmental Microbiology

Volumn 63, Issue 9, 1997, Pages 3577-3584

  Dong, Guoqiang ^a,c   Vieille, Claire ^a   Zeikus, J Gregory ^a,b  

^a Michigan State University   (United States)

^b MICHIGAN BIOTECHNOLOGY INSTITUTE   (United States)

^c AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE SEQUENCE; GENETIC CODE; MOLECULAR CLONING; NONHUMAN; PROTEIN DENATURATION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

DICTYOGLOMUS THERMOPHILUM; ESCHERICHIA COLI; PYROCOCCUS FURIOSUS; THERMOPHILUM;

EID: 0030803307     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.9.3577-3584.1997     Document Type: Article

References (39)

1. Amylase Research Society of Japan. 1988. Handbook of amylases and related enzymes, their sources, isolation methods, properties and applications. Pergamon Press, Oxford, United Kingdom.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1993. Current protocols in molecular biology. Greene Publishing, New York, N.Y.
3. Bernfeld, P. 1955. Amylases α-and β-. Methods Enzymol. 1:149-158.
4. Brook, S. 1992. A simple computer program with statistical tests for the analysis of enzyme kinetics. Biotechniques 13:906-911.
5. Brown, J. W., C. J. Daniels, and J. N. Reeve. 1989. Gene structure, organization, and expression in Archaehacteria. Crit. Rev. Microbiol. 16:287-338.
6. Brown, S. H., H. R. Costantino, and R. M. Kelly. 1990. Characterization of amylolytic activities associated with the hyperthermophilic archaebacterium Pyrococcus furiosus. Appl. Environ. Microbiol. 56:1985-1991.
7. Brown, S. H., and R. M. Kelly. 1993. Characterization of amylolytic enzymes, having both α-1,4 and α-1,6 hydrolytic activity, from the thermophilic archaea Pyrococcus furiosus and Thermococcus litoralis. Appl. Environ. Microbiol. 59:2614-2621.
8. Burchhardt, G., A. Wienecke, and H. Bahl. 1991. Isolation of the pullulanase gene from Clostridium thermosulfurogenes (SDM 3896) and its expression in Escherichia coli. Curr. Microbiol. 22:91-95.
9. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
10. DiRuggiero, J., F. T. Robb, R. Jagus, H. H. Klump, K. M. Borgest, M. Kessel, X. Mai, and M. W. W. Adams. 1993. Characterization, cloning, and in vitro expression of the extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaeon, ES4. J. Biol. Chem. 268:17767-17774.
11. Dong, G., C. Vieille, A. Savchenko, and J. G. Zeikus. 1997. Cloning, sequencing, and expression of the gene encoding extracellular α-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme. Appl. Environ. Microbiol. 63:3569-3576.
12. Farber, G. K., and G. A. Petsko. 1990. The evolution of α/β barrel enzymes. Trends Biochem. Sci. 15:228-234.
13. Fiala, G., and K. O. Stetter. 1986. Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C. Arch. Microbiol. 145:56-61.
14. Glusker, J. P. 1991. Structural aspects of metal liganding to functional groups in proteins. Adv. Prot. Chem. 42:1-76.
15. Hain, J., W.-D. Reiter, U. Hudepohl, and W. Zillig. 1992. Elements of an archaeal promoter defined by mutational analysis. Nucleic Acids Res. 20: 5423-5428.
16. Henikoff, S. 1984. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351-359.
17. Hyun, H. H., and J. G. Zeikus. 1985. General biochemical characterization of thermostable pullulanase and glucoamylase from Clostridium thermohydrosulfuricum. Appl. Environ. Microbiol. 49:1168-1173.
18. Irving, H., and R. J. P. Williams. 1953. Stability of transition metal complexes. Chem. Soc. J. 1953:3192-3210.
19. Laderman, K. A., K. Asada, T. Uemori, H. Mukai, Y. Taguchi, I. Kato, and C. B. Anfinsen. 1993. α-Amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus, cloning and sequencing of the gene and expression in Escherichia coli. J. Biol. Chem. 268:24402-24407.
