Progress in TILLING as a tool for functional genomics and improvement of crops

Journal of Integrative Plant Biology

Volumn 56, Issue 5, 2014, Pages 425-443

  Chen, Liang ^a   Hao, Liugen ^a   Parry, Martin A J ^b   Phillips, Andrew L ^b   Hu, Yin Gang ^a  

^a NORTHWEST A F UNIVERSITY   (China)

^b ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Crop breeding; Functional genomics; Mutation detection; TILLING

Indexed keywords

ALLELE; CROP IMPROVEMENT; GENETICALLY MODIFIED ORGANISM; GENOME; GENOMICS; MUTATION; PHENOTYPE; PLANT BREEDING;

CROP; GENETICS; GENOMICS; MUTAGENESIS; PHYSIOLOGY; POINT MUTATION; PROCEDURES;

CROPS, AGRICULTURAL; GENOMICS; MUTAGENESIS; POINT MUTATION;

EID: 84900004717     PISSN: 16729072     EISSN: 17447909     Source Type: Journal    
DOI: 10.1111/jipb.12192     Document Type: Article

References (118)

1. Allen AM, Barker GL, Berry ST, Coghill JA, Gwilliam R, Kirby S, Robinson P, Brenchley RC, D'Amore R, McKenzie N, Waite D, Hall A, Bevan M, Hall N, Edwards KJ (2011) Transcript-specific, single-nucleotide polymorphism discovery and linkage analysis in hexaploid bread wheat (Triticum aestivum L.). Plant Biotechnol J 9: 1086-1099
2. Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657
3. Blomstedt CK, Gleadow RM, O'Donnell N, Naur P, Jensen K, Laursen T, Olsen CE, Stuart P, Hamill JD, Moller BL, Neale AD (2012) A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L. Moench resulting in acyanogenic forage production. Plant Biotechnol J 10: 54-66
4. Bommert P, Nagasawa NS, Jackson D (2013) Quantitative variation in maize kernel row number is controlled by the FASCIATED EAR2 locus. Nat Genet 45: 334-337
5. Botticella E, Sestili F, Hernandez-Lopez A, Phillips A, Lafiandra D (2011) High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes. BMC Plant Biol 11: 156
6. Brenchley R, Spannagl M, Pfeifer M, Barker GL, D'Amore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Huo N, Luo MC, Sehgal S, Gill B, Kianian S, Anderson O, Kersey P, Dvorak J, McCombie WR, Hall A, Mayer KF, Edwards KJ, Bevan MW, Hall N (2012) Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature 491: 705-710
7. Caldwell DG, McCallum N, Shaw P, Muehlbauer GJ, Marshall DF, Waugh R (2004) A structured mutant population for forward and reverse genetics in Barley (Hordeum vulgare L.). Plant J 40: 143-150
8. Chawade A, Sikora P, Brautigam M, Larsson M, Vivekanand V, Nakash MA, Chen T, Olsson O (2010) Development and characterization of an oat TILLING-population and identification of mutations in lignin and beta-glucan biosynthesis genes. BMC Plant Biol 10: 86
9. Chen L, Huang LZ, Min DH, Phillips A, Wang SQ, Madgwick PJ, Parry MAJ, Hu YG (2012) Development and characterization of a new TILLING population of common bread wheat (Triticum aestivum L.). PLoS ONE 7: e41570
10. Chen L, Wang SQ, Hu YG (2011) Detection of SNPs in the VRN-A1 gene of common wheat (Triticum aestivum L.) by a modified EcoTILLING method using agarose gel electrophoresis. Aust J Crop Sci 5: 321-329
11. Colbert T, Till BJ, Tompa R, Reynolds S, Steine MN, Yeung AT, McCallum CM, Comai L, Henikoff S (2001) High-throughput screening for induced point mutations. Plant Physiol 126: 480-484
