Antioxidant and DNA protective effects of NTX, a proprietary glycyrrhizin/D-mannitol product, in association with alcohol consumption: A randomized, placebo-controlled, double-blind, crossover study

Journal of Functional Foods

Volumn 34, Issue , 2017, Pages 28-35

  Chigurupati, Harsha ^a   Auddy, Biswajit ^a   Biyani, Manish ^a   Chakrabarti, Shrabana ^a   Pandit, Srikanta ^b   Biswas, Tuhin K ^b   Mondal, Tanmoy ^c   Stohs, Sidney J ^d  

^a Chigurupati Technologies Private Limited   (India)

^b MEDICAL COLLEGE AND HOSPITAL   (India)

^c MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY   (India)

^d CREIGHTON UNIVERSITY   (United States)

Author keywords

Alcohol; D mannitol; DNA damage; Glycyrrhizin; Protein carbonyls; Reactive oxygen species

Indexed keywords

EID: 85017523743     PISSN: 17564646     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jff.2017.04.011     Document Type: Article

References (60)

1. Bondy, S.C., Ethanol toxicity and oxidative stress. Toxicology Letters 63 (1992), 231–242.
2. Brooks, P.J., DNA damage, DNA Repair, and alcohol toxicity-A review. Alcohol Clinical and Experimental Research 2 (1997), 1073–1082.
3. Bruha, R., Dvorak, K., Petrtyl, J., Alcoholic liver disease. World Journal of Hepatology 4 (2012), 81–90.
4. Cederbaum, A.I., Microsomal generation of reactive oxygen species and their possible role in alcohol hepatotoxicity. Alcohol and Alcoholism(Suppl 1), 1991, 291–296.
5. Cederbaum, A.I., Introduction serial review: Alcohol, oxidative stress and cell injury. Free Radical Biology and Medicine 31 (2001), 1524–1526.
6. Chigurupati, H., Auddy, B., Biyani, M., Stohs, S.J., Hepatoprotective effects of a proprietary glycyrrhizin product during alcohol consumption: A randomized, double-blind, placebo-controlled, crossover study. Phytotherapy Research. 30 (2016), 1943–1953.
7. Collins, A.R., The comet assay for DNA damage and repair: Principles, applications, and limitations. Molecular Biotechnology 26 (2004), 249–261.
8. Cooke, M.S., Oxidative DNA damage: Mechanisms, mutation and disease. FASEB Journal 17 (2003), 1195–1214.
9. Cosetino, M., Breda, A., Sanguedoice, F., Landman, J., Stolzenburg, J.-U., Verze, P.,.. Villavicencio, H., The use of mannitol in partial and live donor nephrectomy: An international survey. World Journal of Urology 31 (2013), 977–982.
10. Das, S.K., Vasudevan, D.M., Alcohol-induced oxidative stress. Life Sciences 81 (2007), 177–187.
11. Deshpande, N., Kandi, S., Muddeshwar, M., Das, R., Ramana, K.V., A study of biochemical and hematological markers in alcoholic liver cirrhosis. World Journal of Nutrition and Health 2 (2014), 24–27.
12. Dhanda, A.D., Collins, P.L., Immune dysfunction in acute alcoholic hepatitis. World Journal of Gastroenterology 21 (2015), 14–21.
13. Dyck, L.E., Isoenzymes of aldehyde dehydrogenase in human lymphocytes. Alcohol Clinical and Experimental Research 14 (1990), 534–538.
14. England, M.D., Cavarocchi, N.C., O'Brien, I.F., Solis, E., Pluth, I.R., Orszulak, T.A.,.. Schaff, H.V., Influence of antioxidants (mannitol and allopurinol) on oxygen free radical generation during and after cardiopulmonary bypass. Circulation 74 (1996), 134–137.
15. Floyd, R.A., The role of 8-hydroxyguanine in carcinogenesis. Carcinogenesis 11 (1999), 1447–1450.
16. Gharzouli, K., Gharzouli, A., Amira, S., Khennouf, S., Protective effect of mannitol, glucose-fructose-sucrose-maltose mixture, and natural honey hyperosmolar solutions against ethanol-induced gastric mucosal damage in rats. Experimental Toxicology and Pathology 53 (2001), 175–180.
17. Gong, G., Xiang, L., Yuan, L., Hu, L., Wu, W., Cai, L.,.. Dong, H., Protective effect of glycyrrhizin, a direct HMGB1 inhibitor, on focal cerebral ischemia/reperfusion-induced inflammation, oxidative stress, and apoptosis in rats. PLoS ONE, 9, 2014, e89450.
18. Gong, G., Yuan, L.B., Hu, L., Wu, W., Yin, L., Hou, J.L.,.. Zhou, L.S., Glycyrrhizin attenuates rat ischemic spinal cord injury by suppressing inflammatory cytokines and HMGB1. Acta Pharmacologica Sinica 33 (2012), 11–18.
