Probing the structural dynamics of the CRISPR-Cas9 RNA-guided DNA-cleavage system by coarse-grained modeling

Proteins: Structure, Function and Bioinformatics

Volumn 85, Issue 2, 2017, Pages 342-353

  Zheng, Wenjun ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

Cas9; coarse grained modeling; CRISPR; elastic network model; flexibility; hotspot residue; normal mode analysis; transition pathway

Indexed keywords

AMINO ACID; CAS9 ENDONUCLEASE; CRISPR ASSOCIATED PROTEIN; DNA; ENDONUCLEASE; RNA; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; GUIDE RNA; PROTEIN BINDING;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; COARSE GRAINED MODELING; CONFORMATIONAL TRANSITION; CRISPR CAS SYSTEM; DNA CLEAVAGE; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; GENE TARGETING; MATHEMATICAL ANALYSIS; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN RNA BINDING; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; BINDING SITE; CHEMISTRY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE EDITING; GENETICS; METABOLISM; MOLECULAR DYNAMICS; THERMODYNAMICS;

BACTERIAL PROTEINS; BINDING SITES; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA; DNA CLEAVAGE; ENDONUCLEASES; GENE EDITING; MOLECULAR DYNAMICS SIMULATION; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN STRUCTURE, SECONDARY; RNA; RNA, GUIDE; THERMODYNAMICS;

EID: 85008234074     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.25229     Document Type: Article

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