메뉴 건너뛰기




Volumn 79, Issue 1, 2011, Pages 7-15

Generation and evaluation of mammalian secreted and membrane protein expression libraries for high-throughput target discovery

Author keywords

'Tag after stop codon'; 96 Well mammalian protein expression; HEK293; High throughput; Membrane proteins; Receptor ligand; Secretome

Indexed keywords

MEMBRANE PROTEIN; PEPTIDE LIBRARY; PROTEIN;

EID: 79960148752     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.011     Document Type: Article
Times cited : (3)

References (29)
  • 2
    • 84934436279 scopus 로고    scopus 로고
    • High-Throughput Protein Production (HTPP): A review of enabling technologies to expedite protein production
    • J. Koehn, and I. Hunt High-Throughput Protein Production (HTPP): a review of enabling technologies to expedite protein production Methods Mol. Biol. 498 2009 1 18
    • (2009) Methods Mol. Biol. , vol.498 , pp. 1-18
    • Koehn, J.1    Hunt, I.2
  • 5
    • 27644489022 scopus 로고    scopus 로고
    • International human genome sequencing consortium, finishing the euchromatic sequence of the human genome
    • International human genome sequencing consortium, finishing the euchromatic sequence of the human genome Nature 431 2004 931 945
    • (2004) Nature , vol.431 , pp. 931-945
  • 7
    • 66049131300 scopus 로고    scopus 로고
    • Decrypting the genome's alternative messages
    • B. Hartmann, and J. Valcárcel Decrypting the genome's alternative messages Curr. Opin. Cell Biol. 21 2009 377 386
    • (2009) Curr. Opin. Cell Biol. , vol.21 , pp. 377-386
    • Hartmann, B.1    Valcárcel, J.2
  • 8
    • 84934441135 scopus 로고    scopus 로고
    • E. Coli and insect cell expression, automated purification and quantitative analysis
    • S.P. Chambers, J.R. Fulghum, D.A. Austen, F. Lu, and S.E. Swalley E. Coli and insect cell expression, automated purification and quantitative analysis Methods Mol. Biol. 498 2009 143 156
    • (2009) Methods Mol. Biol. , vol.498 , pp. 143-156
    • Chambers, S.P.1    Fulghum, J.R.2    Austen, D.A.3    Lu, F.4    Swalley, S.E.5
  • 11
    • 44949196798 scopus 로고    scopus 로고
    • Protein structure modeling indicates hexahistidine-tag interference with enzyme activity
    • DOI 10.1002/prot.21905
    • A.C. Freydank, W. Brandt, and B. Dräger Protein structure modeling indicates hexahistidine-tag interference with enzyme activity Proteins 72 2008 173 183 (Pubitemid 351809156)
    • (2008) Proteins: Structure, Function and Genetics , vol.72 , Issue.1 , pp. 173-183
    • Freydank, A.-C.1    Brandt, W.2    Drager, B.3
  • 13
    • 0037082158 scopus 로고    scopus 로고
    • High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
    • Y. Durocher, S. Perret, and A. Kamen High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells Nucleic Acids Res. 30 2002 E9
    • (2002) Nucleic Acids Res. , vol.30 , pp. 9
    • Durocher, Y.1    Perret, S.2    Kamen, A.3
  • 15
    • 0033044637 scopus 로고    scopus 로고
    • Machine learning approaches for the prediction of signal peptides and other protein sorting signals
    • H. Nielsen, S. Brunak, and G. von Heijne Machine learning approaches to the prediction of signal peptides and other protein sorting signals Prot. Eng. 12 1999 3 9 (Pubitemid 29050535)
    • (1999) Protein Engineering , vol.12 , Issue.1 , pp. 3-9
    • Nielsen, H.1    Brunak, S.2    Von Heijne, G.3
  • 16
    • 33646068398 scopus 로고    scopus 로고
    • FuGENE® 6 transfection reagent: Minimizing reagent-dependent side effects as analyzed by gene-expression profiling and cytotoxicity assays
    • 10.1038/nmeth879
    • V. Nagy, and M. Watzele FuGENE® 6 transfection reagent: minimizing reagent-dependent side effects as analyzed by gene-expression profiling and cytotoxicity assays Nature Methods 3 2006 10.1038/nmeth879
    • (2006) Nature Methods , vol.3
    • Nagy, V.1    Watzele, M.2
  • 17
    • 33750536208 scopus 로고    scopus 로고