19. Gu, X.J., Xu, J., Ma, B.Y., Chen, G., Gu, P.Y., Wei, D., Hu, W.X., Effect of glycyrrhizin on traumatic brain injury in rats and its mechanism. Chinese Journal of Traumatology 17 (2014), 1–7.
20. Halliwell, B., Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come?. American Journal of Clinical Nutrition 72 (2000), 1082–1087.
21. Han, K.H., Hashimoto, N., Fukushima, M., Relationships among alcoholic liver disease, antioxidants, and antioxidant enzymes. World Journal of Gastroenterology 21 (2016), 37–49.
22. Hansen, R.E., Roth, D., Winther, J.R., Quantifying the global cellular thiol-disulfide status. Proceedings of the National Academy of Sciences USA 106 (2009), 422–427.
23. Hou, S., Zheng, F., Li, Y., Gao, L., Zhang, J., The protective effect of glycyrrhizic acid on renal tubular epithelial cell injury induced by high glucose. International Journal of Molecular Sciences 15 (2014), 15026–15043.
24. Hsiang, C.Y., Lin, L.J., Kao, S.T., Lo, H.Y., Chou, S.T., Ho, T.Y., Glycyrrhizin, silymarin, and ursodeoxycholic acid regulate a common hepatoprotective pathway in HepG2 cells. Phytomedicine 22 (2015), 768–777.
25. Hsu, T., Huang, K.M., Tsai, H.T., Sung, S.T., Ho, T.N., Cadmium (Cd)-induced oxidative stress down-regulates expression of DNA mismatch recognition proteins MutS homolog 2 (MSH2) and MSH6 in zebrafish (Danio rerio). Aquatic Toxicology 126 (2013), 6–16.
26. Ishida, T., Mizushina, Y., Yagi, S., Irino, Y., Nishiumi, S., Miki, I.,.. Yoshida, M., Inhibitory effects of glycyrrhetinic acid on DNA polymerase and inflammatory activities. Evidenced Based Complementary and Alternative Medicine, 2012, 2012, 650514.
27. Izzo, A.A., Hoon-Kim, S., Radhakrishnan, R., Williamson, E.M., A critical approach to evaluating clinical efficacy, adverse events and drug interactions of herbal remedies. Phytotherapy Research 30 (2016), 691–700.
28. Jung, J.C., Lee, Y.H., Kim, S.H., Kim, K.J., Kim, K.M., Oh, S., Jung, Y.S., Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease. BMC Complementary and Alternative Medicine, 16, 2016, 19, 10.1186/s12906-0997-0.
29. Khan, M.A., Mustafa, J., Musarrat, J., Mechanism of DNA strand breakage induced by photosensitized tetracycline-Cu (II) complex. Mutation Research 525 (2003), 109–119.
30. Kido, R., Sato, I., Tsuda, S., Detection of in vivo DNA damage induced by ethanol in multiple organs of pregnant mice using the alkaline single cell gel electrophoresis (comet assay). Public Health 5 (2006), 41–47.
31. Kim, H.J., Lee, H.K., Cho, J.H., Factor analysis of the biochemical markers related to liver fibrosis. Pakistani Journal of Medical Science 31 (2015), 1043–1046.
32. Kim, M.S., Ong, M., Qu, X., Optimal management for alcoholic liver disease: conventional medications, natural therapy or combination?. World Journal of Gastroenterology 22 (2016), 8–23.
33. Knecht, K.T., Bradford, B.U., Mason, R.P., Thurman, R.G., In vivo formation of a free radical metabolite of ethanol. Molecular Pharmacology 38 (1990), 26–30.
34. Lauterburg, B.H., Velez, M.E., Glutathione deficiency in alcoholics: risk factor for paracetamol hepatotoxicity. Gut 29 (1988), 1153–1157.
35. Li, S., Tan, H.Y., Wang, N., Zhang, Z.J., Lao, L., Wong, C.W., Feng, Y., The role of oxidative stress and antioxidants in liver diseases. International Journal of Molecular Science 16 (2015), 26087–26124.
36. Lieber, C.S., Cytochrome P4502E1: Its physiological and pathological role. Physiology Review 77 (1997), 517–544.
37. Lieber, C.S., Hepatic and other medical disorders of alcoholism: From pathogenesis to treatment. Journal of Studies on Alcohol 591 (1998), 9–25.
38. Lierena, S., Arias-Loste, T., Puente, A., Cabezas, J., Crespo, J., Fabrega, E., Binge drinking: Burden of liver disease and beyond. World Journal of Hepatology 7 (2015), 2703–2715.
