Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories

Metabolic Engineering

Volumn 51, Issue , 2019, Pages 70-78

  Dai, Zhubo ^a,b   Liu, Yun ^a,b   Sun, Zhoutong ^a   Wang, Dong ^a,b   Qu, Ge ^a   Ma, Xiaolin ^a,c   Fan, Feiyu ^a,b   Zhang, Lili ^a,c   Li, Shoulian ^a,c   Zhang, Xueli ^a,b  

^a Chinese Academy of Sciences   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Corosolic acid; Maslinic acid; Saccharomyces cerevisiae; Triterpenoids; Yeast cell factories

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CATALYSIS; ENZYMES; HYDROXYLATION; METABOLIC ENGINEERING; METABOLISM; METABOLITES; YEAST;

BIOSYNTHESIS PATHWAYS; CYTOCHROME P450 ENZYMES; MASLINIC ACIDS; MICROBIAL PRODUCTION; PENTACYCLIC TRITERPENOIDS; PLANT SECONDARY METABOLITES; TRITERPENOIDS; YEAST CELL;

C (PROGRAMMING LANGUAGE);

BETULIC ACID; COMPLEMENTARY DNA; CYTOCHROME P450; MASLINIC ACID; MULTIFUNCTIONAL ENZYME; OLEANOLIC ACID; PENTACYCLIC TRITERPENE; RIBOSOME DNA; TRANSCRIPTOME; TRITERPENOID; ALPHITOLIC ACID; COROSOLIC ACID; TRITERPENE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CODON; CONTROLLED STUDY; CRATAEGUS PINNATIFIDA; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME ASSAY; FED BATCH FERMENTATION; HYDROXYLATION; IN VITRO STUDY; LEAF TISSUE; METABOLIC ENGINEERING; NONHUMAN; OXIDATION; PHYLOGENY; PRIORITY JOURNAL; PROMOTER REGION; SUSPENSION CELL CULTURE; TRANSCRIPTOMICS; YEAST CELL; BIOREACTOR; CRATAEGUS; ENZYMOLOGY; GENETICS; METABOLISM; PLASMID; SACCHAROMYCES CEREVISIAE;

BIOREACTORS; CATALYSIS; CRATAEGUS; CYTOCHROME P-450 ENZYME SYSTEM; HYDROXYLATION; METABOLIC ENGINEERING; PLASMIDS; SACCHAROMYCES CEREVISIAE; TRITERPENES;

EID: 85055650603     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2018.10.001     Document Type: Article

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