Direct effects of endotoxin on the endothelium: Barrier function and injury

Laboratory Investigation

Volumn 79, Issue 10, 1999, Pages 1181-1199

  Bannerman, Douglas D ^a   Goldblum, Simeon E ^a,b  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b BALTIMORE VA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; BINDING PROTEIN; CD14 ANTIGEN; ENDOTOXIN; LIPID A; LIPOPOLYSACCHARIDE; PROTEIN TYROSINE KINASE;

APOPTOSIS; COLLAPSE; CYTOSKELETON; DEPOLYMERIZATION; ENDOTHELIUM; ENDOTHELIUM INJURY; GRAM NEGATIVE BACTERIUM; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS INHIBITION; REVIEW; SEPSIS;

ANIMALS; ANTIGENS, CD14; CARRIER PROTEINS; CELL MEMBRANE PERMEABILITY; ENDOTHELIUM, VASCULAR; HUMANS; LIPOPOLYSACCHARIDES; PHOSPHORYLATION; SOLUBILITY; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0032834596     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (200)

1. Abedi H and Zachary I (1997). Vascular endothelial growth factor stimulates tyrosine phosphorylation and recruitment to new focal adhesions of focal adhesion kinase and paxillin in endothelial cells. J Biol Chem 272:15442-15451.
2. Abello PA, Fidler SA, Bulkley GB, and Buchman TG (1994). Antioxidants modulate induction of programmed endothelial cell death (apoptosis) by endotoxin. Arch Surg 129:134-140.
3. Aberle H, Schwartz H, and Kemler R (1996). Cadherincatenin complex: Protein interactions and their implications for cadherin function. J Cell Biochem 61:514-523.
4. Adamson P, Tighe M, and Pearson JD (1996). Protein tyrosine kinase inhibitors act downstream of IL-1 alpha and LPS stimulated MAP-kinase phosphorylation to inhibit expression of E-selectin on human umbilical vein endothelial cells. Cell Adhes Commun 3:511-525.
5. Akarasereenont P, Mitchell JA, Appleton I, Thiemermann C, and Vane JR (1994). Involvement of tyrosine kinase in the induction of cyclo-oxygenase and nitric oxide synthase by endotoxin in cultured cells. Br J Pharmacol 113:1522-1528.
6. Albelda SM (1991). Endothelial and epithelial cell adhesion molecules. Am J Respir Cell Mol Biol 4:195-203.
7. Alexander JS, Hechtman HB, and Shepro D (1988). Phalloidin enhances endothelial barrier function and reduces inflammatory permeability in vitro. Microvasc Res 35:308-315.
8. Alpert G, Baldwin G, Thompson C, Wainwright N, Novitsky TJ, Gillis Z, Parsonnet J, Fleisher GR, and Siber GR (1992). Limulus antilipopolysaccharide factor protects rabbits from meningococcal endotoxin shock. J Infect Dis 165:494-500.
9. Arditi M and Zhou J (1997). Differential antibiotic-induced endotoxin release and interleukin-6 production by human umbilical vein endothelial cells (HUVECs): Amplification of the response by coincubation of HUVECs and blood cells. J Infect Dis 175:1255-1258.
10. Arditi M, Zhou J, Dorio R, Rong GW, Goyert SM, and Kim KS (1993). Endotoxin-mediated endothelial cell injury and activation: Role of soluble CD14. Infect Immun 61:3149-3156.
11. Arditi M, Zhou J, Torres M, Durden DL, Stins M, and Kim KS (1995). Lipopolysaccharide stimulates the tyrosine phosphorylation of mitogen-activated protein kinases p44, p42, and p41 in vascular endothelial cells in a soluble CD14-dependent manner: Role of protein tyrosine phosphorylation in lipopolysaccharide-induced stimulation of endothelial cells. J Immunol 155:3994-4003.
12. i transients and changes the organization of actin in microglia. Glia 11:336-344.
13. Bannerman DD, Fitzpatrick MJ, Anderson DY, Bhattacharjee AK, Novitsky TJ, Hasday JD, Cross AS, and Goldblum SE (1998a). Endotoxin-neutralizing protein protects against endotoxin-induced endothelial barrier dysfunction. Infect Immun 66:1400-1407.
14. Bannerman DD and Goldblum SE (1997). Endotoxin induces endothelial barrier dysfunction through protein tyrosine phosphorylation. Am J Physiol 273:L217-226.
15. Bannerman DD, Sathyamoorthy M, and Goldblum SE (1998b). Bacterial lipopolysaccharide disrupts endothelial monolayer integrity and survival signaling events through caspase cleavage of adherens junction proteins. J Biol Chem 273:35371-35380.
16. Barth AI, Nathke IS, and Nelson WJ (1997). Cadherins, catenins and APC protein: Interplay between cytoskeletal complexes and signaling pathways. Curr Opin Cell Biol 9:683-690.
17. Battafaraono RJ, Dahlberg PS, Ratz CA, Johnston JW, Gray BH, Haseman JR, Mayo KH, and Dunn DL (1995). Peptide derivatives of three distinct lipopolysaccharide binding proteins inhibit lipopolysaccharide-induced tumor necrosis factor-alpha secretion in vitro. Surgery 118:318-324.
18. Beaty CD, Franklin TL, Uehara Y, and Wilson CB (1994). Lipopolysaccharide-induced cytokine production in human monocytes: Role of tyrosine phosphorylation in transmembrane signal transduction. Eur J Immunol 24:1278-1284.
19. Brandtzaeg P, Kierulf P, Gaustad P, Skulberg A, Bruun JN, Halvorsen S, and Sorensen E (1989). Plasma endotoxin as a predictor of multiple organ failure and death in systemic meningococcal disease. J Infect Dis 159:195-204.