39. Lu, F., Xiao, J., Lin, P.Y., Yang, G.J., Convolution spectrometric determination of the effect of hydroxyl radical scavenger on UV radiation-induced DNA aberrance. Guang Pu Xue Yu Guang Fen Xi 24 (2004), 82–84 In Chinese.
40. Mantle, D., Preedy, V.R., Free radicals as mediators of alcohol toxicity. Advances in Drug Reactions and Toxicology Reviews 18 (1999), 235–252.
41. Mathurin, P., Lucey, M.R., Management of alcoholic hepatitis. Journal of Hepatology 56:Suppl 1 (2012), S39–S45.
42. Mira, L., Maia, L., Barreira, L., Manso, C.F., Evidence for radical generation due to NADH oxidation by aldehyde oxidase during ethanol metabolism. Archives of Biochemistry and Biophysics 318 (1995), 53–58.
43. O'Shea, R.S., Dasarathy, S., McCullough, A.J., Alcoholic liver disease. Americal Journal of Gastroenterology 105 (2010), 14–33.
44. Obrenovic, M.E., Li, Y., Parvathaneni, K., Yendluri, B.B., Palacios, H.H., Leszek, J., Aliev, G., Antioxidants in health, disease and aging. CNS Neurological Disorders and Drug Targets 10 (2011), 192–207.
45. Patel, T.K., Williamson, J.D., Mannitol in plants, fungi, and plant-fungal interactions. Trends in Plant Sciences 21 (2016), 486–497.
46. Pejchal, J., Vasieleva, V., Hristozova, M., Vilasova, Z., Vavrova, J., Alyakov, M.,.. Dresler, J., Cytokinesis-block micronucleus (cbmn) assay/cbmn cytome assay in human lymphocytes after in vitro irradiation and its use in Biodosimetry. Military Medical Science Letter (Voj. Zdrav. Listy) 80 (2011), 28–37.
47. Pierson, J.L., Mitchell, M.C., Increased hepatic efflux of glutathione after chronic ethanol feeding. Biochemical Pharmacology 35 (1986), 1533–1537.
48. Pisoschi, A.M., Pop, A., The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry 97 (2015), 55–74.
49. Quan, Z.Y., Ming, Y., Hong, Z.X., Min, L.H., Relationship between DDR and alcoholic liver fibrosis and responses to compound glycyrrhizin tablets on them: An experimental study. Journal of Shandong University 47 (2009), 80–84.
50. Qureshi, M.S., Ahmad, R., Rizwan, R., Malik, A., Manan, A., Qazi, M.H., Shaheen, S., Alcoholic liver disease. Implications of oxidative stress. Professional Medical Journal 22 (2015), 896–903.
51. Sarker, A.H., Watanabe, S., Seki, S., Akiyama, T., Okada, S., Oxygen radical-induced single-strand DNA breaks and repair of the damage in a cell-free system. Mutation Research 337 (1995), 85–95.
52. Shah, D.M., Bock, D.E.M., Darling, R.C., Chang, B.B., Kupinski, A.M., Leather, R.P., Beneficial effects of hypertonic mannitol in acute ischemia-reperfusion injuries in humans. Vascular 4 (1996), 97–100.
53. Sil, R., Ray, D., Chakraborti, A.S., Glycyrrhizin ameliorates metabolic syndrome-induced liver damage in experimental animal model. Molecular and Cellular Biochemistry 409 (2015), 177–189.
54. Tezil, T., Basaga, H., Modulation of cell death in age-related diseases. Current Pharmaceutical Design 20 (2014), 3052–3067.
55. Trotti, R., Carratelli, M., Barbieri, M., Bosone, D., Rondanelli, M., Bo, P., Oxidative stress and a thrombophilic condition in alcoholics without severe liver disease. Haematologica 86 (2001), 85–91.
56. Tsukamoto, H., Lu, S.C., Current concepts in the pathogenesis of alcoholic liver injury. FASEB Journal 15 (2001), 1335–1349.
57. Wang, J., Guo, J., Liu, S., Inhibitory effect of glycyrrhizin on NF-kappa B binding activity in CCl4 plus ethanol induced liver cirrhosis in rats. Zhonghue Gan Zang Bin Za Zhi 7 (1999), 42–43.
58. Wu, D., Cederbaum, A.I., Alcohol, oxidative stress and free radical damage. Alcohol Research and Health 27 (2003), 277–284.
59. Zhao, J., Xu, S., Song, F., Nian, L., Zhou, X., Wang, S., 2,3,5,4-Tetrahydroxystilbene-2-O-β-D-glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine-induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase. IUBMB Life 66 (2014), 711–722.
60. Zou, J.Y., Crews, F.T., Release of neuronal HMGB1 by ethanol through decreased HDAC activity activates brain neuroimmune signalling. PLoS ONE, 14, 2014, es87915, 10.1371/jopurnal.pone.0087915.