메뉴 건너뛰기




Volumn 43, Issue , 2017, Pages 104-109

Recent advances in high-throughput 13C-fluxomics

Author keywords

[No Author keywords available]

Indexed keywords

DATA HANDLING; STRAIN; THROUGHPUT;

EID: 84994881318     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2016.10.010     Document Type: Review
Times cited : (60)

References (70)
  • 1
    • 84856502778 scopus 로고    scopus 로고
    • Metabolomics-assisted synthetic biology
    • 1 Ellis, D.I., Goodacre, R., Metabolomics-assisted synthetic biology. Curr Opin Biotechnol 23 (2012), 22–28.
    • (2012) Curr Opin Biotechnol , vol.23 , pp. 22-28
    • Ellis, D.I.1    Goodacre, R.2
  • 3
    • 0036273832 scopus 로고    scopus 로고
    • Genomics to fluxomics and physiomics — pathway engineering
    • 3 Sanford, K., Soucaille, P., Whited, G., Chotani, G., Genomics to fluxomics and physiomics — pathway engineering. Curr Opin Microbiol 5 (2002), 318–322.
    • (2002) Curr Opin Microbiol , vol.5 , pp. 318-322
    • Sanford, K.1    Soucaille, P.2    Whited, G.3    Chotani, G.4
  • 4
    • 84902332213 scopus 로고    scopus 로고
    • Quantitative flux analysis reveals folate-dependent NADPH production
    • 4 Fan, J., Ye, J., Kamphorst, J.J., Shlomi, T., Thompson, C.B., Rabinowitz, J.D., Quantitative flux analysis reveals folate-dependent NADPH production. Nature 510 (2014), 298–302.
    • (2014) Nature , vol.510 , pp. 298-302
    • Fan, J.1    Ye, J.2    Kamphorst, J.J.3    Shlomi, T.4    Thompson, C.B.5    Rabinowitz, J.D.6
  • 6
    • 84877296949 scopus 로고    scopus 로고
    • Systematic identification of allosteric protein–metabolite interactions that control enzyme activity in vivo
    • 6 Link, H., Kochanowski, K., Sauer, U., Systematic identification of allosteric protein–metabolite interactions that control enzyme activity in vivo. Nat Biotechnol 31 (2013), 357–361.
    • (2013) Nat Biotechnol , vol.31 , pp. 357-361
    • Link, H.1    Kochanowski, K.2    Sauer, U.3
  • 7
    • 12244308282 scopus 로고    scopus 로고
    • 13C experiments for metabolic variant discrimination
    • 13C experiments for metabolic variant discrimination. Genome Biol, 5, 2004, R99.
    • (2004) Genome Biol , vol.5 , pp. R99
    • Zamboni, N.1    Sauer, U.2
  • 11
    • 80755159333 scopus 로고    scopus 로고
    • Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1
    • 11 Peyraud, R., Schneider, K., Kiefer, P., Massou, S., Vorholt, J.A., Portais, J.C., Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1. BMC Syst Biol, 5, 2011, 189.
    • (2011) BMC Syst Biol , vol.5 , pp. 189
    • Peyraud, R.1    Schneider, K.2    Kiefer, P.3    Massou, S.4    Vorholt, J.A.5    Portais, J.C.6
  • 12
    • 79952106791 scopus 로고    scopus 로고
    • From zero to hero — design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production
    • 12 Becker, J., Zelder, O., Hafner, S., Schroder, H., Wittmann, C., From zero to hero — design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab Eng 13 (2011), 159–168.
    • (2011) Metab Eng , vol.13 , pp. 159-168
    • Becker, J.1    Zelder, O.2    Hafner, S.3    Schroder, H.4    Wittmann, C.5
  • 14
    • 50349092706 scopus 로고    scopus 로고
    • Kinetic flux profiling for quantitation of cellular metabolic fluxes
    • 14 Yuan, J., Bennett, B.D., Rabinowitz, J.D., Kinetic flux profiling for quantitation of cellular metabolic fluxes. Nat Protoc 3 (2008), 1328–1340.