20. Brigham KL and Meyrick B (1986). Endotoxin and lung injury. Am Rev Respir Dis 133:913-927.
21. Buchman TG, Abello PA, Smith EH, and Bulkley GB (1993). Induction of heat shock response leads to apoptosis in endothelial cells previously exposed to endotoxin. Am J Physiol 265:H165-H170.
22. Bufler P, Stiegler G, Schuchmann M, Hess S, Kruger C, Stelter F, Eckerskorn C, Schutt C, and Engelmann H (1995). Soluble lipopolysaccharide receptor (CD14) is released via two different mechanisms from human monocytes and CD14 transfectants. Eur J Immunol 25:604-610.
23. Burridge K, Fath K, Kelly T, Nuckolls G, and Turner C (1988). Focal adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu Rev Cell Biol 4:487-525.
24. Burridge K, Turner CE, and Romer LH (1992). Tyrosine phosphorylation of paxillin and pp 125FAK accompanies cell adhesion to extracellular matrix: A role in cytoskeletal assembly. J Cell Biol 119:893-903.
25. Bursten SL, Stevenson F, Torrano F, and Lovett DH (1991). Mesangial cell activation by bacterial endotoxin: Induction of rapid cytoskeletal reorganization and gene expression. Am J Pathol 139:371-382.
26. Bussolino F, Camussi G, Aglietta M, Braquet P, Bosia A, Pescarmona G, Sanavio F, D'Urso N, and Marchisio PC (1987). Human endothelial cells are targets for platelet-activating factor. I. Platelet-activating factor induces changes in cytoskeleton structures. J Immunol 139:2439-2446.
27. Carlos TM and Harlan JM (1994). Leukocyte-endothelial adhesion molecules. Blood 84:2068-2101.
28. Chang SW, Feddersen CO, Henson PM, and Voelkel NF (1987). Platelet-activating factor mediates hemodynamic changes and lung injury in endotoxin-treated rats. J Clin Invest 79:1498-1509.
29. Choi KB, Wong F, Harlan JM, Chaudhary PM, Hood L, and Karsan A (1998). Lipopolysaccharide mediates endothelial apoptosis by a FADD-dependent pathway. J Biol Chem 273:20185-20188.
30. Chow CW, Grinstein S, and Rotstein OD (1995). Signaling events in monocytes and macrophages. New Horiz 3:342-351.
31. Colucci M, Balconi G, Lorenzet R, Pietra A, Locati D, Donati MB, and Semeraro N (1983). Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 71:1893-1896.
32. Cooperstock MS (1974). Inactivation of endotoxin by polymyxin B. Antimicrob Agents Chemother 6:422-425.
33. Crowley MT, Harmer SL, and DeFranco AL (1996). Activation-induced association of a 145-kDa tyrosine-phosphorylated protein with She and Syk in B lymphocytes and macrophages. J Biol Chem 271:1145-1152.
34. Dejana E (1997). Endothelial adherens junctions: Implications in the control of vascular permeability and angiogenesis. J Clin Invest 100:S7-S10.
35. Dejana E, Corada M, and Lampugnani MG (1995). Endothelial cell-to-cell junctions. FASEB J 9:910-918.
36. Dejana E and Del Maschio A (1995). Molecular organization and functional regulation of cell to cell junctions in the endothelium. Thromb Haemost 74:309-312.
37. Dentener MA, Bazil V, Von Asmuth EJ, Ceska M, and Buurman WA (1993). Involvement of CD14 in lipopolysaccharide-induced tumor necrosis factor-alpha, IL-6 and IL-8 release by human monocytes and alveolar macrophages. J Immunol 150:2885-2891.
38. Desch CE, O'Hara P, and Harlan JM (1989). Antilipopolysaccharide factor from horseshoe crab, Tachypleus tridentatus, inhibits lipopolysaccharide activation of cultured human endothelial cells. Infect Immun 57:1612-1614.
39. Dong Z, O'Brian CA, and Fidler IJ (1993a). Activation of tumoricidal properties in macrophages by lipopolysaccharide requires protein-tyrosine kinase activity. J Leukoc Biol 53:53-60.
40. Dong Z, Qi X, and Fidler IJ (1993b). Tyrosine phosphorylation of mitogen-activated protein kinases is necessary for activation of murine macrophages by natural and synthetic bacterial products. J Exp Med 177:1071-1077.
41. Dong Z, Qi X, Xie K, and Fidler IJ (1993c). Protein tyrosine kinase inhibitors decrease induction of nitric oxide synthase activity in lipopolysaccharide-responsive and lipopolysaccharide-nonresponsive murine macrophages. J Immunol 151:2717-2724.
42. Duchow J, Marchant A, Crusiaux A, Husson C, Alonso-Vega C, De Groote D, Neve P, and Goldman M (1993). Impaired phagocyte responses to lipopolysaccharide in paroxysmal nocturnal hemoglobinuria. Infect Immun 61:4280-4285.
43. Durieux JJ, Vita N, Popescu O, Guette F, Calzada-Wack J, Munker R, Schmidt RE, Lupker J, Ferrara P, Ziegler-Heitbrock HW, and Labeta MO (1994). The two soluble forms of the lipopolysaccharide receptor, CD14: Characterization and release by normal human monocytes. Eur J Immunol 24:2006-2012.
44. Dziarski R, Jin YP, and Gupta D (1996). Differential activation of extracellular signal-regulated kinase (ERK) 1, ERK2, p38, and c-Jun NH2-terminal kinase mitogen-activated protein kinases by bacterial peptidoglycan. J Infect Dis 174:777-785.
45. English BK, Ihle JN, Myracle A, and Yi T (1993). Hck tyrosine kinase activity modulates tumor necrosis factor production by murine macrophages. J Exp Med 178:1017-1022.
46. Felice GR, Eason P, Nermut MV, and Kellie S (1990). pp 60v-src association with the cytoskeleton induces actin reorganization without affecting polymerization status. Eur J Cell Biol 52:47-59.