20. Mathupala, S. P., S. E. Lowe, S. M. Podkovyrov, and J. G. Zeikus. 1993. Sequencing of the amylopullulanase (apu) gene of Thermoanaerobacter ethanolicus 39E, and identification of the active site by site-directed mutagenesis. J. Biol. Chem. 268:16332-16344.
21. Mathupala, S. P., and J. G. Zeikus. 1993. Improved purification and biochemical characterization of extracellular amylopullulanase from Thermoanaerobacter ethanolicus 39E. Appl. Microbiol. Biotechnol. 39:487-493.
22. Matsuura, Y., M. Kusunoki, W. Harada, and M. Kakudo. 1984. Structure and possible catalytic residues of Taka-amylase A. J. Biochem. 95:697-702.
23. Melasniemi, H. 1988. Purification and some properties of the extracellular α-amylase-pullulanase produced by Clostridium thermohydrosulfuricum. Biochem. J. 250:813-818.
24. Perrin, D. D., and B. Dempsey (ed.). 1979. Buffers for pH and metal ion control. Chapman and Hall, Ltd., London, United Kingdom.
25. Ramesh, M. V., S. M. Podkovyrov, S. E. Lowe, and J. G. Zeikus. 1994. Cloning and sequencing of the Thermoanaerobacterium saccharolyticum B6A-RI apu gene and purification and characterization of the amylopullulanase from Escherichia coli. Appl. Environ. Microbiol. 60:94-101.
26. Rost, B., and C. Sanders. 1993. Prediction of protein structure at better than 70% accuracy. J. Mol. Biol. 232:584-599.
27. Rost, B., and C. Sanders. 1994. Combining evolutionary information and neural networks to predict protein secondary structure. Proteins 19:55-72.
28. Rost, B., C. Sanders, and R. Schneider. 1994. PHD - an automatic mail server for protein secondary structure prediction. CABIOS 10:53-60.
29. Rüdiger, A., P. L. Jorgensen, and G. Antranikian. 1995. Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli. Appl. Environ. Microbiol. 61:567-575.
30. Saha, B. C., S. P. Mathupala, and J. G. Zeikus. 1988. Purification and characterization of a highly thermostable novel pullulanase from Clostridium thermohydrosulfuricum. Biochem. J. 252:343-348.
31. Schuliger, J. W., S. H. Brown, J. A. Baross, and R. M. Kelly. 1993. Purification and characterization of a novel amylolytic enzyme from ES4, a marine hyperthermophilic archaeum. Mol. Mar. Biol. Biotechnol. 2:76-87.
32. Spreinat, A., and G. Antranikian. 1990. Purification and properties of a thermostable pullulanase from Clostridium thermosulfurogenes EM1 which hydrolyzes both α-1,6 and α-1,4-glycosidic linkages. Appl. Microbiol. Biotechnol. 33:511-518.
33. Tiboni, O., P. Cammarano, and A.M. Sanangelantoni. 1993 Cloning and sequencing of the gene encoding glutamine synthetase I from the archaeum Pyrococcus woesei: anomalous phylogenies inferred from analysis of archaeal and bacterial glutamine synthetase I sequences. J. Bacteriol. 175:2961-2969.
34. Vieille, C., D. S. Burdette, and J. G. Zeikus. 1996. Thermozymes. Biotechnol. Annu. Rev. 2:1-83.
35. Volkin, D. B., and C. R. Middaugh. 1992. The effect of temperature on protein structure, p. 215-247. In T. J. Ahern and M. C. Manning (ed.), Stability of protein pharmaceuticals, part A. Chemical and physical pathways of protein degradation. Plenum Press, New York, N.Y.
36. Voorhorst, W. G. B., R. I. L. Eggen, E. J. Luesink, and W. M. de Vos. 1995. Characterization of the celB gene coding for β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus and its expression and site-directed mutation in Escherichia coli. J. Bacteriol. 177:7105-7111.
37. Watson, M. E. E. 1984. Compilation of published signal sequences. Nucleic Acids Res. 12:5145-5264.
38. Zeikus, J. G., and B. C. Saha. 1989. Novel highly thermostable pullulanase from thermophiles. Trends Biotechnol. 7:234-239.
39. Zwickl, P., S. Fabry, C. Bogedain, A. Haas, and R. Hensel. 1990. Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli. J. Bacteriol. 172: 4329-4338.