12. Comai L, Young K, Till BJ, Reynolds SH, Greene EA, Codomo CA (2004) Efficient discovery of DNA polymorphisms in natural populations by EcoTILLING. Plant J 37: 778-786
13. Cooper JL, Till BJ, Laport RG, Darlow MC, Kleffner JM, Jamai A, El-Mellouki T, Liu S, Ritchie R, Nielsen N, Bilyeu KD, Meksem K, Comai L, Henikoff S (2008) TILLING to detect induced mutations in soybean. BMC Plant Biol 8: 9
14. Dahmani-Mardas F, Troadec C, Boualem A, Leveque S, Abdullah A (2010) Engineering melon plants with improved fruit shelf life using the TILLING approach. PLoS ONE 5: e15776
15. Dalmais M, Antelme S, Ho-Yue-Kuang S, Wang Y, Darracq O (2013) A TILLING platform for functional genomics in Brachypodium distachyon. PLoS ONE 8: e65503
16. Dierking EC, Bilyeu KD (2009) New sources of soybean seed meal and oil composition traits identified through TILLING. BMC Plant Biol 9: 89
17. Dong C, Dalton-Morgan J, Vincent K, Sharp P (2009a) A modified TILLING method for wheat breeding. Plant Gen 2: 39
18. Dong C, Vincent K, Sharp P (2009b) Simultaneous mutation detection of three homoeologous genes in wheat by high resolution melting analysis and mutation surveyor. BMC Plant Biol 9: 143
19. Elias R, Till BJ, Mba C, Al-Safadi B (2009) Optimizing TILLING and EcoTILLING techniques for potato (Solanum tuberosum L.). BMC Res Notes 2: 141
20. FAO (2009) How to Feed the World in 2050. Food and Agriculture Organization of the United Nations Food and Agriculture Organization of the United Nations, Rome, Italy
21. Feiz L, Martin JM, Giroux MJ (2009) Creation and functional analysis of new puroindoline alleles in Triticum aestivum. Theor Appl Genet 118: 247-257
22. Fitzgerald TL, Kazan K, Li Z, Morell MK, Manners JM (2010) A high-throughput method for the detection of homologous gene deletions in hexaploid wheat. BMC Plant Biol 10: 264
23. Frerichmann SL, Kirchhoff M, Müller AE, Scheidig AJ, Jung C, Kopisch-Obuch FJ (2013) EcoTILLING in beta vulgaris reveals polymorphisms in the FLC-like gene BvFL1 that are associated with annuality and winter hardiness. BMC Plant Biol 13: 52
24. Fu D, Uauy C, Blechl A, Dubcovsky J (2007) RNA interference for wheat functional gene analysis. Transgenic Res 16: 689-701
25. Gady AL, Hermans FW, Van de Wal MH, van Loo EN, Visser RG, Bachem CW (2009) Implementation of two high through-put techniques in a novel application: Detecting point mutations in large EMS mutated plant populations. Plant Methods 5: 13
26. Galeano CH, Gomez M, Rodriguez LM, Blair MW (2009) CEL I nuclease digestion for SNP discovery and marker development in common bean (Phaseolus vulgaris L.). Crop Sci 49: 381
27. Gaul H (1958) Present aspects of induced mutations in plant breeding. Euphytica 7: 275-289
28. Gepts P, Papa R (2002) Evolution during domestication. Encycl Life Sci: 1-7
29. Gilchrist E, Haughn G (2010) Reverse genetics techniques: Engineering loss and gain of gene function in plants. Brief Funct Genomics 9: 103-110
30. Gonzalez M, Xu M, Esteras C, Roig C, Monforte AJ, Troadec C, Pujol M, Nuez F, Bendahmane A, Garcia-Mas J, Pico B (2011) Towards a TILLING platform for functional genomics in Piel de Sapo melons. BMC Res Notes 4: 289
31. Gottwald S, Bauer P, Komatsuda T, Lundqvist U, Stein N (2009) TILLING in the two-rowed barley cultivar 'Barke' reveals preferred sites of functional diversity in the gene HvHox1. BMC Res Notes 2: 258
