CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Science

Volumn 360, Issue 6387, 2018, Pages 436-439

  Chen, Janice S ^a   Ma, Enbo ^a   Harrington, Lucas B ^a   Da Costa, Maria ^b   Tian, Xinran ^a   Palefsky, Joel M ^b   Doudna, Jennifer A ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN CAS12A; DOUBLE STRANDED DNA; RNA; SINGLE STRANDED DNA; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CPF1 NUCLEASE, ACIDAMINOCOCCUS; ENDONUCLEASE;

DNA; ENZYME ACTIVITY; GENE EXPRESSION; GENOME; IMMUNE SYSTEM; MOLECULAR ANALYSIS;

ACIDAMINOCOCCUS; ALICYCLOBACILLUS; ALICYCLOBACILLUS ACIDOTERRESTRIS; ANALYTIC METHOD; ARTICLE; DNA BINDING; DNA DETERMINATION; DNA ENDONUCLEASE TARGETED CRISPR TRANS REPORTER; ENZYME ACTIVATION; ENZYME ACTIVITY; FRANCISELLA NOVICIDA; GENE AMPLIFICATION; HUMAN; HUMAN PAPILLOMAVIRUS TYPE 16; HUMAN PAPILLOMAVIRUS TYPE 18; LACHNOSPIRACEAE; MOLECULAR DIAGNOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; VIRUS DETECTION; CHEMISTRY; CLOSTRIDIALES; CRISPR CAS SYSTEM; DNA CLEAVAGE; ENZYME SPECIFICITY; ENZYMOLOGY; KINETICS;

BACTERIA (MICROORGANISMS); HUMAN PAPILLOMAVIRUS;

BACTERIAL PROTEINS; CLOSTRIDIALES; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DNA CLEAVAGE; DNA, SINGLE-STRANDED; ENDONUCLEASES; KINETICS; SUBSTRATE SPECIFICITY;

EID: 85042354098     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aar6245     Document Type: Article

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