Separation technologies for stem cell bioprocessing

Biotechnology and Bioengineering

Volumn 109, Issue 11, 2012, Pages 2699-2709

  Diogo, Maria Margarida ^a   da Silva, Cláudia Lobato ^a   Cabral, Joaquim M S ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Bioprocessing; Integration; Isolation; Purification; Regenerative medicine; Stem cells

Indexed keywords

ADULT STEM CELLS; APTAMERS; BIOLOGICAL FEATURES; BIOPROCESSING; CELL LABELING; CELL SEPARATION; CLINICAL UTILITY; CRITICAL ASSESSMENT; DEVELOPMENTAL BIOLOGY; DRUG DISCOVERY; FUNDAMENTAL PRINCIPLES; HEMATOPOIETIC STEM/PROGENITOR CELLS; HETEROGENEOUS POPULATIONS; HIGH-RESOLUTION METHODS; HIGHER RESOLUTION; HUMAN DISEASE; ISOLATION; ISOLATION AND PURIFICATION; MICROSCALE TECHNOLOGY; PLURIPOTENT STEM CELLS; POTENTIAL APPLICATIONS; REGENERATIVE MEDICINE; SEPARATION METHODS; SEPARATION PROCESS; SEPARATION TECHNOLOGIES; TUMORIGENIC CELLS;

CYTOLOGY; INTEGRATION; MEDICINE; PURIFICATION; SEPARATION;

STEM CELLS;

APTAMER; CD34 ANTIGEN;

AFFINITY CHROMATOGRAPHY; ANTIBODY AFFINITY; ANTIGEN EXPRESSION; BIOCHEMISTRY; BIOPROCESS; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CELL MATURATION; CELL RENEWAL; CELL SELECTION; CELL SEPARATION; CENTRIFUGATION; ELECTROPHORESIS; FIELD FLOW FRACTIONATION; FILTRATION; FLUORESCENCE ACTIVATED CELL SORTING; HEMATOPOIETIC STEM CELL; HUMAN; IMMUNOADSORPTION; MESENCHYMAL STEM CELL; MICROFLUIDICS; NEURAL STEM CELL; NONHUMAN; PHENOTYPE; PHYSICAL CHEMISTRY; PURIFICATION; REVIEW; SEPARATION TECHNIQUE; STEM CELL; STEM CELL TRANSPLANTATION;

CELL SEPARATION; HUMANS; REGENERATIVE MEDICINE; STEM CELLS;

EID: 84866748912     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24706     Document Type: Review

References (73)

