Simple Determination of Diacylglycerols Using Thin Layer Chromatography and Visible Spectrophotometry

Food Analytical Methods

Volumn 11, Issue 1, 2018, Pages 236-242

  Li, Qi ^a   Yu, Xiuzhu ^a   Yang, Yandie ^a   Liu, Xiaoli ^a  

^a NORTHWEST A F UNIVERSITY   (China)

Author keywords

Diacylglycerol; Quantitative determination; Thin layer chromatography; Visible spectrophotometry

Indexed keywords

CHROMATOGRAPHY; GLYCEROL; SPECTROPHOTOMETRY;

COLOR DEVELOPMENT; DETECTION LIMITS; DIACYLGLYCEROL; HIGHLY ACCURATE; MAXIMUM ABSORBANCE; QUANTITATIVE DETERMINATIONS; VIS SPECTROPHOTOMETRY; VISIBLE SPECTROPHOTOMETRIES;

THIN LAYER CHROMATOGRAPHY;

EID: 85023746404     PISSN: 19369751     EISSN: 1936976X     Source Type: Journal    
DOI: 10.1007/s12161-017-0993-0     Document Type: Article

References (40)

1. Ai M, Tanaka A, Shoji K, Ogita K, Hase T, Tokimitsu I, Shimokado K (2007) Suppressive effects of diacylglycerol oil on postprandial hyperlipidemia in insulin resistance and glucose intolerance. Atherosclerosis 195(2):398–403
2. Benard S, Arnhold J, Lehnert M, Schiller J, Arnold K (1999) Experiments towards quantification of saturated and polyunsaturated diacylglycerols by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. Chem Phys Lipids 100(1–2):115–125
3. Blasi F, Cossignani L, Simonetti MS, Damiani P (2007) Biocatalysed synthesis of sn-1,3-diacylglycerol oil from extra virgin olive oil. Enzyme Microb Tech 41(6–7):727–732
4. Blecker C, Paquot M, Lamberti I, Sensidoni A, Lognay G, Deroanne C (1997) Improved emulsifying and foaming of whey proteins after enzymic fat hydrolysis. Int J Food Sci 62(1):48–52
5. Blum J, Koehler WR (1970) Quantitative determination of glycerol, mono- and diglycerides by gas liquid chromatography. Lipids 5(7):601–606
6. Chattopadhyay S, Das S, Sen R (2011) Rapid and precise estimation of biodiesel by high performance thin layer chromatography. Appl Energ 88(12):5188–5192
7. Chen J, Dickinson E (1999) Effect of monoglycerides and diglycerol–esters on viscoelasticity of heat–set whey protein emulsion gels. Int J Food Sci Tech 34(5–6):493–501
8. Cheong LZ, Zhang H, Xu Y, Xu X (2009) Physical characterization of lard partial acylglycerols and their effects on melting and crystallization properties of blends with rapeseed oil. J Agr Food Chem 57:5020–5027
9. D'Alonzo RP, Kozarek WJ, Wade RL (1982) Glyceride composition of processed fats and oils as determined by glass capillary gas chromatography. J AmOil Chem Soc 59(7): 292–295
10. De Carvalho TC, Tosato F, Souza LM et al (2016) Thin layer chromatography coupled to paper spray ionization mass spectrometry for cocaine and its adulterants analysis. Forensic Sci Int 262:56–65
11. Eaton S, Shmueli E, Al-Mardini H, Bartlett K, Record CO (1995) An HPLC assay for sn-1,2-diacylglycerol. Clin Chim Acta 234(1–2):71–78
12. Fagan P, Wijesundera C, Watkins P (2004) Determination of mono- and di-acylglycerols in milk lipids. J Chromatogr A 1054(1–2):251–259
13. Feltes MMC, Villeneuve P, Baréa B, Barouh N, de Oliveira JV, de Oliveira D, Ninow JL (2012) Enzymatic production of monoacylglycerols (MAG) and diacylglycerols (DAG) from fish oil in a solvent-free system. J Am Oil Chem Soc 89(6):1057–1065
14. 31P NMR spectroscopy. J Agr Food Chem 50(8):2207–2213
15. Fuchs B, Suss R, Teuber K, Eibisch M, Schiller J (2011) Lipid analysis by thin-layer chromatography—a review of the current state. J Chromatogr A 1218(19):2754–2774
16. Fujii A, Allen TJ, Nestel PJ (2007) A 1,3-diacylglycerol-rich oil induces less atherosclerosis and lowers plasma cholesterol in diabetic apoE-deficient mice. Atherosclerosis 193(1):55–61
17. Fureby AM, Tian L, Adlercreutz P, Mattiasson B (1997) Preparation of diglycerides by lipase-catalyzed alcoholysis of triglycerides. Enzyme Microb Tech 20:198–206
18. García JM, Castellano JM, Pérez-Camino MC (1994) Quantitative determination of alpha and beta diglycerides in virgin oils extracted from stored fruits. Acta Hortic 356:351–353
19. Liu J, Lee T, BobikJr E, Guzman-Harty M, Hastilow C (1993) Quantitative determination of monoglycerides and diglycerides by high-performance liquid chromatography and evaporative light-scattering detection. J Am Oil Chem Soc 70(4):343–347