    • A high-throughput mammalian protein expression, purification, aliquoting and storage pipeline to assemble a library of the human secretome
    • DOI 10.2174/138620706778700143
    • T. Battle, B. Antonsson, G. Feger, and D. Besson A high-throughput mammalian protein expression purification, aliquoting and storage pipeline to assemble a library of the human secretome. Comb. Chem. High Throughput Screen. 9 2006 639 649 (Pubitemid 44674229)
    • (2006) Combinatorial Chemistry and High Throughput Screening , vol.9 , Issue.9 , pp. 639-649
    • Battle, T.1    Antonsson, B.2    Feger, G.3    Besson, D.4
  • 18
    • 20444399919 scopus 로고    scopus 로고
    • Optimisation and evaluation of a high-throughput mammalian protein expression system
    • DOI 10.1016/j.pep.2005.03.012, PII S1046592805000926
    • A. Davies, A. Greene, E. Lullau, and W.M. Abbott Optimisation and evaluation of a high-throughput mammalian protein expression system Protein Expr. Purif. 42 2005 111 121 (Pubitemid 40793400)
    • (2005) Protein Expression and Purification , vol.42 , Issue.1 , pp. 111-121
    • Davies, A.1    Greene, A.2    Lullau, E.3    Abbott, W.M.4
  • 19
    • 0021909735 scopus 로고
    • Amber, ochre and opal suppressor trna genes derived from a human serine tRNA gene
    • J.P. Capone, P.A. Sharp, and U.L. RajBhandary Amber, ochre and opal suppressor trna genes derived from a human serine tRNA gene EMBO J. 4 1985 213 221
    • (1985) EMBO J. , vol.4 , pp. 213-221
    • Capone, J.P.1    Sharp, P.A.2    Rajbhandary, U.L.3
  • 20
    • 0034002603 scopus 로고    scopus 로고
    • Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes
    • DOI 10.1128/MCB.20.9.3116-3124.2000
    • M. Buvoli, A. Buvoli, and L.A. Leinwand Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes Mol. Cell. Biol. 20 2000 3116 3124 (Pubitemid 30215024)
    • (2000) Molecular and Cellular Biology , vol.20 , Issue.9 , pp. 3116-3124
    • Buvoli, M.1    Buvoli, A.2    Leinwand, L.A.3
  • 23
    • 71749092103 scopus 로고    scopus 로고
    • Unraveling the structure and function of G protein-coupled receptors through NMR spectroscopy
    • I.G. Tikhonova, and S. Costanzi Unraveling the structure and function of G protein-coupled receptors through NMR spectroscopy Curr. Pharm. Des. 15 2009 4003 4016
    • (2009) Curr. Pharm. Des. , vol.15 , pp. 4003-4016
    • Tikhonova, I.G.1    Costanzi, S.2
  • 25
    • 84860419274 scopus 로고    scopus 로고
    • Development and utilization of activated STAT3 detection assays for screening a library of secreted proteins
    • online ahead of print ADT-2010-0348-ver9-Fursov-1P
    • N. Fursov, I.V. Gates, T. Panavas, J. Giles-Komar, and G. Powers Development and utilization of activated STAT3 detection assays for screening a library of secreted proteins Assay Drug Dev. Tech. 3 2011 23 34 (online ahead of print ADT-2010-0348-ver9-Fursov-1P)
    • (2011) Assay Drug Dev. Tech. , vol.3 , pp. 23-34
    • Fursov, N.1    Gates, I.V.2    Panavas, T.3    Giles-Komar, J.4    Powers, G.5
  • 26
    • 79960121435 scopus 로고    scopus 로고
    • Automated quantitative assessment of proteins' biological function in protein knowledge bases
    • Epub
    • G. Mayr, G. Lepperdinger, and P. Lackner Automated quantitative assessment of proteins' biological function in protein knowledge bases Adv. Bioinform. 2008 897019 (Epub)
    • (2008) Adv. Bioinform. , pp. 897019
    • Mayr, G.1    Lepperdinger, G.2    Lackner, P.3
  • 27
    • 72149112754 scopus 로고    scopus 로고
    • STRA8 shuttles between nucleus and cytoplasm and displays transcriptional activity
    • M. Tedesco, G. La Sala, F. Barbagallo, M. De Felici, and D. Farini STRA8 shuttles between nucleus and cytoplasm and displays transcriptional activity J. Biol. Chem. 284 2009 35781 35793
    • (2009) J. Biol. Chem. , vol.284 , pp. 35781-35793
    • Tedesco, M.1    La Sala, G.2    Barbagallo, F.3    De Felici, M.4    Farini, D.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.