1. Andrade PZ, da Silva CL, dos Santos F, Almeida-Porada G, Cabral JMS. 2011. Initial CD34+ cell-enrichment of cord blood determines hematopoietic stem/progenitor cell yield upon ex vivo expansion. J Cell Biochem 112(7):1822-1831.
2. Aubert J, Stavridis MP, Tweedie S, O'Reilly M, Vierlinger K, Li M, Ghazal P, Pratt T, Mason JO, Roy D, Smith A. 2003. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci USA 100(Suppl 1):11836-11841.
3. Barry F, Boynton R, Murphy M, Haynesworth S, Zaia J. 2001. The SH-3 and SH-4 antibodies recognize distinct epitopes on CD73 from human mesenchymal stem cells. Biochem Biophys Res Commun. 289(2):519-524.
4. Bonanno G, Perillo A, Rutella S, De Ritis DG, Mariotti A, Marone M, Meoni F, Scambia G, Leone G, Mancuso S, Pierelli L. 2004. Clinical isolation and functional characterization of cord blood CD133+ hematopoietic progenitor cells. Transfusion 44(7):1087-1097.
5. Brown M, Wittwer C. 2000. Flow cytometry: Principles and clinical applications in hematology. Clin Chem 46(8 Pt 2):1221-1229.
6. Cabral JMS. 2007. Cell partitioning in aqueous two-phase polymer systems. Adv Biochem Eng Biotechnol 106:151-171.
7. Capela A, Temple S. 2002. LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal. Neuron 35(5):865-875.
8. Caplan AI. 2007. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol 213(2):341-347.
9. Caplan AI. 2009. Why are MSCs therapeutic? New data: New insight. J Pathol 217(2):318-324.
10. Cardoso AA, Watt SM, Batard P, Li ML, Hatzfeld A, Genevier H, Hatzfeld J. 1995. An improved panning technique for the selection of CD34+ human bone marrow hematopoietic cells with high recovery of early progenitors. Exp Hematol 23(5):407-412.
11. Chou S, Chu P, Hwang W, Lodish H. 2010. Expansion of human cord blood hematopoietic stem cells for transplantation. Cell Stem Cell 7(4):427-428.
12. Comte I, Battu S, Mathonnet M, Bessette B, Lalloue F, Cardot P, Ayer-Le Lievre C. 2006. Neural stem cell separation from the embryonic avian olfactory epithelium by sedimentation field-flow fractionation. J Chromatogr B Analyt Technol Biomed Life Sci 843(2):175-182.
13. Corti S, Locatelli F, Papadimitriou D, Donadoni C, Del Bo R, Fortunato F, Strazzer S, Salani S, Bresolin N, Comi GP. 2005. Multipotentiality, homing properties, and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells. FASEB J 19(13):1860-1862.
14. da Silva CL, Goncalves R, Crapnell KB, Cabral JMS, Zanjani ED, Almeida-Porada G. 2005. A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells. Exp Hematol 33(7):828-835.
15. da Silva CL, Goncalves R, Porada CD, Ascensao JL, Zanjani ED, Cabral JMS, Almeida-Porada G. 2009. Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics. J Cell Physiol 220(1):102-111.
16. Didar TF, Tabrizian M. 2010. Adhesion based detection, sorting and enrichment of cells in microfluidic Lab-on-Chip devices. Lab Chip 10(22):3043-3053.
17. Flanagan LA, Lu J, Wang L, Marchenko SA, Jeon NL, Lee AP, Monuki ES. 2008. Unique dielectric properties distinguish stem cells and their differentiated progeny. Stem Cells 26(3):656-665.
18. Fong CY, Peh GS, Gauthaman K, Bongso A. 2009. Separation of SSEA-4 and TRA-1-60 labelled undifferentiated human embryonic stem cells from a heterogeneous cell population using magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). Stem Cell Rev 5(1):72-80.
19. Fukuda H, Takahashi J, Watanabe K, Hayashi H, Morizane A, Koyanagi M, Sasai Y, Hashimoto N. 2006. Fluorescence-activated cell sorting-based purification of embryonic stem cell-derived neural precursors averts tumor formation after transplantation. Stem Cells 24(3):763-771.