    • (2008) Nat Protoc , vol.3 , pp. 1328-1340
    • Yuan, J.1    Bennett, B.D.2    Rabinowitz, J.D.3
  • 15
    • 84887626505 scopus 로고    scopus 로고
    • Isotopically non-stationary metabolic flux analysis: complex yet highly informative
    • 15 Wiechert, W., Noh, K., Isotopically non-stationary metabolic flux analysis: complex yet highly informative. Curr Opin Biotechnol 24 (2013), 979–986.
    • (2013) Curr Opin Biotechnol , vol.24 , pp. 979-986
    • Wiechert, W.1    Noh, K.2
  • 16
    • 3042731363 scopus 로고    scopus 로고
    • Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale
    • 16 Wittmann, C., Kim, H.M., Heinzle, E., Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale. Biotechnol Bioeng 87 (2004), 1–6.
    • (2004) Biotechnol Bioeng , vol.87 , pp. 1-6
    • Wittmann, C.1    Kim, H.M.2    Heinzle, E.3
  • 17
    • 78649832864 scopus 로고    scopus 로고
    • Unraveling condition-dependent networks of transcription factors that control metabolic pathway activity in yeast
    • 13C-fluxomics to quantify intracellular fluxes in 119 transcription factor deletion mutants of Saccharomyces cerevisiae grown under five conditions. Fluxes are integrated with transcript and protein levels to explore the transcriptional control of yeast metabolism.
    • 13C-fluxomics to quantify intracellular fluxes in 119 transcription factor deletion mutants of Saccharomyces cerevisiae grown under five conditions. Fluxes are integrated with transcript and protein levels to explore the transcriptional control of yeast metabolism.
    • (2010) Mol Syst Biol , vol.6 , pp. 432
    • Fendt, S.M.1    Oliveira, A.P.2    Christen, S.3    Picotti, P.4    Dechant, R.C.5    Sauer, U.6
  • 18
    • 0037274223 scopus 로고    scopus 로고
    • Metabolic screening of mammalian cell cultures using well-plates
    • 18 Balcarcel, R.R., Clark, L.M., Metabolic screening of mammalian cell cultures using well-plates. Biotechnol Prog 19 (2003), 98–108.
    • (2003) Biotechnol Prog , vol.19 , pp. 98-108
    • Balcarcel, R.R.1    Clark, L.M.2
  • 20
    • 84914117921 scopus 로고    scopus 로고
    • The development and application of high throughput cultivation technology in bioprocess development
    • 20 Long, Q., Liu, X., Yang, Y., Li, L., Harvey, L., McNeil, B., Bai, Z., The development and application of high throughput cultivation technology in bioprocess development. J Biotechnol 192:Pt B (2014), 323–338.
    • (2014) J Biotechnol , vol.192 , pp. 323-338
    • Long, Q.1    Liu, X.2    Yang, Y.3    Li, L.4    Harvey, L.5    McNeil, B.6    Bai, Z.7
  • 21
    • 79851490029 scopus 로고    scopus 로고
    • A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need
    • 21 Bareither, R., Pollard, D., A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need. Biotechnol Prog 27 (2011), 2–14.
    • (2011) Biotechnol Prog , vol.27 , pp. 2-14
    • Bareither, R.1    Pollard, D.2
  • 22
    • 84938778207 scopus 로고    scopus 로고
    • Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors
    • 22 Wewetzer, S.J., Kunze, M., Ladner, T., Luchterhand, B., Roth, S., Rahmen, N., Kloss, R., Costa, E.S.A., Regestein, L., Buchs, J., Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors. J Biol Eng, 9, 2015, 9.
    • (2015) J Biol Eng , vol.9 , pp. 9
    • Wewetzer, S.J.1    Kunze, M.2    Ladner, T.3    Luchterhand, B.4    Roth, S.5    Rahmen, N.6    Kloss, R.7    Costa, E.S.A.8    Regestein, L.9    Buchs, J.10
  • 23
    • 84934294281 scopus 로고    scopus 로고
    • A novel milliliter-scale chemostat system for parallel cultivation of microorganisms in stirred-tank bioreactors
    • 23 Schmideder, A., Severin, T.S., Cremer, J.H., Weuster-Botz, D., A novel milliliter-scale chemostat system for parallel cultivation of microorganisms in stirred-tank bioreactors. J Biotechnol 210 (2015), 19–24.
