Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System

Stem Cell Reports

Volumn 8, Issue 6, 2017, Pages 1770-1783

  Rodrigues, Gonçalo M C ^a,b   Gaj, Thomas ^a   Adil, Maroof M ^c   Wahba, Joyce ^c   Rao, Antara T ^c   Lorbeer, Franziska K ^c   Kulkarni, Rishi U ^c   Diogo, Maria Margarida ^b   Cabral, Joaquim M S ^b   Miller, Evan W ^c   Hockemeyer, Dirk ^c   Schaffer, David V ^a,c  

^a University of California   (United States)

^b Instituto de Biotecnologia e Bioengenharia Lisboa   (Portugal)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

CRISPR Cas9 knockin; human pluripotent stem cells; NKX2.2 EGFP reporter cell line; oligodendrocyte precursor cells; scalable and defined oligodendrocyte differentiation; three dimensional culture systems

Indexed keywords

GUIDE RNA; MYELIN; OCTAMER TRANSCRIPTION FACTOR 4; OLIGODENDROCYTE TRANSCRIPTION FACTOR 2; RETINOIC ACID; SONIC HEDGEHOG PROTEIN; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR NKX2.2; BIOMATERIAL; HOMEODOMAIN PROTEIN; NKX-2.2 HOMEDOMAIN PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL LINEAGE; CELL MIGRATION; CELL SELECTION; CENTRAL NERVOUS SYSTEM; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; ELECTROPORATION; EXPRESSION VECTOR; FLOW CYTOMETRY; FLUORESCENCE; GENE EDITING; GENE TARGETING; HEK293T CELL LINE; HUMAN; HUMAN CELL; HUMAN EMBRYONIC STEM CELL; HYDROGEL; IMMUNOFLUORESCENCE; IN VIVO STUDY; MOUSE; NEURAL STEM CELL; NONHUMAN; OLIGODENDROCYTE PROGENITOR; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PURIFICATION; SCID MOUSE; SOUTHERN BLOTTING; WESTERN BLOTTING; ANIMAL; BRAIN; CELL CULTURE TECHNIQUE; CELL LINE; CHEMISTRY; CRISPR CAS SYSTEM; CYTOLOGY; GENETICS; METABOLISM; NONOBESE DIABETIC MOUSE; NUCLEAR REPROGRAMMING; OLIGODENDROCYTE PRECURSOR CELL; REPORTER GENE; TISSUE SCAFFOLD; TRANSPLANTATION; XENOGRAFT;

ANIMALS; BIOCOMPATIBLE MATERIALS; BRAIN; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELLULAR REPROGRAMMING; CRISPR-CAS SYSTEMS; GENES, REPORTER; HOMEODOMAIN PROTEINS; HUMANS; MICE; MICE, INBRED NOD; MICE, SCID; OLIGODENDROCYTE PRECURSOR CELLS; OLIGODENDROCYTE TRANSCRIPTION FACTOR 2; PLURIPOTENT STEM CELLS; TISSUE SCAFFOLDS; TRANSCRIPTION FACTORS; TRANSPLANTATION, HETEROLOGOUS;

EID: 85019626381     PISSN: 22136711     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.stemcr.2017.04.027     Document Type: Article

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