20. Geens M, Van de Velde H, De Block G, Goossens E, Van Steirteghem A, Tournaye H. 2007. The efficiency of magnetic-activated cell sorting and fluorescence-activated cell sorting in the decontamination of testicular cell suspensions in cancer patients. Hum Reprod 22(3):733-742.
21. Goncalves R, Lobato da Silva C, Cabral JM, Zanjani ED, Almeida-Porada G. 2006. A Stro-1(+) human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system. Exp Hematol 34(10):1353-1359.
22. Gonzalez-Gonzalez M, Vazquez-Villegas P, Garcia-Salinas C, Rito-Palomares M. 2012. Current strategies and challenges for the purification of stem cells. J Chem Technol Biotechnol 87(1):2-10.
23. Greenberg AW, Kerr WG, Hammer DA. 2000. Relationship between selectin-mediated rolling of hematopoietic stem and progenitor cells and progression in hematopoietic development. Blood 95(2):478-486.
24. Gronthos S, Zannettino AC. 2008. A method to isolate and purify human bone marrow stromal stem cells. Methods Mol Biol. 449:45-57.
25. Grutzkau A, Radbruch A. 2010. Small but mighty: How the MACS-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20 years. Cytometry A 77(7):643-647.
26. Guglielmi L, Battu S, Le Bert M, Faucher JL, Cardot PJ, Denizot Y. 2004. Mouse embryonic stem cell sorting for the generation of transgenic mice by sedimentation field-flow fractionation. Anal Chem 76(6):1580-1585.
27. Guo KT, SchAfer R, Paul A, Gerber A, Ziemer G, Wendel HP. 2006. A new technique for the isolation and surface immobilization of mesenchymal stem cells from whole bone marrow using high-specific DNA aptamers. Stem Cells 24(10):2220-2231.
28. Hammer DA, Apte SM. 1992. Simulation of cell rolling and adhesion on surfaces in shear flow: General results and analysis of selectin-mediated neutrophil adhesion. Biophys J 63(1):35-57.
29. Hardt S, Hahn T. 2012. Microfluidics with aqueous two-phase systems. Lab Chip 12(3):434-442.
30. Higuchi A, Iizuka A, Gomei Y, Miyazaki T, Sakurai M, Matsuoka Y, Natori SH. 2006. Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes. J Biomed Mater Res A 78(3):491-499.
31. Higuchi A, Sekiya M, Gomei Y, Sakurai M, Chen WY, Egashira S, Matsuoka Y. 2008. Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes. J Biomed Mater Res A 85(4):853-861.
32. Ito K, Aoyama T, Fukiage K, Otsuka S, Furu M, Jin Y, Nasu A, Ueda M, Kasai Y, Ashihara E, Kimura S, Maekawa T, Kobayashi A, Yoshida S, Niwa H, Otsuka T, Nakamura T, Toguchida J. 2010. A novel method to isolate mesenchymal stem cells from bone marrow in a closed system using a device made by nonwoven fabric. Tissue Eng Part C Methods 16(1):81-91.
33. Johnsen HE, Hutchings M, Taaning E, Rasmussen T, Knudsen LM, Hansen SW, Andersen H, Gaarsdal E, Jensen L, Nikolajsen K, Kjaesgârd E, Hansen NE. 1999. Selective loss of progenitor subsets following clinical CD34+ cell enrichment by magnetic field, magnetic beads or chromatography separation. Bone Marrow Transplant 24(12):1329-1336.
34. Jones EA, Kinsey SE, English A, Jones RA, Straszynski L, Meredith DM, Markham AF, Jack A, Emery P, McGonagle D. 2002. Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum. 46(12):3349-3360.
35. Kim SM, Lee SH, Suh KY. 2008. Cell research with physically modified microfluidic channels: A review. Lab Chip 8(7):1015-1023.
36. Kumar A, Bhardwaj A. 2008. Methods in cell separation for biomedical application: Cryogels as a new tool. Biomed Mater 3(3):034008.
37. Kumar A, Srivastava A. 2010. Cell separation using cryogel-based affinity chromatography. Nat Protoc 5(11):1737-1747.
38. Kumar A, Kamihira M, Galaev IY, Mattiasson B, Iijima S. 2001. Type-specific separation of animal cells in aqueous two-phase systems using antibody conjugates with temperature-sensitive polymers. Biotechnol Bioeng 75(5):570-580.
