A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 52, 2013, Pages

  Lei, Yuguo ^a,b   Schaffer, David V ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; ALGINIC ACID; HYALURONIC ACID;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL FUNCTION; CELL GROWTH; CELL PROLIFERATION; CELL STRUCTURE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; DOPAMINERGIC NERVE CELL; ECTODERM; ENDODERM; HEART MUSCLE CELL; HUMAN; HUMAN CELL; HYDROGEL; KARYOTYPE; MESENCHYMAL STEM CELL; MESODERM; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; STEM CELL EXPANSION;

EID: 84891352376     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1309408110     Document Type: Article

References (52)

1. Thomson JA, et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391): 1145-1147.
2. Takahashi K, et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5): 861-872.
3. Chen G, et al. (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods 8(5): 424-429.
4. Schulz TC, et al. (2012) A scalable system for production of functional pancreatic progenitors from human embryonic stem cells. PLoS One 7(5):e37004.
5. Lindvall O, Kokaia Z, Martinez-Serrano A (2004) Stem cell therapy for human neurodegenerative disorders-how to make it work. Nat Med 10(Suppl):S42-S50.
6. Badylak SF, Taylor D, Uygun K (2011) Whole-organ tissue engineering: Decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng 13: 27-53.
7. McNeish J (2004) Embryonic stem cells in drug discovery. Nat Rev Drug Discov 3(1): 70-80.
8. Desbordes SC, Studer L (2013) Adapting human pluripotent stem cells to highthroughput and high-content screening. Nat Protoc 8(1): 111-130.
9. Serra M, Brito C, Correia C, Alves PM (2012) Process engineering of human pluripotent stem cells for clinical application. Trends Biotechnol 30(6): 350-359.
10. Kriks S, et al. (2011) Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease. Nature 480(7378): 547-551.
11. Laflamme MA, Murry CE (2005) Regenerating the heart. Nat Biotechnol 23(7): 845-856.
12. Roger VL, et al. (2012) Heart disease and stroke statistics - 2012 update: A report from the American Heart Association. Circulation 125(1):e2-e220.
13. Zang R, Li D, Tang I, Wang J, Yang S (2012) Cell-based assays in high-throughput screening for drug discovery. Int J Biotechnol Wellness Ind 1: 31-51.
14. Ohgushi M, Sasai Y (2011) Lonely death dance of human pluripotent stem cells: ROCKing between metastable cell states. Trends Cell Biol 21(5): 274-282.
15. Peerani R, et al. (2007) Niche-mediated control of human embryonic stem cell selfrenewal and differentiation. EMBO J 26(22): 4744-4755.
16. Villa-Diaz LG, Ross AM, Lahann J, Krebsbach PH (2013) Concise review: The evolution of human pluripotent stem cell culture: From feeder cells to synthetic coatings. Stem Cells 31(1): 1-7.
17. McDevitt TC, Palecek SP (2008) Innovation in the culture and derivation of pluripotent human stem cells. Curr Opin Biotechnol 19(5): 527-533.
18. Chen VC, et al. (2012) Scalable GMP compliant suspension culture system for human ES cells. Stem Cell Res (Amst) 8(3): 388-402.
19. Steiner D, et al. (2010) Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol 28(4): 361-364.
20. Amit M, et al. (2011) Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells. Nat Protoc 6(5): 572-579.
21. Zweigerdt R, Olmer R, Singh H, Haverich A, Martin U (2011) Scalable expansion of human pluripotent stem cells in suspension culture. Nat Protoc 6(5): 689-700.
22. Nie Y, Bergendahl V, Hei DJ, Jones JMPS, Palecek SP (2009) Scalable culture and cryopreservation of human embryonic stem cells on microcarriers. Biotechnol Prog 25(1): 20-31.
23. Chen AK, Chen X, Choo AB, Reuveny S, Oh SK (2011) Critical microcarrier properties affecting the expansion of undifferentiated human embryonic stem cells. Stem Cell Res (Amst) 7(2): 97-111.
24. Serra M, et al. (2011) Microencapsulation technology: A powerful tool for integrating expansion and cryopreservation of human embryonic stem cells. PLoS One 6(8): e23212.