    • (2015) J Biotechnol , vol.210 , pp. 19-24
    • Schmideder, A.1    Severin, T.S.2    Cremer, J.H.3    Weuster-Botz, D.4
  • 24
    • 84908486152 scopus 로고    scopus 로고
    • Feeding strategies enhance high cell density cultivation and protein expression in milliliter scale bioreactors
    • 24 Faust, G., Janzen, N.H., Bendig, C., Romer, L., Kaufmann, K., Weuster-Botz, D., Feeding strategies enhance high cell density cultivation and protein expression in milliliter scale bioreactors. Biotechnol J 9 (2014), 1293–1303.
    • (2014) Biotechnol J , vol.9 , pp. 1293-1303
    • Faust, G.1    Janzen, N.H.2    Bendig, C.3    Romer, L.4    Kaufmann, K.5    Weuster-Botz, D.6
  • 25
    • 84928727000 scopus 로고    scopus 로고
    • Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping
    • 25 Unthan, S., Radek, A., Wiechert, W., Oldiges, M., Noack, S., Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping. Microb Cell Fact, 14, 2015, 32.
    • (2015) Microb Cell Fact , vol.14 , pp. 32
    • Unthan, S.1    Radek, A.2    Wiechert, W.3    Oldiges, M.4    Noack, S.5
  • 26
    • 84994862268 scopus 로고    scopus 로고
    • Automated sample preparation
    • 26 Bay, M., Automated sample preparation. Science, 2016.
    • (2016) Science
    • Bay, M.1
  • 27
    • 33847691476 scopus 로고    scopus 로고
    • Fluxome analysis using GC–MS
    • 27 Wittmann, C., Fluxome analysis using GC–MS. Microb Cell Fact, 6, 2007, 6.
    • (2007) Microb Cell Fact , vol.6 , pp. 6
    • Wittmann, C.1
  • 29
    • 84973335042 scopus 로고    scopus 로고
    • Characterization of physiological responses to 22 gene knockouts in Escherichia coli central carbon metabolism
    • 29 Long, C.P., Gonzalez, J.E., Sandoval, N.R., Antoniewicz, M.R., Characterization of physiological responses to 22 gene knockouts in Escherichia coli central carbon metabolism. Metab Eng 37 (2016), 102–113.
    • (2016) Metab Eng , vol.37 , pp. 102-113
    • Long, C.P.1    Gonzalez, J.E.2    Sandoval, N.R.3    Antoniewicz, M.R.4
  • 30
    • 84921833977 scopus 로고    scopus 로고
    • 13C metabolic flux analysis
    • 13C labelled experiment to computational flux calculation — the different steps of the procedure for flux determination based on the quantification of isotopologue distributions by GC–MS.
    • 13C labelled experiment to computational flux calculation — the different steps of the procedure for flux determination based on the quantification of isotopologue distributions by GC–MS.
    • (2014) Methods Mol Biol , vol.1191 , pp. 165-174
    • Becker, J.1    Wittmann, C.2
  • 31
    • 84946476393 scopus 로고    scopus 로고
    • Real-time metabolome profiling of the metabolic switch between starvation and growth
    • 31 Link, H., Fuhrer, T., Gerosa, L., Zamboni, N., Sauer, U., Real-time metabolome profiling of the metabolic switch between starvation and growth. Nat Methods 12 (2015), 1091–1097.
    • (2015) Nat Methods , vol.12 , pp. 1091-1097
    • Link, H.1    Fuhrer, T.2    Gerosa, L.3    Zamboni, N.4    Sauer, U.5
  • 32
    • 84908577185 scopus 로고    scopus 로고
    • Direct analysis of intact proteins from Escherichia coli colonies by liquid extraction surface analysis mass spectrometry
    • 32 Randall, E.C., Bunch, J., Cooper, H.J., Direct analysis of intact proteins from Escherichia coli colonies by liquid extraction surface analysis mass spectrometry. Anal Chem 86 (2014), 10504–10510.