39. Labeed FH, Lu J, Mulhall HJ, Marchenko SA, Hoettges KF, Estrada LC, Lee AP, Hughes MP, Flanagan LA. 2011. Biophysical characteristics reveal neural stem cell differentiation potential. PLoS ONE 6(9):e25458.
40. Laflamme MA, Chen KY, Naumova AV, Muskheli V, Fugate JA, Dupras SK, Reinecke H, Xu C, Hassanipour M, Police S, O'Sullivan C, Collins L, Chen Y, Minami E, Gill EA, Ueno S, Yuan C, Gold J, Murry CE. 2007. Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol 25(9):1015-1024.
41. Lennon DP, Caplan AI. 2006. Isolation of human marrow-derived mesenchymal stem cells. Exp Hematol 34(11):1604-1605.
42. Levenberg S, Ferreira LS, Chen-Konak L, Kraehenbuehl TP, Langer R. 2010. Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells. Nat Protoc 5(6):1115-1126.
43. Loureiro J, Andrade PZ, Cardoso S, da Silva CL, Cabral JMS, Freitas PP. 2011. Magnetoresistive chip cytometer. Lab Chip 11(13):2255-2261.
44. Lozinsky VI, Galaev IY, Plieva FM, Savina IN, Jungvid H, Mattiasson B. 2003. Polymeric cryogels as promising materials of biotechnological interest. Trends Biotechnol 21(10):445-451.
45. Mahara A, Yamaoka T. 2010a. Antibody-immobilized column for quick cell separation based on cell rolling. Biotechnol Prog 26(2):441-447.
46. Mahara A, Yamaoka T. 2010b. Continuous separation of cells of high osteoblastic differentiation potential from mesenchymal stem cells on an antibody-immobilized column. Biomaterials 31(14):4231-4237.
47. Majore I, Moretti P, Hass R, Kasper C. 2009. Identification of subpopulations in mesenchymal stem cell-like cultures from human umbilical cord. Cell Commun Signal 7:6.
48. Muller M, Fleischmann BK, Selbert S, Ji GJ, Endl E, Middeler G, Muller OJ, Schlenke P, Frese S, Wobus AM, Hescheler J, Katus HA, Franz WM. 2000. Selection of ventricular-like cardiomyocytes from ES cells in vitro. FASEB J 14(15):2540-2548.
49. Negrin RS, Atkinson K, Leemhuis T, Hanania E, Juttner C, Tierney K, Hu WW, Johnston LJ, Shizurn JA, Stockerl-Goldstein KE, Blume KG, Weissman IL, Bower S, Baynes R, Dansey R, Karanes C, Peters W, Klein J. 2000. Transplantation of highly purified CD34+Thy-1+ hematopoietic stem cells in patients with metastatic breast cancer. Biol Blood Marrow Transplant 6(3):262-271.
50. Nery AA, Wrenger C, Ulrich H. 2009. Recognition of biomarkers and cell-specific molecular signatures: Aptamers as capture agents. J Sep Sci 32(10):1523-1530.
51. Parekkadan B, Sethu P, van Poll D, Yarmush ML, Toner M. 2007. Osmotic selection of human mesenchymal stem/progenitor cells from umbilical cord blood. Tissue Eng 13(10):2465-2473.
52. Pethig R, Menachery A, Pells S, De Sousa P. 2010. Dielectrophoresis: A review of applications for stem cell research. J Biomed Biotechnol 2010:182581.
53. Pruszak J, Sonntag KC, Aung MH, Sanchez-Pernaute R, Isacson O. 2007. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells 25(9):2257-2268.
54. Reschiglian P, Zattoni A, Roda B, Michelini E, Roda A. 2005. Field-flow fractionation and biotechnology. Trends Biotechnol 23(9):475-483.
55. Rietze RL, Valcanis H, Brooker GF, Thomas T, Voss AK, Bartlett PF. 2001. Purification of a pluripotent neural stem cell from the adult mouse brain. Nature 412(6848):736-739.
56. Roda B, Lanzoni G, Alviano F, Zattoni A, Costa R, Di Carlo A, Marchionni C, Franchina M, Ricci F, Tazzari PL, Pagliaro P, Scalinci SZ, Bonsi L, Reschiglian P, Bagnara GP. 2009a. A novel stem cell tag-less sorting method. Stem Cell Rev 5(4):420-427.
57. Roda B, Reschiglian P, Alviano F, Lanzoni G, Bagnara GP, Ricci F, Buzzi M, Tazzari PL, Pagliaro P, Michelini E, Roda A. 2009b. Gravitational field-flow fractionation of human hemopoietic stem cells. J Chromatogr A 1216(52):9081-9087.