47. Fletcher MA, McKenna TM, Quance JL, Wainwright NR, and Williams TJ (1993). Lipopolysaccharide detoxification by endotoxin neutralizing protein. J Surg Res 55:147-154.
48. Flick MR, Horn JK, Hoeffel JM, and Goldstein IM (1986). Reduction of total hemolytic complement activity with Naja haje cobra venom factor does not prevent endotoxin-induced lung injury in sheep. Am Rev Respir Dis 133:62-67.
49. Frey EA and Finlay BB (1998). Lipopolysaccharide induces apoptosis in a bovine endothelial cell line via a soluble CD14 dependent pathway. Microb Pathog 24:101-109.
50. Frey EA, Miller DS, Jahr TG, Sundan A, Bazil V, Espevik T, Finlay BB, and Wright SD (1992). Soluble CD14 participates in the response of cells to lipopolysaccharide. J Exp Med 176:1665-1671.
51. Fujita M, Kuwano K, Kunitake R, Hagimoto N, Miyazaki H, Kaneko Y, Kawasaki M, Maeyama T, and Hara N (1998). Endothelial cell apoptosis in lipopolysaccharide-induced lung injury in mice. Int Arch Allergy Immunol 117:202-208.
52. Gallay P, Neumann D, Le Roy D, Barras C, and Glauser MP (1993). Lipopolysaccharide-binding protein as a major plasma protein responsible for endotoxemic shock. Proc Natl Acad Sci USA 90:9935-9938.
53. Garcia JG and Schaphorst KL (1995). Regulation of endothelial cell gap formation and paracellular permeability. J Investig Med 43:117-126.
54. Gaynor E, Bouvier C, and Spaet TH (1970). Vascular lesions: Possible pathogenetic basis of the generalized Shwartzman reaction. Science 170:986-988.
55. Geng Y, Gulbins E, Altman A, and Lotz M (1994). Monocyte deactivation by interleukin 10 via inhibition of tyrosine kinase activity and the Ras signaling pathway. Proc Natl Acad Sci USA 91:8602-8606.
56. Geng Y, Zhang B, and Lotz M (1993). Protein tyrosine kinase activation is required for lipopolysaccharide induction of cytokines in human blood monocytes. J Immunol 151:6692-6700.
57. Geppert TD, Whitehurst CE, Thompson P, and Beutler B (1994). Lipopolysaccharide signals activation of tumor necrosis factor biosynthesis through the ras/raf-1/MEK/MAPK pathway. Mol Med 1:93-103.
58. Glaser KB, Sung A, Bauer J, and Weichman BM (1993). Regulation of eicosanoid biosynthesis in the macrophage: Involvement of protein tyrosine phosphorylation and modulation by selective protein tyrosine kinase inhibitors. Biochem Pharmacol 45:711-721.
59. Goldblum SE, Brann TW, Ding X, Pugin J, and Tobias PS (1994). Lipopolysaccharide (LPS)-binding protein and soluble CD14 function as accessory molecules for LPS-induced changes in endothelial barrier function, in vitro. J Clin Invest 93:692-702.
60. Goldblum SE, Ding X, Brann TW, and Campbell-Washington J (1993a). Bacterial lipopolysaccharide induces actin reorganization, intercellular gap formation, and endothelial barrier dysfunction in pulmonary vascular endothelial cells: Concurrent F-actin depolymerization and new actin synthesis. J Cell Physiol 157:13-23.
61. Goldblum SE, Ding X, and Campbell-Washington J (1993b). TNF-alpha induces endothelial cell F-actin depolymerization, new actin synthesis, and barrier dysfunction. Am J Physiol 264:C894-905.
62. Golenbock DT, Liu Y, Millham FH, Freeman MW, and Zoeller RA (1993). Surface expression of human CD14 in Chinese hamster ovary fibroblasts imparts macrophage-like responsiveness to bacterial endotoxin. J Biol Chem 268:22055-22059.
63. Gotlieb AI (1990). The endothelial cytoskeleton: Organization in normal and regenerating endothelium. Toxicol Pathol 18: 603-617.
64. Gottlieb AI, Langille BL, Wong MK, and Kim DW (1991). Structure and function of the endothelial cytoskeleton. Lab Invest 65:123-137.
65. Graham IL, Anderson DC, Holers VM, and Brown EJ (1994). Complement receptor 3 (CR3, Mac-1, integrin alpha M beta 2, CD11b/CD18) is required for tyrosine phosphorylation of paxillin in adherent and nonadherent neutrophils. J Cell Biol 127:1139-1147.
66. Haimovitz-Friedman A, Cordon-Cardo C, Bayoumy S, Garzotto M, McLoughlin M, Gallily R, Edwards CK 3rd, Schuchman EH, Fuks Z, and Kolesnick R (1997). Lipopolysaccharide induces disseminated endothelial apoptosis requiring ceramide generation. J Exp Med 186:1831-1841.
67. Han J, Lee JD, Bibbs L, and Ulevitch RJ (1994). A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 265:808-811.
68. Han J, Lee JD, Tobias PS, and Ulevitch RJ (1993). Endotoxin induces rapid protein tyrosine phosphorylation in 70Z/3 cells expressing CD14. J Biol Chem 268:25009-25014.
69. Harlan JM, Harker LA, Reidy MA, Gajdusek CM, Schwartz SM, and Striker GE (1983a). Lipopolysaccharide-mediated bovine endothelial cell injury in vitro. Lab Invest 48:269-274.
70. Harlan JM, Harker LA, Striker GE, and Weaver LJ (1983b). Effects of lipopolysaccharide on human endothelial cells in culture. Thromb Res 29:15-26.
71. Haziot A, Ferrero E, Kontgen F, Hijiya N. Yamamoto S, Silver J, Stewart CL, and Goyert SM (1996). Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity 4:407-414.
72. Herrera-Velit P, Knutson KL, and Reiner NE (1997). Phosphatidylinositol 3-kinase-dependent activation of protein kinase C-zeta in bacterial lipopolysaccharide-treated human monocytes. J Biol Chem 272:16445-16452.