    • (2014) Anal Chem , vol.86 , pp. 10504-10510
    • Randall, E.C.1    Bunch, J.2    Cooper, H.J.3
  • 33
    • 84958981814 scopus 로고    scopus 로고
    • Applications of NMR spectroscopy to systems biochemistry
    • This comprehensive review on NMR for metabolic studies describes the current NMR experiments available for isotopomer analysis.
    • 33•• Fan, T.W.M., Lane, A.N., Applications of NMR spectroscopy to systems biochemistry. Progr Nuclear Magn Reson Spectr 92-93 (2016), 18–53 This comprehensive review on NMR for metabolic studies describes the current NMR experiments available for isotopomer analysis.
    • (2016) Progr Nuclear Magn Reson Spectr , vol.92-93 , pp. 18-53
    • Fan, T.W.M.1    Lane, A.N.2
  • 35
    • 84988038669 scopus 로고    scopus 로고
    • Multidimensional NMR approaches towards highly resolved, sensitive and high-throughput quantitative metabolomics
    • 35 Marchand, J., Martineau, E., Guitton, Y., Dervilly-Pinel, G., Giraudeau, P., Multidimensional NMR approaches towards highly resolved, sensitive and high-throughput quantitative metabolomics. Curr Opin Biotechnol 43 (2016), 49–55.
    • (2016) Curr Opin Biotechnol , vol.43 , pp. 49-55
    • Marchand, J.1    Martineau, E.2    Guitton, Y.3    Dervilly-Pinel, G.4    Giraudeau, P.5
  • 36
    • 34447502083 scopus 로고    scopus 로고
    • 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles
    • 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles. Methods Mol Biol 358 (2007), 177–197.
    • (2007) Methods Mol Biol , vol.358 , pp. 177-197
    • Nanchen, A.1    Fuhrer, T.2    Sauer, U.3
  • 37
    • 84869469815 scopus 로고    scopus 로고
    • Tandem mass spectrometry: a new platform for fluxomics
    • 37 Peng, L., Tandem mass spectrometry: a new platform for fluxomics. J Proteomics Bioinform, 2012, 5.
    • (2012) J Proteomics Bioinform , pp. 5
    • Peng, L.1
  • 38
    • 84910625122 scopus 로고    scopus 로고
    • Isotopic studies of metabolic systems by mass spectrometry: using Pascal's triangle to produce biological standards with fully controlled labeling patterns
    • 38 Millard, P., Massou, S., Portais, J.C., Letisse, F., Isotopic studies of metabolic systems by mass spectrometry: using Pascal's triangle to produce biological standards with fully controlled labeling patterns. Anal Chem 86 (2014), 10288–10295.
    • (2014) Anal Chem , vol.86 , pp. 10288-10295
    • Millard, P.1    Massou, S.2    Portais, J.C.3    Letisse, F.4
  • 39
    • 67650479798 scopus 로고    scopus 로고
    • Increasing the accuracy of mass isotopomer analysis through calibration curves constructed using biologically synthesized compounds
    • 39 Shen, T., Shen, W., Xiong, Y., Liu, H., Zheng, H., Zhou, H., Rui, B., Liu, J., Wu, J., Shi, Y., Increasing the accuracy of mass isotopomer analysis through calibration curves constructed using biologically synthesized compounds. J Mass Spectrom 44 (2009), 1066–1080.
    • (2009) J Mass Spectrom , vol.44 , pp. 1066-1080
    • Shen, T.1    Shen, W.2    Xiong, Y.3    Liu, H.4    Zheng, H.5    Zhou, H.6    Rui, B.7    Liu, J.8    Wu, J.9    Shi, Y.10
  • 40
    • 84875595569 scopus 로고    scopus 로고
    • eMZed: an open source framework in Python for rapid and interactive development of LC/MS data analysis workflows
    • 40 Kiefer, P., Schmitt, U., Vorholt, J.A., eMZed: an open source framework in Python for rapid and interactive development of LC/MS data analysis workflows. Bioinformatics 29 (2013), 963–964.
