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Becker, J., Wittmann, C., Systems and synthetic metabolic engineering for amino acid production – the heartbeat of industrial strain development. Curr Opin Biotechnol 23 (2012), 718–726.
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Becker, J., Wittmann, C., Bio-based production of chemicals, materials and fuels – Corynebacterium glutamicum as versatile cell factory. Curr Opin Biotechnol 23 (2012), 631–640.
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Becker, J., Lange, A., Fabarius, J., Wittmann, C., Top value platform chemicals: bio-based production of organic acids. Curr Opin Biotechnol 36 (2015), 168–175.
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Becker, J., Wittmann, C., Systems metabolic engineering of Escherichia coli for the heterologous production of high value molecules – a veteran at new shores. Curr Opin Biotechnol 42 (2016), 178–188.
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Becker, J., Zelder, O., Häfner, S., Schröder, H., Wittmann, C., From zero to hero – design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab Eng 13 (2011), 159–168.
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13C metabolic flux analysis for the cancer biologist. Exp Mol Med, 50, 2018, 19.
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13C metabolic flux analysis in complex systems. Curr Opin Biotechnol 22 (2011), 103–108.
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Kohlstedt, M., Becker, J., Wittmann, C., Metabolic fluxes and beyond-systems biology understanding and engineering of microbial metabolism. Appl Microbiol Biotechnol 88 (2010), 1065–1075.
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13C labeling enrichment in microbial culture applying metabolic tracer experiments using gas chromatography–combustion–isotope ratio mass spectrometry. Anal Biochem 380 (2008), 202–210.
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Fazenda, M.L., Dias, J.M., Harvey, L.M., Nordon, A., Edrada-Ebel, R., LittleJohn, D., McNeil, B., Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris. Microb Cell Fact, 12, 2013, 51.
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13C tracer strategy provided impressive details on the interplay of different bacterial strains to the process.
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Here, the role of acetic acid bacteria in the cocoa pulp fermentation was investigated. A functionally separated metabolism relies on the fine-tuned ratio of different microbes contributing to the process.
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Adler, P., Frey, L.J., Berger, A., Bolten, C.J., Hansen, C.E., Wittmann, C., The key to acetate: metabolic fluxes of acetic acid bacteria under cocoa pulp fermentation-simulating conditions. Appl Environ Microbiol 80 (2014), 4702–4716 Here, the role of acetic acid bacteria in the cocoa pulp fermentation was investigated. A functionally separated metabolism relies on the fine-tuned ratio of different microbes contributing to the process.
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For the first time, the fluxome of several IgG-producing CHO cell lines was analyzed and compared. Upregulated mitochondrial metabolism and lactate consumption instead of secretion are associated with the producing phenotype.
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13C tracer experiments and a combination of GC–MS, LC–MS, and NMR provide impressive details into riboflavin production with Ashbya gossypii under industrial conditions. Based on the obtained data, tailor-made process optimization resulted in a 45 % increased riboflavin titer.
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13C tracer experiments and a combination of GC–MS, LC–MS, and NMR provide impressive details into riboflavin production with Ashbya gossypii under industrial conditions. Based on the obtained data, tailor-made process optimization resulted in a 45 % increased riboflavin titer.
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13C metabolic flux analysis. Identification of an unknown fructokinase resulted in a succinate producer with improved production performance.
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13C metabolic flux analysis. Identification of an unknown fructokinase resulted in a succinate producer with improved production performance.
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13C metabolic flux analysis: optimal design of isotopic labeling experiments. Curr Opin Biotechnol 24 (2013), 1116–1121.
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Dersch, L.M., Beckers, V., Wittmann, C., Green pathways: metabolic network analysis of plant systems. Metab Eng 34 (2016), 1–24.
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13C metabolic flux analysis, the authors demonstrated for the first time that carbon is lost in the pentose phosphate pathway of mammalian cells, not only in the oxidative branch, but also due to the action of the transketolase-like protein 1.
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13C metabolic flux analysis, the authors demonstrated for the first time that carbon is lost in the pentose phosphate pathway of mammalian cells, not only in the oxidative branch, but also due to the action of the transketolase-like protein 1.
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