73. Herrera-Velit P and Reiner NE (1996). Bacterial lipopolysaccharide induces the association and coordinate activation of p53/56lyn and phosphatidylinositol 3-kinase in human monocytes. J Immunol 156:1157-1165.
74. Hinshaw LB (1996). Sepsis/septic shock: Participation of the microcirculation - An abbreviated review. Crit Care Med 24: 1072-1078.
75. Hitchcock PJ, Leive L, Makela PH, Rietschel BT, Strittmatter W, and Morrison DC (1986). Lipopolysaccharide nomenclature: Past, present, and future. J Bacteriol 166: 699-705.
76. Hoffmann JA, Kafatos FC, Janeway CA, and Ezekowitz RAB (1999). Phylogenetic perspectives in innate immunity. Science 284:1313-1318.
77. Holme TC, Kellie S, Wyke JA, and Crawford N (1986). Effect of transformation by Rous sarcoma virus on the character and distribution of actin in Rat-1 fibroblasts: A biochemical and microscopical study. Br J Cancer 53:465-476.
78. Hotchkiss RS, Swanson PE, Cobb JP, Jacobson A, Buchman TG, and Karl IE (1997). Apoptosis in lymphoid and parenchymal cells during sepsis: Findings in normal and T-and B-cell-deficient mice. Crit Care Med 25:1298-1307.
79. Howard PK, Sefton BM, and Firtel RA (1993). Tyrosine phosphorylation of actin in Dictyostelium associated with cell-shape changes. Science 259:241-244.
80. Hoyt DG, Mannix RJ, Gerritsen ME, Watkins SC, Lazo JS, and Pitt BR (1996). Integrins inhibit LPS-induced DNA strand breakage in cultured lung endothelial cells. Am J Physiol 270:L689-L694.
81. Hoyt DG, Mannix RJ, Rusnak JM, Pitt BR, and Lazo JS (1995). Collagen is a survival factor against LPS-induced apoptosis in cultured sheep pulmonary artery endothelial cells. Am J Physiol 269:L171-L177.
82. Hu X, Yee E, Harlan JM, Wong F, and Karsan A (1998). Lipopolysaccharide induces the antiapoptotic molecules, A1 and A20, in microvascular endothelial cells. Blood 92:2759-2765.
83. Ishii Y, Shuyi W, and Kitamura S (1995). Soluble CD14 in serum mediates LPS-induced increase in permeability of bovine pulmonary arterial endothelial cell monolayers in vitro. Life Sci 56:2263-2272.
84. Jack RS, Fan X, Bernheiden M, Rune G, Ehlers M, Weber A, Kirsch G, Mentel R, Furll B, Freudenberg M, Schmitz G, Stelter F, and Schutt C (1997). Lipopolysaccharide-binding protein is required to combat a murine gram-negative bacterial infection. Nature 389:742-745.
85. Jirik FR, Podor TJ, Hirano T, Kishimoto T, Loskutoff DJ, Carson DA, and Lotz M (1989). Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J Immunol 142:144-147.
86. Karsan A and Harlan JM (1996). Modulation of endothelial cell apoptosis: Mechanisms and pathophysiological roles. J Atheroscler Thromb 3:75-80.
87. Karsan A, Yee E, and Harlan JM (1996). Endothelial cell death induced by tumor necrosis factor-alpha is inhibited by the Bcl-2 family member, A1. J Biol Chem 271:27201-27204.
88. Kirschning CJ, Wesche H, Merrill Ayres T, and Rothe M (1998). Human toll-like receptor 2 confers responsiveness to bacterial lipopolysaccharide. J Exp Med 188:2091-2097.
89. Kopp EB and Medzhitov R (1999). The Toll-receptor family and control of innate immunity. Curr Opin Immunol 11:13-18.
90. Koshi R, Mathan VI, David S, and Mathan MM (1993). Enteric vascular endothelial response to bacterial endotoxin. Int J Exp Pathol 74:593-601.
91. Kroemer G, Petit P, Zamzami N, Vayssiere JL, and Mignotte B (1995). The biochemistry of programmed cell death. FASEB J 9:1277-1287.
92. Kuo PC, Schroeder RA, and Bartlett ST (1997). Endotoxin-mediated synthesis of nitric oxide is dependent on Gq protein signal transduction. Surgery 122:394-402.
93. Labeta MO, Durieux JJ, Fernandez N, Herrmann R, and Ferrara P (1993). Release from a human monocyte-like cell line of two different soluble forms of the lipopolysaccharide receptor, CD14. Eur J Immunol 23:2144-2151.
94. Leeb-Lundberg LM, Song XH, and Mathis SA (1994). Focal adhesion-associated proteins p125FAK and paxillin are substrates for bradykinin-stimulated tyrosine phosphorylation in Swiss 3T3 cells. J Biol Chem 269:24328-24334.
95. Leturcq DJ, Moriarty AM, Talbott G, Winn RK, Martin TR, and Ulevitch RJ (1996). Antibodies against CD14 protect primates from endotoxin-induced shock. J Clin Invest 98:1533-1538.
96. Levi M, ten Cate H, van der Poll T, and van Deventer SJ (1993). Pathogenesis of disseminated intravascular coagulation in sepsis. JAMA 270:975-979.
97. Li P, Allen H, Banerjee S, Franklin S, Herzog L, Johnston C, McDowell J, Paskind M, Rodman L, Salfeld J, Towne E, Tracey D, Wardwell S, Wei F, Wong W, Kamen R, and Seshadri T (1995). Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell 80:401-411.
98. Li P, Allen H, Banerjee S, and Seshadri T (1997). Characterization of mice deficient in interleukin-1 beta converting enzyme. J Cell Biochem 64:27-32.
