Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Sequeira, Ana Filipa ^a,b   Turchetto, Jeremy ^c   Saez, Natalie J ^c,d   Peysson, Fanny ^c   Ramond, Laurie ^c   Duhoo, Yoan ^c   Blémont, Marilyne ^c   Fernandes, Vânia O ^a   Gama, Luís T ^a   Ferreira, Luís M A ^a,b   Guerreiro, Catarina I P I ^b   Gilles, Nicolas ^e   Darbon, Hervé ^c   Fontes, Carlos M G A ^a,b   Vincentelli, Renaud ^c  

^a FACULDADE DE MEDICINA VETERINÁRIA   (Portugal)

^b NZYTech Genes and Enzymes   (Portugal)

^c CNRS   (France)

^d UNIVERSITY OF QUEENSLAND   (Australia)

^e SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

Author keywords

Disulphide rich peptides; Escherichia coli (E. coli); Fusion protein; Gene design; High throughput expression; Periplasm; Recombinant expression; Venom peptides

Indexed keywords

CYSTEINE; DISULFIDE; DITHIOTHREITOL; HYBRID PROTEIN; PEPTIDE; PROLINE; PROTEINASE; TEV PROTEASE; VENOM;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM CULTURE; CARBOXY TERMINAL SEQUENCE; CODON USAGE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; GENE FUSION; HIGH THROUGHPUT SEQUENCING; MOLECULAR RECOGNITION; NONHUMAN; OXIDATION KINETICS; OXIDATION REDUCTION REACTION; PERIPLASM; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RESIDUE ANALYSIS; SOLUBILITY; ANIMAL; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMISTRY; GENE VECTOR; GENETICS; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PEPTIDE SYNTHESIS; PROCEDURES;

ANIMALS; BIOTECHNOLOGY; CLONING, MOLECULAR; DISULFIDES; ENDOPEPTIDASES; ESCHERICHIA COLI; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; OXIDATION-REDUCTION; PEPTIDE BIOSYNTHESIS; PEPTIDES; PERIPLASM; RECOMBINANT FUSION PROTEINS; SOLUBILITY; VENOMS;

EID: 85009962354     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0618-0     Document Type: Article

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