32. Graybosch RA (1998) Waxy wheats: Origin, properties, and prospects. Trends Food Sci Technol 9: 135-142
33. Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ (2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164: 731-740
34. He ZH, Rajaram S, Xin ZY, Huang GZ (2001) A History of Wheat Breeding in China. CIMMYT, Mexico, DF
35. Henikoff S, Till BJ, Comai L (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiol 135: 630-636
36. Himelblau E, Gilchrist EJ, Buono K, Bizzell C, Mentzer L, Vogelzang R, Osborn T, Amasino RM, Parkin IA, Haughn GW (2009) Forward and reverse genetics of rapid-cycling Brassica oleracea. Theor Appl Genet 118: 953-961
37. Hsing YI, Chern CG, Fan MJ, Lu PC, Chen KT, Lo SF, Sun PK, Ho SL, Lee KW, Wang YC, Huang WL, Ko SS, Chen S, Chen JL, Chung CI, Lin YC, Hour AL, Wang YW, Chang YC, Tsai MW, Lin YS, Chen YC, Yen HM, Li CP, Wey CK, Tseng CS, Lai MH, Huang SC, Chen LJ, Yu SM (2007) A rice gene activation/knockout mutant resource for high throughput functional genomics. Plant Mol Biol 63: 351-364
38. Hu J, Mitchum MG, Barnaby N, Ayele BT, Ogawa M, Nam E, Lai WC, Hanada A, Alonso JM, Ecker JR, Swain SM, Yamaguchi S, Kamiya Y, Sun TP (2008) Potential sites of bioactive gibberellin production during reproductive growth in Arabidopsis. Plant Cell 20: 320-336
39. International Barley Genome Sequencing C, Mayer KF, Waugh R, Brown JW, Schulman A, Langridge P, Platzer M, Fincher GB, Muehlbauer GJ, Sato K, Close TJ, Wise RP, Stein N (2012) A physical, genetic and functional sequence assembly of the barley genome. Nature 491: 711-716
40. International Rice Genome Sequencing P (2005) The map-based sequence of the rice genome. Nature 436: 793-800
41. Javot H, Penmetsa RV, Terzaghi N, Cook DR, Harrison MJ (2007) A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci USA 104: 1720-1725
42. Jeon JS, Lee S, Jung KH, Jun SH, Jeong DH, Lee J, Kim C, Jang S, Yang K, Nam J, An K, Han MJ, Sung RJ, Choi HS, Yu JH, Choi JH, Cho SY, Cha SS, Kim SI, An G (2000) T-DNA insertional mutagenesis for functional genomics in rice. Plant J 22: 561-570
43. Kadaru SB, Yadav AS, Fjellstrom RG, Oard JH (2006) Alternative EcoTILLING protocol for rapid, cost-effective single-nucleotide polymorphism discovery and genotyping in rice (Oryza sativa L.). Plant Mol Biol Rep 24: 3-22
44. Knoll JE, Ramos ML, Zeng Y, Holbrook CC, Chow M, Chen S, Maleki S, Bhattacharya A, Ozias-Akins P (2011) TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.). BMC Plant Biol 11: 81
45. Kolesnik T, Szeverenyi I, Bachmann D, Kumar CS, Jiang S, Ramamoorthy R, Cai M, Ma ZG, Sundaresan V, Ramachandran S (2004) Establishing an efficient Ac/Ds tagging system in rice: Large-scale analysis of Ds flanking sequences. Plant J 31: 301-314
46. Kumar AP, Boualem A, Bhattacharya A, Parikh S, Desai N, Zambelli A, Leon A, Chatterjee M, Bendahmane A (2013) SMART-Sunflower mutant population and reverse genetic tool for crop improvement. BMC Plant Biol 13: 38
47. Kuntz M (2012) Destruction of public and governmental experiments of GMO in Europe. GM Crops Food 3: 258-264
48. Kurowska M, Daszkowska-Golec A, Gruszka D, Marzec M, Szurman M, Szarejko I, Maluszynski M (2011) TILLING: A shortcut in functional genomics. J Appl Genet 52: 371-390