99. Liu MK, Herrera-Velit P, Brownsey RW, and Reiner NE (1994). CD14-dependent activation of protein kinase C and mitogen-activated protein kinases (p42 and p44) in human monocytes treated with bacterial lipopolysaccharide. J Immunol 153:2642-2652.
100. Lo CJ, Cryer HG, and Maier RV (1996). Prostaglandin E2 production by endotoxin-stimulated alveolar macrophages is regulated by phospholipase C pathways. J Trauma 40:557-562.
101. Lum H and Malik AB (1994). Regulation of vascular endothelial barrier function. Am J Physiol 267:L223-L241.
102. Lum H and Malik AB (1996). Mechanisms of increased endothelial permeability. Can J Physiol Pharmacol 74:787-800.
103. Lynn WA, Raetz CR, Qureshi N, and Golenbock DT (1991). Lipopolysaccharide-induced stimulation of CD11b/CD18 expression on neutrophils: Evidence of specific receptor-based response and inhibition by lipid A-based antagonists. J Immunol 147:3072-3079.
104. Maher PA, Pasquale EB, Wang JY, and Singer SJ (1985). Phosphotyrosine-containing proteins are concentrated in focal adhesions and intercellular junctions in normal cells. Proc Natl Acad Sci USA 82:6576-6580.
105. Martich GD, Boujoukos AJ, and Suffredini AF (1993). Response of man to endotoxin. Immunobiology 187:403-416.
106. Martin MA and Silverman HJ (1992). Gram-negative sepsis and the adult respiratory distress syndrome. Clin Infect Dis 14:1213-1228.
107. Mattsson E, Van Dijk H, Van Kessel K, Verhoef J, Fleer A, and Rollof J (1996). Intracellular pathways involved in tumor necrosis factor-alpha release by human monocytes on stimulation with lipopolysaccharide or staphylococcal peptidoglycan are partly similar. J Infect Dis 173:212-218.
108. Melzig MF and Loose R (1995). Investigations into the mechanism of toxicity of lipopolysaccharide (LPS) in bovine aortic endothelial cells. Pharmazie 50:558-560.
109. Meng F and Lowell CA (1997). Lipopolysaccharide (LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinases Hck, Fgr, and Lyn. J Exp Med 185:1661-1670.
110. Meyrick B (1986a). Pathology of the adult respiratory distress syndrome. Crit Care Clin 2:405-428.
111. Meyrick B, Berry LC Jr, and Christman BW (1995). Response of cultured human pulmonary artery endothelial cells to endotoxin. Am J Physiol 268:L239-L244.
112. Meyrick B and Brigham KL (1983). Acute effects of Escherichia coli endotoxin on the pulmonary microcirculation of anesthetized sheep structure:function relationships. Lab Invest 48:458-470.
113. Meyrick B, Hoover R, Jones MR, Berry LC Jr, and Brigham KL (1989). In vitro effects of endotoxin on bovine and sheep lung microvascular and pulmonary artery endothelial cells. J Cell Physiol 138:165-174.
114. Meyrick BO (1986b). Endotoxin-mediated pulmonary endothelial cell injury. Fed Proc 45:19-24.
115. Meyrick BO, Ryan US, and Brigham KL (1986). Direct effects of E coli endotoxin on structure and permeability of pulmonary endothelial monolayers and the endothelial layer of intimal explants. Am J Pathol 122:140-151.
116. Molitoris BA (1997). Putting the actin cytoskeleton into perspective: Pathophysiology of ischemic alterations. Am J Physiol 272:F430-F433.
117. Morita T, Ohtsubo S, Nakamura T, Tanaka S, Iwanaga S, Ohashi K, and Niwa M (1985). Isolation and biological activities of limulus anticoagulant (anti-LPS factor) which interacts with lipopolysaccharide (LPS). J Biochem (Tokyo) 97:1611-1620.
118. Morrison DC, Dinarello CA, Munford RS, Natanson C, Danner R, Pollack M, Spitzer JJ, Ulevitch RJ, Vogel SN, and McSweegan E (1994). Current status of bacterial endotoxins. ASM News 60:479-484.
119. Morrison DC and Jacobs DM (1976). Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry 13:813-818.
120. Muroi M and Suzuki T (1993). Role of protein kinase A in LPS-induced activation of NF-kappa B proteins of a mouse macrophage-like cell line, J774. Cell Signal 5:289-298.
121. Musso T, Johnston JA, Linnekin D, Varesio L, Rowe TK, JJ OS, and McVicar DW (1995). Regulation of JAK3 expression in human monocytes: Phosphorylation in response to interleukins 2:4, and 7. J Exp Med 181:1425-1431.
122. Nishiya T, Uehara T, and Nomura Y (1995). Herbimycin A suppresses NF-kappa B activation and tyrosine phosphorylation of JAK2 and the subsequent induction of nitric oxide synthase in C6 glioma cells. FEBS Lett 371:333-336.
123. Novogrodsky A, Vanichkin A, Patya M, Gazit A, Osherov N, and Levitzki A (1994). Prevention of lipopolysaccharide-induced lethal toxicity by tyrosine kinase inhibitors. Science 264:1319-1322.
124. Olson NC, Hellyer PW, and Dodam JR (1995). Mediators and vascular effects in response to endotoxin. Br Vet J 151:489-522.
125. Pajkrt D and van Deventer SJ (1996). The cellular response in sepsis. CurrTop Microbiol Immunol 216:119-132.
126. Parsons PE, Worthen GS, Moore EE, Tate RM, and Henson PM (1989). The association of circulating endotoxin with the development of the adult respiratory distress syndrome. Am Rev Respir Dis 140:294-301.
127. Patrick D, Betts J, Frey EA, Prameya R, Dorovini-Zis K, and Finlay BB (1992). Haemophilus influenzae lipopolysaccharide disrupts confluent monolayers of bovine brain endothelial cells via a serum-dependent cytotoxic pathway. J Infect Dis 165:865-872.
