Functional expression of spider neurotoxic peptide Huwentoxin-I in E. coli

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Meng, Er ^a,b   Cai, Tian Fu ^a   Li, Wen Ying ^b   Zhang, Hui ^a   Liu, Yan Bo ^a   Peng, Kuan ^c   Liang, Songping ^a   Zhang, Dong Yi ^b  

^a HUNAN NORMAL UNIVERSITY   (China)

^b NATIONAL UNIVERSITY OF DEFENSE TECHNOLOGY   (China)

^c CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

HUWENTOXIN 1; SPIDER VENOM; UNCLASSIFIED DRUG; HUWENTOXIN I; NEUROTOXIN; PEPTIDE; REPTILIAN PROTEIN; SCN9A PROTEIN, HUMAN; SODIUM CHANNEL;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; EMBRYO; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL ELECTROPHORESIS; GENE EXPRESSION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TOXIN ANALYSIS; AMINO ACID SEQUENCE; ANIMAL; CELL STRAIN HEK293; CHEMICAL STRUCTURE; CHEMISTRY; DRUG EFFECT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SPIDER;

ARANEAE; ORNITHOCTONUS HUWENA;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; HEK293 CELLS; HUMANS; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NEUROTOXINS; PEPTIDES; REPTILIAN PROTEINS; SODIUM CHANNELS; SPIDER VENOMS; SPIDERS;

EID: 79959554744     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0021608     Document Type: Article

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