Construction of a versatile expression library for all human single-pass transmembrane proteins for receptor pairings by high throughput screening

Journal of Biotechnology

Volumn 260, Issue , 2017, Pages 18-30

  Yang, Wei ^a   Padkjær, Søren Berg ^b   Wang, Jishu ^a   Sun, Zhe ^a   Shan, Bing ^a   Yang, Li ^a   Chen, Haibin ^a,c   Kang, Lishan ^a,d   Madsen, Dennis ^b   Li, Xun ^a   Shen, Chenxi ^a   Yu, Bingke ^a   Zhu, Haisun ^a   Chao, Tzu Yuan ^a,e   Cao, Zhuoxiao ^a   Li, Dapeng ^a   Liu, Wei ^a   Du, Yanping ^a   Xu, Jinjing ^a   Hao, Dongxia ^a   Xu, Fengting ^a   Peng, Lujia ^a   Li, Tengkun ^a   Wang, Lin ^a   Li, Lin ^a   Xing, Haimei ^a   Liu, Di ^a   Liu, Zibing ^a   Guan, Zhishuang ^a   Wang, Wan ^a   Cheng, Hong ^a   Østergaard, Henrik ^b   Chang, Chihchuan ^a   Yang, Zhiru ^a   Boel, Esper ^b,f   Su, Jing ^a,g   more..

^a SIBS Novo Nordisk Translational Research Centre for PreDiabetes   (China)

^b NOVO NORDISK A S   (Denmark)

^c Enzymaster (Ningbo) Bio engineering Co Ltd   (China)

^d ChemPartner ^*  (China)

^e Synermore Biologics Co Ltd   (Taiwan)

^f BoelBioMed Consulting   (Denmark)

^g Neurosciences Therapeutic Area Unit   (China)

Author keywords

Expression library; Fab; High throughput screening; IgSF11; VISTA

Indexed keywords

BINS; BIOCHEMISTRY; CELL MEMBRANES; CELLS; CYTOLOGY; FLOW CYTOMETRY; LIGANDS; MAMMALS; PROTEINS; SURFACE PLASMON RESONANCE; T-CELLS; THROUGHPUT;

EXTRACELLULAR DOMAINS; FLUORESCENCE ACTIVATED CELL SORTING; HIGH THROUGHPUT SCREENING; IGSF11; PHYSIOLOGICAL LIGANDS; PROTEIN-PROTEIN BINDING; TRANS-MEMBRANE PROTEINS; VISTA;

CELL SIGNALING;

BLOOD CLOTTING FACTOR 7A; ENDOTHELIAL PROTEIN C RECEPTOR; IMMUNOGLOBULIN; MEMBRANE PROTEIN; THROMBOPLASTIN; CELL SURFACE RECEPTOR; FUSION PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT;

ARTICLE; BINDING ASSAY; FLUORESCENCE ACTIVATED CELL SORTING; HEMOPHILIA; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN BINDING; SURFACE PLASMON RESONANCE; T LYMPHOCYTE ACTIVATION; CHEMISTRY; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; METABOLISM; MOLECULAR CLONING; PEPTIDE LIBRARY;

CLONING, MOLECULAR; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MEMBRANE PROTEINS; PEPTIDE LIBRARY; RECEPTORS, CELL SURFACE; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 85028814278     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2017.08.023     Document Type: Article

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