128. Phillips PG, Lum H, Malik AB, and Tsan MF (1989). Phallacidin prevents thrombin-induced increases in endothelial permeability to albumin. Am J Physiol 257:C562-C567.
129. Pingleton WW, Coalson JJ, and Guenter CA (1975). Significance of leukocytes in endotoxin shock. Exp Mol Pathol 22:183-194.
130. Podolski JL, Mooteri SN, Drab-Weiss EA, Onoda JM, Saclarides TJ, and Rubin DB (1998). Aminothiol WR-1065 protects endothelial cell morphology against alterations induced by lipopolysaccharide. Shock 10:430-435.
131. Pohlman TH, Stanness KA, Beatty PG, Ochs HD, and Harlan JM (1986). An endothelial cell surface factor(s) induced in vitro by lipopolysaccharide, interleukin 1, and tumor necrosis factor-alpha increases neutrophil adherence by a CDw18-dependent mechanism. J Immunol 136:4548-4553.
132. Pollack M and Ohl CA (1996). Endotoxin-based molecular strategies for the prevention and treatment of gram-negative sepsis and septic shock. Curr Top Microbiol Immunol 216: 275-297.
133. Poltorak A, He X, Smirnova I, Liu MY, Huffel CV, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, and Beutler B (1998). Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: Mutations in Tlr4 gene. Science 282:2085-2088.
134. Prpic V, Weiel JE, Somers SD, DiGuiseppi J, Gonias SL, Pizzo SV, Hamilton TA, Herman B, and Adams DO (1987). Effects of bacterial lipopolysaccharide on the hydrolysis of phosphatidylinositol-4,5-bisphosphate in murine peritoneal macrophages. J Immunol 139:526-533.
135. Pugin J, Schurer-Maly CC, Leturcq D, Moriarty A, Ulevitch RJ, and Tobias PS (1993). Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD14. Proc Natl Acad Sci USA 90:2744-2748.
136. Raetz CR, Ulevitch RJ, Wright SD, Sibley CH, Ding A, and Nathan CF (1991). Gram-negative endotoxin: An extraordinary lipid with profound effects on eukaryotic signal transduction. FASEB J 5:2652-2660.
137. Reidy MA and Schwartz SM (1983). Endothelial injury and regeneration. IV. Endotoxin: A nondenuding injury to aortic endothelium. Lab Invest 48:25-34.
138. Reimann T, Buscher D, Hipskind RA, Krautwald S, Lohmann-Matthes ML, and Baccarini M (1994). Lipopolysaccharide induces activation of the Raf-1/MAP kinase pathway: A putative role for Raf-1 in the induction of the IL-1 beta and the TNF-alpha genes. J Immunol 153:5740-5749.
139. Retta SF, Barry ST, Critchley DR, Defilippi P, Silengo L, and Tarone G (1996). Focal adhesion and stress fiber formation is regulated by tyrosine phosphatase activity. Exp Cell Res 229:307-317.
140. Rietschel ET and Brade H (1992). Bacterial endotoxins. Sci Am 267:54-61.
141. Rietschel ET, Brade H, Holst O, Brade L, Muller-Loennies S, Mamat U, Zahringer U, Beckmann F, Seydel U, Brandenburg K, Ulmer AJ, Mattern T, Heine H, Schletter J, Loppnow H, Schonbeck U, Flad HD, Hauschildt S, Schade UF, Di Padova F, Kusumoto S, and Schumann RR (1996). Bacterial endotoxin: Chemical constitution, biological recognition, host response, and immunological detoxification. Curr Top Microbiol Immunol 216:39-81.
142. Rietschel ET, Kirikae T, Schade FU, Mamat U, Schmidt G, Loppnow H, Ulmer AJ, Zahringer U, Seydel U, Di Padova F, Schreier M, and Brade H (1994). Bacterial endotoxin: Molecular relationships of structure to activity and function. FASEB J 8:217-225.
143. Rietschel ET, Kirikae T, Schade FU, Ulmer AJ, Holst O, Brade H, Schmidt G, Mamat U, Grimmecke HD, Kusumoto S, and Hringer U (1993). The chemical structure of bacterial endotoxin in relation to bioactivity. Immunobiology 187:169-190.
144. Rietschel ET, Seydel U, Zahringer U, Schade UF, Brade L, Loppnow H, Feist W, Wang MH, Ulmer AJ, Flad HD, Brandenburg K, Kirikae T, Grimmecke D, Holst O, and Brade H (1991). Bacterial endotoxin: Molecular relationships between structure and activity. Infect Dis Clin North Am 5:753-779.
145. Rotrosen D and Gallin JI (1986). Histamine type I receptor occupancy increases endothelial cytosolic calcium, reduces F-actin, and promotes albumin diffusion across cultured endothelial monolayers. J Cell Biol 103:2379-2387.
146. Rubenstein HS, Fine J, and Coons AH (1962). Localization of endotoxin in the walls of the peripheral vascular system during lethal endotoxemia. Proc Soc Exp Biol Med 111:458-467.
147. Sakamoto M, Ino Y, Ochiai A, Kanai Y, Akimoto S, and Hirohashi S (1996). Formation of focal adhesion and spreading of polarized human colon cancer cells in association with tyrosine phosphorylation of paxillin in response to phorbol ester. Lab Invest 74:199-208.
148. Sawdey M, Podor TJ, and Loskutoff DJ (1989). Regulation of type 1 plasminogen activator inhibitor gene expression in cultured bovine aortic endothelial cells: Induction by transforming growth factor-beta, lipopolysaccharide, and tumor necrosis factor-alpha. J Biol Chem 264:10396-10401.
149. Schimke J, Mathison J, Morgiewicz J, and Ulevitch RJ (1998). Anti-CD14 mAb treatment provides therapeutic benefit after in vivo exposure to endotoxin. Proc Natl Acad Sci USA 95:13875-13880.