49. Lababidi S, Mejlhede N, Rasmussen SK, Backes G, Al-Said W, Baum M, Jahoor A (2009) Identification of barley mutants in the cultivar 'Lux' at the Dhnloci through TILLING. Plant Breed 128: 332-336
50. Li YD, Chu ZZ, Liu XG, Jing HC, Liu YG, Hao DY (2010) A cost-effective high-resolution melting approach using the EvaGreen dye for DNA polymorphism detection and genotyping in plants. J Integr Plant Biol 52: 1036-1042
51. Lochlainn SO, Amoah S, Graham NS, Alamer K, Rios JJ, Kurup S, Stoute A, Hammond JP, Ostergaard L, King GJ, White PJ, Broadley MR (2011) High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes. Plant Methods 7: 43
52. MacKey J (1954) Mutation breeding in polyploid cereals. Acta Agr Scand 4: 549-557
53. Mba C (2013) Induced mutations unleash the potentials of plant genetic resources for food and agriculture. Agronomy 3: 200-231
54. McCallum CM, Comai L, Greene EA, Henikoff S (2000a) Targeted screening for induced mutations. Nat Biotechnol 18: 455-457
55. McCallum CM, Comai L, Greene EA, Henikoff S (2000b) Targeting Induced Local Lesions IN Genomes (TILLING) for plant functional genomics. Plant Physiol 123: 439-442
56. Mejlhede N, Kyjovska Z, Backes G, Burhenne K, Rasmussen SK, Jahoor A (2006) EcoTILLING for the identification of allelic variation within the powdery mildew resistance genes mlo and Mla of barley. Plant Breed 125: 461-467
57. Menda N, Semel Y, Peled D, Eshed Y, Zamir D (2004) In silico screening of a saturated mutation library of tomato. Plant J 38: 861-872
58. Minoia S, Petrozza A, D'Onofrio O, Piron F, Mosca G, Sozio G, Cellini F, Bendahmane A, Carriero F (2010) A new mutant genetic resource for tomato crop improvement by TILLING technology. BMC Res Notes 3: 69
59. Missirian V, Comai L, Filkov V (2011) Statistical mutation calling from sequenced overlapping DNA pools in TILLING experiments. BMC Bioinf 12: 287
60. Muth J, Hartje S, Twyman RM, Hofferbert HR, Tacke E, Prufer D (2008) Precision breeding for novel starch variants in potato. Plant Biotechnol J 6: 576-584
61. Negrao S, Almadanim MC, Pires IS, Abreu IA, Maroco J, Courtois B, Gregorio GB, McNally KL, Oliveira MM (2013) New allelic variants found in key rice salt-tolerance genes: An association study. Plant Biotechnol J 11: 87-100
62. Nieto C, Piron F, Dalmais M, Marco CF, Moriones E, Gomez-Guillamon ML, Truniger V, Gomez P, Garcia-Mas J, Aranda MA, Bendahmane A (2007) EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility. BMC Plant Biol 7: 34
63. Okabe Y, Ariizumi T, Ezura H (2013) Updating the Micro-Tom TILLING platform. Breed Sci 63: 42-48
64. Okabe Y, Asamizu E, Ariizumi T, Shirasawa K, Tabata S, Ezura H (2012) Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life. Breed Sci 62: 202-208
65. Okabe Y, Asamizu E, Saito T, Matsukura C, Ariizumi T, Bres C, Rothan C, Mizoguchi T, Ezura H (2011) Tomato TILLING technology: Development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries. Plant Cell Physiol 52: 1994-2005
66. Oleykowski CA, Mullins CRB, Godwin AK, Yeung AT (1998) Mutation detection using a novel plant endonuclease. Nucl Acids Res 26: 4597-4602
67. Parry MA, Madgwick PJ, Bayon C, Tearall K, Hernandez-Lopez A, Baudo M, Rakszegi M, Hamada W, Al-Yassin A, Ouabbou H, Labhilili M, Phillips AL (2009) Mutation discovery for crop improvement. J Exp Bot 60: 2817-2825
