Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR-Cas9 genome-editing efficiency

Nature Biotechnology

Volumn 36, Issue 1, 2018, Pages 95-102

  Canny, Marella D ^a   Moatti, Nathalie ^a   Wan, Leo C K ^a,b   Fradet Turcotte, Amélie ^a,e   Krasner, Danielle ^c   Mateos Gomez, Pedro A ^d   Zimmermann, Michal ^a   Orthwein, Alexandre ^a,e,f,g   Juang, Yu Chi ^a   Zhang, Wei ^b   Noordermeer, Sylvie M ^a   Seclen, Eduardo ^c   Wilson, Marcus D ^a   Vorobyov, Andrew ^b   Munro, Meagan ^a   Ernst, Andreas ^b,e,h   Ng, Timothy F ^a,b   Cho, Tiffany ^a,b   Cannon, Paula M ^c   Sidhu, Sachdev S ^b   Sicheri, Frank ^a,b   Durocher, Daniel ^a,b   more..

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^d NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e University Laval   (Canada)

^f MCGILL UNIVERSITY   (Canada)

^g MCGILL UNIVERSITY   (Canada)

^h GOETHE UNIVERSITY MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; EFFICIENCY; GENE EXPRESSION; MAMMALS; OLIGONUCLEOTIDES;

COMBINATORIAL LIBRARY; DOUBLE STRAND BREAK REPAIR; DOUBLE STRANDED DNA; EUKARYOTIC CELLS; GENE CONVERSION; NONHOMOLOGOUS END JOINING; RATE-LIMITING STEPS; REPAIR PATHWAYS;

REPAIR;

DNA; DOUBLE STRANDED DNA; I53 PROTEIN; INHIBITOR PROTEIN; PROTEIN VARIANT; SINGLE STRANDED DNA; TUMOR SUPPRESSOR P53 BINDING PROTEIN 1; UBIQUITIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHROMOSOME; COMBINATORIAL LIBRARY; COMPLEX FORMATION; CONTROLLED STUDY; CRISPR-CAS9 SYSTEM; DNA END JOINING REPAIR; DNA REPAIR; EMBRYO; GENE CONVERSION; GENE EDITING; GENE TARGETING; GENETIC TRANSFECTION; HOMOLOGY DEPENDENT REPAIR; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; TUDOR DOMAIN; ANIMAL; ANTAGONISTS AND INHIBITORS; CRISPR CAS SYSTEM; DNA DAMAGE; GENE EXPRESSION REGULATION; GENETICS; RECOMBINATION REPAIR;

ANIMALS; CRISPR-CAS SYSTEMS; DNA DAMAGE; DNA END-JOINING REPAIR; DNA REPAIR; GENE EDITING; GENE EXPRESSION REGULATION; HUMANS; MICE; RECOMBINATIONAL DNA REPAIR; TUMOR SUPPRESSOR P53-BINDING PROTEIN 1;

EID: 85040526330     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.4021     Document Type: Article

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