    • (2013) Bioinformatics , vol.29 , pp. 963-964
    • Kiefer, P.1    Schmitt, U.2    Vorholt, J.A.3
  • 41
    • 84860498026 scopus 로고    scopus 로고
    • IsoCor: correcting MS data in isotope labeling experiments
    • 41 Millard, P., Letisse, F., Sokol, S., Portais, J.C., IsoCor: correcting MS data in isotope labeling experiments. Bioinformatics 28 (2012), 1294–1296.
    • (2012) Bioinformatics , vol.28 , pp. 1294-1296
    • Millard, P.1    Letisse, F.2    Sokol, S.3    Portais, J.C.4
  • 43
    • 84902513043 scopus 로고    scopus 로고
    • 13C-metabolic flux analysis of the extremophile Thermus thermophilus HB8
    • 13C-metabolic flux analysis of the extremophile Thermus thermophilus HB8. Metab Eng 24 (2014), 173–180.
    • (2014) Metab Eng , vol.24 , pp. 173-180
    • Swarup, A.1    Lu, J.2    DeWoody, K.C.3    Antoniewicz, M.R.4
  • 46
    • 84899450145 scopus 로고    scopus 로고
    • Robustness and plasticity of metabolic pathway flux among uropathogenic isolates of Pseudomonas aeruginosa
    • 46 Berger, A., Dohnt, K., Tielen, P., Jahn, D., Becker, J., Wittmann, C., Robustness and plasticity of metabolic pathway flux among uropathogenic isolates of Pseudomonas aeruginosa. PLoS One, 9, 2014, e88368.
    • (2014) PLoS One , vol.9 , pp. e88368
    • Berger, A.1    Dohnt, K.2    Tielen, P.3    Jahn, D.4    Becker, J.5    Wittmann, C.6
  • 49
    • 33745155105 scopus 로고    scopus 로고
    • Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements
    • 49 Antoniewicz, M.R., Kelleher, J.K., Stephanopoulos, G., Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements. Metab Eng 8 (2006), 324–337.
    • (2006) Metab Eng , vol.8 , pp. 324-337
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 53
    • 84857845281 scopus 로고    scopus 로고
    • influx_s: increasing numerical stability and precision for metabolic flux analysis in isotope labelling experiments
    • Similar to Yang et al. (2007) and Mottelet et al. (2016), this article describes how novel optimization algorithms may improve both the accuracy and speed of metabolic flux calculations.
    • 53• Sokol, S., Millard, P., Portais, J.C., influx_s: increasing numerical stability and precision for metabolic flux analysis in isotope labelling experiments. Bioinformatics 28 (2012), 687–693 Similar to Yang et al. (2007) and Mottelet et al. (2016), this article describes how novel optimization algorithms may improve both the accuracy and speed of metabolic flux calculations.
    • (2012) Bioinformatics , vol.28 , pp. 687-693
    • Sokol, S.1    Millard, P.2    Portais, J.C.3
  • 55
    • 84994834380 scopus 로고    scopus 로고
    • Metabolic flux analysis in isotope labeling experiments using the adjoint approach
    • 55 Mottelet, S., Gaullier, G., Sadaka, G., Metabolic flux analysis in isotope labeling experiments using the adjoint approach. IEEE/ACM Trans Comput Biol Bioinform, 2016, 10.1109/TCBB.2016.2542802.
    • (2016) IEEE/ACM Trans Comput Biol Bioinform
    • Mottelet, S.1    Gaullier, G.2    Sadaka, G.3
  • 56
    • 51049091904 scopus 로고    scopus 로고
    • Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell line
    • 56 Yoo, H., Antoniewicz, M.R., Stephanopoulos, G., Kelleher, J.K., Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell line. J Biol Chem 283 (2008), 20621–20627.
    • (2008) J Biol Chem , vol.283 , pp. 20621-20627
    • Yoo, H.1    Antoniewicz, M.R.2    Stephanopoulos, G.3    Kelleher, J.K.4
  • 57
    • 0343267841 scopus 로고    scopus 로고
    • Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems
    • 57 Wiechert, W., Mollney, M., Isermann, N., Wurzel, M., de Graaf, A.A., Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems. Biotechnol Bioeng 66 (1999), 69–85.