150. Schletter J, Heine H, Ulmer AJ, and Rietschel ET (1995). Molecular mechanisms of endotoxin activity. Arch Microbiol 164:383-389.
151. Schumann RR, Belka C, Reuter D, Lamping N, Kirschning CJ, Weber JR, and Pfeil D (1998). Lipopolysaccharide activates caspase-1 (interleukin-1-converting enzyme) in cultured monocytic and endothelial cells. Blood 91:577-584.
152. Schumann RR, Lamping N, Kirschning C, Knopf HP, Hoess A, and Herrmann F (1994). Lipopolysaccharide binding protein: Its role and therapeutical potential in inflammation and sepsis. Biochem Soc Trans 22:80-82.
153. Schumann RR, Leong SR, Flaggs GW, Gray PW, Wright SD, Mathison JC, Tobias PS, and Ulevitch RJ (1990). Structure and function of lipopolysaccharide binding protein. Science 249:1429-1431.
154. Schumann RR, Pfeil D, Lamping N, Kirschning C, Scherzinger G, Schlag P, Karawajew L, and Herrmann F (1996). Lipopolysaccharide induces the rapid tyrosine phosphorylation of the mitogen-activated protein kinases erk-1 and p38 in cultured human vascular endothelial cells requiring the presence of soluble CD14. Blood 87:2805-2814.
155. Schweiger A, Mihalache O, Ecke M, and Gerisch G (1992). Stage-specific tyrosine phosphorylation of actin in Dictyostelium discoideum cells. J Cell Sci 102:601-609.
156. Sevransky JE, Shaked G, Novogrodsky A, Levitzki A, Gazit A, Hoffman A, Elin RJ, Quezado ZM, Freeman BD, Eichacker PQ, Danner RL, Banks SM, Bacher J, Thomas ML 3rd, and Natanson C (1997). Tyrphostin AG 556 improves survival and reduces multiorgan failure in canine Escherichia coli peritonitis. J Clin Invest 99:1966-1973.
157. Shapira L, Takashiba S, Champagne C, Amar S, and Van Dyke TE (1994). Involvement of protein kinase C and protein tyrosine kinase in lipopolysaccharide-induced TNF-alpha and IL-1 beta production by human monocytes. J Immunol 153:1818-1824.
158. Shasby DM, Shasby SS, Sullivan JM, and Peach MJ (1982). Role of endothelial cell cytoskeleton in control of endothelial permeability. Circ Res 51:657-661.
159. Shinji H, Kaiho S, Nakano T, and Yoshida T (1991). Reorganization of microfilaments in macrophages after LPS stimulation. Exp Cell Res 193:127-133.
160. Shinomiya H, Hagi A, Fukuzumi M, Mizobuchi M, Hirata H, and Utsumi S (1995). Complete primary structure and phosphorylation site of the 65-kDa macrophage protein phosphorylated by stimulation with bacterial lipopolysaccharide. J Immunol 154:3471-3478.
161. Snapper JR, Hinson JM, Hutchison AA, Lefferts PL, Ogletree ML, and Brigham KL (1984). Effects of platelet depletion on the unanesthetized sheep's pulmonary response to endotoxemia. J Clin Invest 74:1782-1791.
162. Soejima K, Ishizaka A, Urano T, Sayama K, Sakamaki F, Nakamura H, Terashima T, Waki Y, Tasaka S, Fujishima S, Kawata T, Christ WJ, and Kanazawa M (1996). Protective effect of B464, a lipid A analog, on endotoxin-induced cellular responses and acute lung injury. Am J Respir Crit Care Med 154:900-906.
163. Soldi R, Sanavio F, Aglietta M, Primo L, Defilippi P, Marchisio PC, and Bussolino F (1996). Platelet-activating factor (PAF) induces the early tyrosine phosphorylation of focal adhesion kinase (p125FAK) in human endothelial cells. Oncogene 13:515-525.
164. Stefanova I, Corcoran ML, Horak EM, Wahl LM, Bolen JB, and Horak ID (1993). Lipopolysaccharide induces activation of CD14-associated protein tyrosine kinase p53/56lyn. J Biol Chem 268:20725-20728.
165. Stelter F, Pfister M, Bernheiden M, Jack RS, Bufler P, Engelmann H, and Schutt C (1996). The myeloid differentiation antigen CD14 is N-and
166. Stern DM, Bank I, Nawroth PP, Cassimeris J, Kisiel W, Fenton JW 2d, Dinarello C, Chess L, and Jaffe EA (1985). Self-regulation of procoagulant events on the endothelial cell surface. J Exp Med 162:1223-1235.
167. Suffredini AF, Shelhamer JH, Neumann RD, Brenner M, Baltaro RJ, and Parrillo JE (1992). Pulmonary and oxygen transport effects of intravenously administered endotoxin in normal humans. Am Rev Respir Dis 145:1398-1403.
168. Suttorp N, Polley M, Seybold J, Schnittler H, Seeger W, Grimminger F, and Aktories K (1991). Adenosine diphosphate-ribosylation of G-actin by botulinum C2 toxin increases endothelial permeability in vitro. J Clin Invest 87:1575-1584.
169. Tamura DY, Moore EE, Johnson JL, Zallen G, Aiboshi J, and Silliman CC (1998a). p38 mitogen-activated protein kinase inhibition attenuates intercellular adhesion molecule-1 up-regulation on human pulmonary microvascular endothelial cells. Surgery 124:403-407.
170. Tamura M, Gu J, Matsumoto K, Aota S, Parsons R, and Yamada KM (1998b). Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN. Science 280:1614-1617.
171. Tanke T, van de Loo JW, Rhim H, Leventhal PS, Proctor RA, and Bertics PJ (1991). Bacterial lipopolysaccharide-stimulated GTPase activity in RAW 264.7 macrophage membranes. Biochem J 277:379-385.
172. Tapping RI and Tobias PS (1997). Cellular binding of soluble CD14 requires lipopolysaccharide (LPS) and LPS-binding protein. J Biol Chem 272:23157-23164.