58. Santos F, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, Campbell AM, Boucher S, Vemuri MC, Silva CL, Cabral JMS. 2011. Toward a clinical-grade expansion of mesenchymal stem cells from human sources: A microcarrier-based culture system under xeno-free conditions. Tissue Eng Part C Methods 17(12):1201-1210.
59. Schriebl K, Lim S, Choo A, Tscheliessnig A, Jungbauer A. 2010. Stem cell separation: A bottleneck in stem cell therapy. Biotechnol J 5(1):50-61.
60. Shibata H, Ageyama N, Tanaka Y, Kishi Y, Sasaki K, Nakamura S, Muramatsu S, Hayashi S, Kitano Y, Terao K, Hanazono Y. 2006. Improved safety of hematopoietic transplantation with monkey embryonic stem cells in the allogeneic setting. Stem Cells 24(6):1450-1457.
61. Sousa AF, Andrade PZ, Pirzgalska RM, Galhoz TM, Azevedo AM, da Silva CL, Aires-Barros MR, Cabral JMS. 2011. A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning. Biotechnol Lett 33(12):2373-2377.
62. Stephens M, Talary MS, Pethig R, Burnett AK, Mills KI. 1996. The dielectrophoresis enrichment of CD34+ cells from peripheral blood stem cell harvests. Bone Marrow Transplant 18(4):777-782.
63. Sundberg M, Andersson PH, Åkesson E, Odeberg J, Holmberg L, Inzunza J, Falci S, Öhman J, Suuronen R, Skottman H, Lehtimäki K, Hovatta O, Narkilahti S, Sundström E. 2011. Markers of pluripotency and differentiation in human neural precursor cells derived from embryonic stem cells and CNS tissue. Cell Transplant 20(2):177-191.
64. Talary MS, Mills KI, Hoy T, Burnett AK, Pethig R. 1995. Dielectrophoretic separation and enrichment of CD34+ cell subpopulation from bone marrow and peripheral blood stem cells. Med Biol Eng Comput 33(2):235-237.
65. Tang C, Lee AS, Volkmer JP, Sahoo D, Nag D, Mosley AR, Inlay MA, Ardehali R, Chavez SL, Pera RR, Behr B, Wu JC, Weissman IL, Drukker M. 2011. An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Nat Biotechnol 29(9):829-834.
66. Tondreau T, Lagneaux L, Dejeneffe M, Delforge A, Massy M, Mortier C, Bron D. 2004. Isolation of BM mesenchymal stem cells by plastic adhesion or negative selection: phenotype, proliferation kinetics and differentiation potential. Cytotherapy 6(4):372-379.
67. Uchida N, Buck DW, He D, Reitsma MJ, Masek M, Phan TV, Tsukamoto AS, Gage FH, Weissman IL. 2000. Direct isolation of human central nervous system stem cells. Proc Natl Acad Sci USA 97(26):14720-14725.
68. Uosaki H, Fukushima H, Takeuchi A, Matsuoka S, Nakatsuji N, Yamanaka S, Yamashita JK. 2011. Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression. PLoS ONE 6(8):e23657.
69. Wang P, Rodriguez RT, Wang J, Ghodasara A, Kim SK. 2011a. Targeting SOX17 in human embryonic stem cells creates unique strategies for isolating and analyzing developing endoderm. Cell Stem Cell 8(3):335-346.
70. Wang X, Chen S, Kong M, Wang Z, Costa KD, Li RA, Sun D. 2011b. Enhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies. Lab Chip 11(21):3656-3662.
71. Wang YC, Nakagawa M, Garitaonandia I, Slavin I, Altun G, Lacharite RM, Nazor KL, Tran HT, Lynch CL, Leonardo TR, Liu Y, Peterson SE, Laurent LC, Yamanaka S, Loring JF. 2011c. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Res 21(11):1551-1563.
72. Weissman IL, Shizuru JA. 2008. The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases. Blood 112(9):3543-3553.
73. Yuan SH, Martin J, Elia J, Flippin J, Paramban RI, Hefferan MP, Vidal JG, Mu Y, Killian RL, Israel MA, Emre N, Marsala S, Marsala M, Gage FH, Goldstein LS, Carson CT. 2011. Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells. PLoS ONE 6(3):e17540.