    • (1999) Biotechnol Bioeng , vol.66 , pp. 69-85
    • Wiechert, W.1    Mollney, M.2    Isermann, N.3    Wurzel, M.4    de Graaf, A.A.5
  • 58
    • 0036271587 scopus 로고    scopus 로고
    • Analysis of flux estimates based on 13C-labelling experiments
    • 58 Christensen, B., Gombert, A.K., Nielsen, J., Analysis of flux estimates based on 13C-labelling experiments. Eur J Biochem 269 (2002), 2795–2800.
    • (2002) Eur J Biochem , vol.269 , pp. 2795-2800
    • Christensen, B.1    Gombert, A.K.2    Nielsen, J.3
  • 59
    • 33845679072 scopus 로고    scopus 로고
    • Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions
    • 59 Antoniewicz, M.R., Kelleher, J.K., Stephanopoulos, G., Elementary metabolite units (EMU): a novel framework for modeling isotopic distributions. Metab Eng 9 (2007), 68–86.
    • (2007) Metab Eng , vol.9 , pp. 68-86
    • Antoniewicz, M.R.1    Kelleher, J.K.2    Stephanopoulos, G.3
  • 61
    • 84941966264 scopus 로고    scopus 로고
    • Efficient modeling of MS/MS data for metabolic flux analysis
    • 61 Tepper, N., Shlomi, T., Efficient modeling of MS/MS data for metabolic flux analysis. PLoS One, 10, 2015, e0130213.
    • (2015) PLoS One , vol.10 , pp. e0130213
    • Tepper, N.1    Shlomi, T.2
  • 63
    • 84942248861 scopus 로고    scopus 로고
    • 13C metabolic flux analysis at a genome-scale
    • 13C-fluxomics on compartmentalized eukaryotic systems such as yeast. They point out the current bottlenecks for improving genome-scale metabolic flux analysis.
    • 13C-fluxomics on compartmentalized eukaryotic systems such as yeast. They point out the current bottlenecks for improving genome-scale metabolic flux analysis.
    • (2015) Metab Eng , vol.32 , pp. 12-22
    • Gopalakrishnan, S.1    Maranas, C.D.2
  • 67
    • 79960342167 scopus 로고    scopus 로고
    • Visualizing multi-omics data in metabolic networks with the software Omix: a case study
    • 67 Droste, P., Miebach, S., Niedenfuhr, S., Wiechert, W., Noh, K., Visualizing multi-omics data in metabolic networks with the software Omix: a case study. Biosystems 105 (2011), 154–161.
    • (2011) Biosystems , vol.105 , pp. 154-161
    • Droste, P.1    Miebach, S.2    Niedenfuhr, S.3    Wiechert, W.4    Noh, K.5
  • 68
    • 84919338721 scopus 로고    scopus 로고
    • 13C fluxomics
    • This review establishes a unified taxonomy to compare the various fluxomics methods. Challenges and opportunities of current modelling approaches are discussed.
    • 13C fluxomics. Curr Opin Biotechnol 34 (2015), 82–90 This review establishes a unified taxonomy to compare the various fluxomics methods. Challenges and opportunities of current modelling approaches are discussed.
    • (2015) Curr Opin Biotechnol , vol.34 , pp. 82-90
    • Niedenfuhr, S.1    Wiechert, W.2    Noh, K.3
  • 70
    • 84994908000 scopus 로고    scopus 로고
    • 13C metabolic flux analysis
    • A founding paper that establishes the standard of integrated fluxomics workflows. All aspects of the experimental workflow were considered. This includes not only all the computational and mathematical steps of fluxomics, but also the management of data, metadata, models, versioning, among others, advanced visualization tools, and a complete, service-oriented interface that complies with modern standards.
    • 13C metabolic flux analysis. J Biotechnol 232 (2016), 12–24 A founding paper that establishes the standard of integrated fluxomics workflows. All aspects of the experimental workflow were considered. This includes not only all the computational and mathematical steps of fluxomics, but also the management of data, metadata, models, versioning, among others, advanced visualization tools, and a complete, service-oriented interface that complies with modern standards.
    • (2016) J Biotechnol , vol.232 , pp. 12-24
    • Dalman, T.1    Wiechert, W.2    Noh, K.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.