68. Perry J, Brachmann A, Welham T, Binder A, Charpentier M, Groth M, Haage K, Markmann K, Wang TL, Parniske M (2009) TILLING in Lotus japonicus identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles toward glycine replacements. Plant Physiol 151: 1281-1291
69. Perry JA, Wang TL, Welham TJ, Gardner S, Pike JM, Yoshida S, Parniske M (2003) A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. Plant Physiol 131: 866-871
70. Piron F, Nicolai M, Minoia S, Piednoir E, Moretti A, Salgues A, Zamir D, Caranta C, Bendahmane A (2010) An induced mutation in tomato eIF4E leads to immunity to two potyviruses. PLoS ONE 5: e11313
71. Raghavan C, Naredo MEB, Wang H, Atienza G, Liu B, Qiu F, McNally KL, Leung H (2006) Rapid method for detecting SNPs on agarose gels and its application in candidate gene mapping. Mol Breed 19: 87-101
72. Rawat N, Sehgal SK, Joshi A, Rothe N, Wilson DL, McGraw N, Vadlani PV, Li W, Gill BS (2012) A diploid wheat TILLING resource for wheat functional genomics. BMC Plant Biol 12: 205
73. Rigola D, van Oeveren J, Janssen A, Bonne A, Schneiders H, van der Poel HJ, van Orsouw NJ, Hogers RC, de Both MT, van Eijk MJ (2009) High-throughput detection of induced mutations and natural variation using KeyPoint technology. PLoS ONE 4: e4761
74. Ririe KM, Rasmussen RP, Wittwer CT (1997) Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 245: 154-160
75. Ronceret A, Doutriaux MP, Golubovskaya IN, Pawlowski WP (2009) PHS1 regulates meiotic recombination and homologous chromosome pairing by controlling the transport of RAD50 to the nucleus. Proc Natl Acad Sci USA 106: 20121-20126
76. Rothe N (2010) Validation of TILLING Populations in Diploid and Hexaploid Wheat. MS thesis, Kansas State University. Available on-line: http://krex.k-state.edu
77. Sabetta W, Alba V, Blanco A, Montemurro C (2011) sunTILL: A TILLING resource for gene function analysis in sunflower. Plant Methods 7: 20
78. Sato Y, Shirasawa K, Takahashi Y, Nishimura M, Nishio T (2006) Mutant selection from progeny of gamma-ray-irradiated rice by DNA heteroduplex cleavage using Brassica petiole extract. Breed Sci 56: 179-183
79. Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463: 178-183
80. Sestili F, Botticella E, Bedo Z, Phillips A, Lafiandra D (2009) Production of novel allelic variation for genes involved in starch biosynthesis through mutagenesis. Mol Breed 25: 145-154
81. Sikora P, Chawade A, Larsson M, Olsson J, Olsson O (2011) Mutagenesis as a tool in plant genetics, functional genomics, and breeding. Int J Plant Genomics 2011: 314829
82. Slade AJ, Fuerstenberg SI, Loeffler D, Steine MN, Facciotti D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23: 75-81
83. Slade AJ, McGuire C, Loeffler D, Mullenberg J, Skinner W, Fazio G, Holm A, Brandt KM, Steine MN, Goodstal JF, Knauf VC (2012) Development of high amylose wheat through TILLING. BMC Plant Biol 12: 69
84. Stemple DK (2004) TILLING-a high-throughput harvest for functional genomics. Nat Rev Genet 5: 1-6
85. Stephenson P, Baker D, Girin T, Perez A, Amoah S, King GJ, Ostergaard L (2010) A rich TILLING resource for studying gene function in Brassica rapa. BMC Plant Biol 10: 62