25. Unger C, Skottman H, Blomberg P, Dilber MS, Hovatta O (2008) Good manufacturing practice and clinical-grade human embryonic stem cell lines. Hum Mol Genet 17(R1): R48-R53.
26. Medina RJ, Kataoka K, Takaishi M, Miyazaki M, Huh NH (2006) Isolation of epithelial stem cells from dermis by a three-dimensional culture system. J Cell Biochem 98(1): 174-184.
27. Chayosumrit M, Tuch B, Sidhu K (2010) Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Biomaterials 31(3): 505-514.
28. Stenberg J, et al. (2011) Sustained embryoid body formation and culture in a nonlaborious three dimensional culture system for human embryonic stem cells. Cytotechnology 63(3): 227-237.
29. Gerecht S, et al. (2007) Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells. Proc Natl Acad Sci USA 104(27): 11298-11303.
30. Watanabe K, et al. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25(6): 681-686.
31. Xu Y, et al. (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107(18): 8129-8134.
32. Ohgushi M, et al. (2010) Molecular pathway and cell state responsible for dissociationinduced apoptosis in human pluripotent stem cells. Cell Stem Cell 7(2): 225-239.
33. Park I-H, et al. (2008) Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451(7175): 141-146.
34. Lian X, et al. (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci USA 109(27):E1848-E1857.
35. Lindvall O (2013) Developing dopaminergic cell therapy for Parkinson's disease - give up or move forward? Mov Disord 28(3): 268-273.
36. Mendez I, et al. (2008) Dopamine neurons implanted into people with Parkinson's disease survive without pathology for 14 years. Nat Med 14(5): 507-509.
37. Kirkeby A, et al. (2012) Generation of regionally specified neural progenitors and functional neurons from human embryonic stem cells under defined conditions. Cell Rep 1(6): 703-714.
38. Xu R-H, et al. (2008) NANOG is a direct target of TGFbeta/activin- mediated SMAD signaling in human ESCs. Cell Stem Cell 3(2): 196-206.
39. Vallier L, Alexander M, Pedersen RA (2005) Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118(Pt 19): 4495-4509.
40. James D, Levine AJ, Besser D, Hemmati-Brivanlou A (2005) TGFbeta/activin/nodal signaling is necessary for the maintenance of pluripotency in human embryonic stem cells. Development 132(6): 1273-1282.
41. Hsieh MH, Nguyen HT (2005) Molecular mechanism of apoptosis induced by mechanical forces. Int Rev Cytol 245: 45-90.
42. Lee G, Chambers SM, Tomishima MJ, Studer L (2010) Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc 5(4): 688-701.
43. Hu B-Y, Zhang S-C (2009) Differentiation of spinal motor neurons from pluripotent human stem cells. Nat Protoc 4(9): 1295-1304.
44. Keirstead HS, et al. (2005) Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury. J Neurosci 25(19): 4694-4705.
45. Sharp J, Frame J, Siegenthaler M, Nistor G, Keirstead HS (2010) Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants improve recovery after cervical spinal cord injury. Stem Cells 28(1): 152-163.
46. Kroon E, et al. (2008) Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol 26(4): 443-452.
47. Duan Y, et al. (2010) Differentiation and characterization of metabolically functioning hepatocytes from human embryonic stem cells. Stem Cells 28(4): 674-686.
48. Osakada F, Ikeda H, Sasai Y, Takahashi M (2009) Stepwise differentiation of pluripotent stem cells into retinal cells. Nat Protoc 4(6): 811-824.
49. Cheung C, Sinha S (2011) Human embryonic stem cell-derived vascular smooth muscle cells in therapeutic neovascularisation. J Mol Cell Cardiol 51(5): 651-664.
50. Descamps B, Emanueli C (2012) Vascular differentiation from embryonic stem cells: Novel technologies and therapeutic promises. Vascul Pharmacol 56(5-6): 267-279.
51. Trounson A, Thakar RG, Lomax G, Gibbons D (2011) Clinical trials for stem cell therapies. BMC Med 9:52.
52. Chambers SM, et al. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27(3): 275-280.