Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis

BMC Genomics

Volumn 16, Issue 1, 2015, Pages

  Pan, Luyuan ^a,e   Shah, Arish N ^a   Phelps, Ian G ^b   Doherty, Dan ^b   Johnson, Eric A ^c   Moens, Cecilia B ^a,d  

^a USA   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF OREGON   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

^e INSTITUTE OF HYDROBIOLOGY   (China)

Author keywords

Next generation sequencing; PELE analysis; Rare mutation detection; TILLING; Zebrafish

Indexed keywords

GENOMIC DNA; DNA; ETHYLNITROSOUREA; RNA SPLICING; STOP CODON;

ADULT; ALLELE; AMPLICON; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; EXON; FISH GENETICS; GENE IDENTIFICATION; GENE TARGETING; GENETIC CODE; GENETIC PROCEDURES; GENOME SIZE; HETEROZYGOTE; INDUCED MUTATION; MALE; NEXT GENERATION SEQUENCING; NONHUMAN; NONSENSE MUTATION; PROGENY; TARGETING INDUCED LOCAL LESIONS IN GENOME; ZEBRA FISH; ANIMAL; DNA SEQUENCE; DRUG EFFECTS; GENE LIBRARY; GENETICS; GENOME; GENOMICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; METABOLISM; MUTATION; PROCEDURES; RNA SPLICING; SPERMATOZOON; STANDARDS; STOP CODON;

DANIO RERIO;

ANIMALS; CODON, NONSENSE; DNA; ETHYLNITROSOUREA; GENE LIBRARY; GENOME; GENOMICS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MALE; MUTATION; RNA SPLICE SITES; SEQUENCE ANALYSIS, DNA; SPERMATOZOA; ZEBRAFISH;

EID: 85019233363     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/s12864-015-1263-4     Document Type: Article

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