20. Lo SK, Tan CP, Long K, Yusoff MSA, Lai OM (2008) Diacylglycerol oil—properties, processes and products: a review. Food Bioprocess Tech 1(3):223–233
21. Long Z, Zhao M, Liu N, Liu D, Sun-Waterhouse D, Zhao Q (2015) Physicochemical properties of peanut oil-based diacylglycerol and their derived oil-in-water emulsions stabilized by sodium caseinate. Food Chem 184:105–113
22. Moh MH, Tang TS, Tan GH (2000) Optimization and validation of high-performance liquid chromatographic method for the determination of Dowtherm ATM in edible oils and oleochemicals. J Am Oil Chem Soc 77(10):1077–1083
23. Morita O, Soni MG (2009) Safety assessment of diacylglycerol oil as an edible oil: a review of the published literature. Food Chem Toxicol 47(1):9–21
24. Murase T, Mizuno T, Omachi T, Onizawa K, Komine Y, Kondo H, Hase T, Tokimitsu I (2001) Dietary diacylglycerol suppresses high fat and high sucrose diet-induced body fat accumulation in C57BL/6J mice. J Lipid Res 42(3):372–378
25. Okumura K, Hayashi K, Morishima I, Murase K, Matsui H, Toki Y, Ito T (1998) Simultaneous quantitation of ceramides and 1,2-diacylglycerol in tissues by Iatroscan thin-layer chromatography–flame-ionization detection. Lipids 33(5):529–532
26. Ota N, Soga S, Hase T, Tokimitsu I, Murase T (2007) Dietary diacylglycerol induces the regression of atherosclerosis in rabbits. J Nutr 137(5):1194–1199
27. Riisom T, Hoffmeyer L (1978) High performance liquid chromatography analyses of emulsifiers: I. Quantitative determinations of mono- and diacylglycerols of saturated fatty acids. J Am Oil Chem Soc 55(9):649–652
28. Rudkowska I, Roynette CE, Demonty I, Vanstone CA, Jew S, Jones PJ (2005) Diacylglycerol: efficacy and mechanism of action of an anti-obesity agent. Obesity 13(11):1864–1876
29. Saito S, Hernandez-Ono A, Ginsberg HN (2007) Dietary 1,3-diacylglycerol protects against diet-induced obesity and insulin resistance. Metabolism 56(11):1566–1575
30. Saito S, Yamaguchi T, Shoji K, Hibi M, Sugita T, Takase H (2010) Effect of low concentration of diacylglycerol on mildly postprandial hypertriglyceridemia. Atherosclerosis 213(2):539–544
31. Striby L, Lafont R, Goutx M (1999) Improvement in the Iatroscan thin-layer chromatographic–flame ionisation detection analysis of marine lipids. Separation and quantitation of monoacylglycerols and diacylglycerols in standards and natural samples. J Chromatogr A 849(2):371–380
32. Spyros A, Dais P (2000) Application of (31)P NMR spectroscopy in food analysis. 1. Quantitative determination of the mono- and diglyceride composition of olive oils. J Agr Food Chem 48(3):802–805
33. Tada N, Watanabe H, Matsuo N, Tokimitsu I, Okazaki M (2001) Dynamics of postprandial remnant-like lipoprotein particles in serum after loading of diacylglycerols. Clin Chim Acta 311(2):109–117
34. Taguchi H, Watanabe H, Onizawa K, Nagao T, Gotoh N, Yasukawa T, Tsushima R, Shimasaki H, Itakura H (2000) Double-blind controlled study on the effects of dietary diacylglycerol on postprandial serum and chylomicron triacylglycerol responses in healthy humans. J Am Coll Nutr 19:789–796
35. Tserng KY, Griffin R (2003) Quantitation and molecular species determination of diacylglycerols, phosphatidylcholines, ceramides, and sphingomyelins with gas chromatography. Anal Biochem 323(1):84–93
36. Wang W, Li T, Ning Z, Wang Y, Yang B, Yang X (2011) Production of extremely pure diacylglycerol from soybean oil by lipase-catalyzed glycerolysis. Enzyme Microb Tech 49(2):192–196
37. Warne TR, Robinson M (1991) A method for the simultaneous determination of alkylacylglycerol, diacylglycerol, monoalkylglycerol, monoacylglycerol, and cholesterol by high-performance liquid chromatography. Anal Biochem 198(2):302–307
38. Yamamoto K, Asakawa H, Tokunaga K, Watanabe H, Matsuo N, Tokimitsu I, Yagi N (2001) Long-term ingestion of dietary diacylglycerol lowers serum triacylglycerol in type II diabetic patients with hypertriglyceridemia. J Nutr 131(12):3204–3207
39. Yamamoto K, Tomonobu K, Asakawa H, Tokunaga K, Hase T, Tokimitsu I, Yagi N (2006) Diet therapy with diacylglycerol oil delays the progression of renal failure in type 2 diabetic patients with nephropathy. Diabetes Care 29(2):417–419
40. Yang B, Li QS, Yang JG, Li XF, Lin WT (2005) Analysis of diglyceride concentration by HPLC-RID. China Oils Fats 30(9):45–47