173. Thijs LG, de Boer JP, de Groot MC, and Hack CE (1993). Coagulation disorders in septic shock. Intensive Care Med 19:S8-S15.
174. Thornberry NA and Lazebnik Y (1998). Caspases: Enemies within. Science 281:1312-1316.
175. Turner CE (1994). Paxillin: A cytoskeletal target for tyrosine kinases. Bioessays 16:47-52.
176. Turner CE, Pietras KM, Taylor DS, and Molloy CJ (1995). Angiotensin II stimulation of rapid paxillin tyrosine phosphorylation correlates with the formation of focal adhesions in rat aortic smooth muscle cells. J Cell Sci 108:333-342.
177. Uehara Y, Hori M, Takeuchi T, and Umezawa H (1986). Phenotypic change from transformed to normal induced by benzoquinonoid ansamycins accompanies inactivation of p60src in rat kidney cells infected with Rous sarcoma virus. Mol Cell Biol 6:2198-2206.
178. Ulevitch RJ (1999). Endotoxin opens the Tollgates to innate immunity. Nat Med 5:144-145.
179. Ulevitch RJ, Cochrane CG, Henson PM, Morrison DC, and Doe WF (1975). Mediation systems in bacterial lipopolysaccharide-induced hypotension and disseminated intravascular coagulation. I. The role of complement. J Exp Med 142:1570-1590.
180. Ulevitch RJ and Tobias PS (1995). Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annu Rev Immunol 13:437-457.
181. Ulevitch RJ and Tobias PS (1994). Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol 6:125-130.
182. Vanichkin A, Patya M, Gazit A, Levitzki A, and Novogrodsky A (1996). Late administration of a lipophilic tyrosine kinase inhibitor prevents lipopolysaccharide and Escherichia coli-induced lethal toxicity. J Infect Dis 173:927-933.
183. Viriyakosol S and Kirkland T (1995). Knowledge of cellular receptors for bacterial endotoxin - 1995. Clin Infect Dis 21(Suppl 2):S190-S195.
184. Volberg T, Zick Y, Dror R, Sabanay I, Gilon C, Levitzki A, and Geiger B (1992). The effect of tyrosine-specific protein phosphorylation on the assembly of adherens-type junctions. EMBO J 11:1733-1742.
185. Wainwright NR, Miller RJ, Paus E, Novitsky TJ, Fletcher MA, McKenna TM, and Williams T (1990). Endotoxin binding and neutralizing activity by a protein from Limulus polyphemus. In: Levin J, Alving CR, Munford RS, and Stutz PL, editors. Cellular and molecular aspects of endotoxin reactions. New York: Elsevier Science, 315-325.
186. Wang S, Miura M, Jung YK, Zhu H, Li E, and Yuan J (1998). Murine caspase-11, an ICE-interacting protease, is essential for the activation of ICE. Cell 92:501-509.
187. Warren HS, Glennon ML, Wainwright N, Amato SF, Black KM, Kirsch SJ, Riveau GR, Whyte RI, Zapol WM, and Novitsky TJ (1992). Binding and neutralization of endotoxin by Limulus antilipopolysaccharide factor. Infect Immun 60: 2506-2513.
188. Warren HS, Novitsky TJ, Bucklin A, Kania SA, and Siber GR (1987). Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other gram-negative bacteria. Infect Immun 55:1668-1673.
189. Weinstein SL, Gold MR, and DeFranco AL (1991). Bacterial lipopolysaccharide stimulates protein tyrosine phosphorylation in macrophages. Proc Natl Acad Sci USA 88:4148-4152.
190. Weinstein SL, June CH, and DeFranco AL (1993). Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophages is mediated by CD14. J Immunol 151:3829-3838.
191. Weinstein SL, Sanghera JS, Lemke K, DeFranco AL, and Pelech SL (1992). Bacterial lipopolysaccharide induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in macrophages. J Biol Chem 267:14955-14962.
192. Winn R, Maunder R, Chi E, and Harlan J (1987). Neutrophil depletion does not prevent lung edema after endotoxin infusion in goats. J Appl Physiol 62:116-121.
193. Woods A and Couchman JR (1988). Focal adhesions and cell-matrix interactions. Coll Relat Res 8:155-182.
194. Wright SD, Ramos RA, Hermanowski-Vosatka A, Rockwell P, and Detmers PA (1991). Activation of the adhesive capacity of CR3 on neutrophils by endotoxin: Dependence on lipopolysaccharide binding protein and CD14. J Exp Med 173: 1281-1286.
195. Wysolmerski R, Lagunoff D, and Dahms T (1984). Ethchlorvynol-induced pulmonary edema in rats: An ultrastructural study. Am J Pathol 115:447-457.
196. Yang RB, Mark MR, Gray A, Huang A, Xie MH, Zhang M, Goddard A, Wood WI, Gurney AL, and Godowski PJ (1998). Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling. Nature 395:284-288.
197. Yang Z, Breider MA, Carroll RC, Miller MS, and Bochsler PN (1996). Soluble CD14 and lipopolysaccharide-binding protein from bovine serum enable bacterial lipopolysaccharide-mediated cytotoxicity and activation of bovine vascular endothelial cells in vitro. J Leukoc Biol 59:241-247.
198. Yee J and Christou NV (1993). Neutrophil priming by lipopolysaccharide involves heterogeneity in calcium-mediated signal transduction: Studies using fluo-3 and flow cytometry. J Immunol 150:1988-1997.
199. Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E, Mantovani A, Rothe M, Muzio M, and Arditi M (1999). Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 274:7611-7614.
200. Ziegler EJ, McCutchan JA, Fierer J, Glauser MP, Sadoff JC, Douglas H, and Braude AI (1982). Treatment of gram-negative bacteremia and shock with human antiserum to a mutant Escherichia coli. N Engl J Med 307:1225-1230.