86. Suzuki T, Eiguchi M, Kumamaru T, Satoh H, Matsusaka H, Moriguchi K, Nagato Y, Kurata N (2008) MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice. Mol Genet Genomics 279: 213-223
87. Tadele Z, Mba C, Till BJ (2010) TILLING for mutations in model plants and crops. In: Jain SM, Brar DS, eds. Molecular Techniques in Crop Improvement. Springer, Netherlands, Berlin. pp. 307-332
88. Talame V, Bovina R, Sanguineti MC, Tuberosa R, Lundqvist U, Salvi S (2008) TILLMore, a resource for the discovery of chemically induced mutants in barley. Plant Biotechnol J 6: 477-485
89. Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327: 818-822
90. Till BJ, Burtner C, Comai L, Henikoff S (2004a) Mismatch cleavage by single-strand specific nucleases. Nucl Acids Res 32: 2632-2641
91. Till BJ, Colbert T, Tompa R, Enns LC, Codomo CA, Johnson JE, Reynolds SH, Henikoff JG, Greene EA, Steine MN, Comai L, Henikoff S (2003a) High-throughput TILLING for functional genomics. Methods Mol Biol 236: 205-220
92. Till BJ, Cooper J, Tai TH, Colowit P, Greene EA, Henikoff S, Comai L (2007) Discovery of chemically induced mutations in rice by TILLING. BMC Plant Biol 7: 19
93. Till BJ, Reynolds SH, Greene EA, Codomo CA, Enns LC, Johnson JE, Burtner C, Odden AR, Young K, Taylor NE, Henikoff JG, Comai L, Henikoff S (2003b) Large-scale discovery of induced point mutations with high-throughput TILLING. Genome Res 13: 524-530
94. Till BJ, Reynolds SH, Weil C, Springer N, Burtner C, Young K, Bowers E, Codomo CA, Enns LC, Odden AR, Greene EA, Comai L, Henikoff S (2004b) Discovery of induced point mutations in maize genes by TILLING. BMC Plant Biol 4: 12
95. Till BJ, Zerr T, Comai L, Henikoff S (2006) A protocol for TILLING and EcoTILLING in plants and animals. Nat Protoc 1: 2465-2477
96. Triques K, Piednoir E, Dalmais M, Schmidt J, Le Signor C, Sharkey M, Caboche M, Sturbois B, Bendahmane A (2008) Mutation detection using END O1: Application to disease diagnostics in humans and TILLING and Eco-TILLING in plants. BMC Mol Biol 9: 42
97. Triques K, Sturbois B, Gallais S, Dalmais M, Chauvin S, Clepet C, Aubourg S, Rameau C, Caboche M, Bendahmane A (2007) Characterization of Arabidopsis thaliana mismatch specific endonucleases: Application to mutation discovery by TILLING in pea. Plant J 51: 1116-1125
98. Tsai H, Howell T, Nitcher R, Missirian V, Watson B, Ngo KJ, Lieberman M, Fass J, Uauy C, Tran RK, Khan AA, Filkov V, Tai TH, Dubcovsky J, Comai L (2011) Discovery of rare mutations in populations: TILLING by sequencing. Plant Physiol 156: 1257-1268
99. Uauy C, Krasileva K, Bailey PC, Buffalo V, Phillips AL, Ayling S, Dubcovsky J (2013) An in-silico functional genomics resource: Targeted re-sequencing of wheat TILLING mutant populations. Plant and Animal Genome XXI Conference. Available on-line: https://pag.confex.com/pag/xxi/webprogram/Paper6334.html
100. Uauy C, Paraiso F, Colasuonno P, Tran RK, Tsai H, Berardi S, Comai L, Dubcovsky J (2009) A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biol 9: 115
101. Voskarides K, Deltas C (2009) Screening for mutations in kidney-related genes using SURVEYOR nuclease for cleavage at heteroduplex mismatches. J Mol Diagn 11: 311-318
102. Wang GX, Tan MK, Rakshit S, Saitoh H, Terauchi R, Imaizumi T, Ohsako T, Tominaga T (2007) Discovery of single-nucleotide mutations in acetolactate synthase genes by EcoTILLING. Pestic Biochem Physiol 88: 143-148
103. Wang J, Sun J, Liu D, Yang W, Wang D, Tong Y, Zhang A (2008a) Analysis of Pina and Pinb alleles in the micro-core collections of Chinese wheat germplasm by EcoTILLING and identification of a novel Pinb allele. In: Rudi Appels, Russell Eastwood, Evans Lagudah, Peter Langridge, Michael Mackay, eds. The 11th International Wheat Genetics Symposium Proceedings. Sydney University Press, Lynne Sydney. ISBN: 978-1-920899-14-1
104. Wang N, Shi L, Tian F, Ning H, Wu X, Long Y, Meng J (2010) Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents. BMC Plant Biol 10: 137
105. Wang N, Wang Y, Tian F, King GJ, Zhang C, Long Y, Shi L, Meng J (2008b) A functional genomics resource for Brassica napus: Development of an EMS mutagenized population and discovery of FAE1 point mutations by TILLING. New Phytol 180: 751-765
106. Wang TL, Uauy C, Robson F, Till B (2012) TILLING in extremis. Plant Biotechnol J 10: 761-772
107. Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM, Nguyen JT, Sato S, Wang ZY, Xia Y, Dixon RA, Harrison MJ, Lamb CJ, Yanofsky MF, Chory J (2000) Activation tagging in Arabidopsis. Plant Physiol 122: 1003-1013
108. Weil CF (2009) TILLING in grass species. Plant Physiol 149: 158-164
109. Wilde HD, Chen Y, Jiang P, Bhattacharya A (2012) Targeted mutation breeding of horticultural plants. Emir J Food Agric 24: 31-41
110. Wu JL, Wu C, Lei C, Baraoidan M, Bordeos A, Madamba MR, Ramos-Pamplona M, Mauleon R, Portugal A, Ulat VJ, Bruskiewich R, Wang G, Leach J, Khush G, Leung H (2005) Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol 59: 85-97
111. Xia Y, Li R, Ning Z, Bai G, Siddique KHM, Yan GX, Baum M, Varshney RK, Guo P (2013) Single nucleotide polymorphisms in HSP17.8 and their association with agronomic traits in barley. PLoS ONE 8: e56816
112. Xia Z, Watanabe S, Yamada T, Tsubokura Y, Nakashima H, Zhai H, Anai T, Sato S, Yamazaki T, Lu S, Wu H, Tabata S, Harada K (2012) Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering. Proc Natl Acad Sci USA 109: E2155-E2164
113. Xin Z, Wang ML, Barkley NA, Burow G, Franks C, Pederson G, Burke J (2008) Applying genotyping (TILLING) and phenotyping analyses to elucidate gene function in a chemically induced sorghum mutant population. BMC Plant Biol 8: 103
114. Yu S, Liao F, Wang F, Wen W, Li J, Mei H, Luo L (2012) Identification of rice transcription factors associated with drought tolerance using the EcoTILLING method. PLoS ONE 7: e30765
115. Zamir D (2001) Improving plant breeding with exotic genetic libraries. Nat Rev Genet 2: 983-989
116. Zhang J, Guo D, Chang Y, You C, Li X, Dai X, Weng Q, Zhang J, Chen G, Li X, Liu H, Han B, Zhang Q, Wu C (2007) Non-random distribution of T-DNA insertions at various levels of the genome hierarchy as revealed by analyzing 13 804 T-DNA flanking sequences from an enhancer-trap mutant library. Plant J 49: 947-959
117. Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B, Wittwer CT (2004) High-resolution DNA melting curve analysis to establish HLA genotypic identity. Tissue Antigens 64: 156-164
118. Zhu Q, Smith SM, Ayele M, Yang L, Jogi A, Chaluvadi SR, Bennetzen JL (2012) High-throughput discovery of mutations in tef semi-dwarfing genes by next-generation sequencing analysis. Genetics 192: 819-829