Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions

Nature Biotechnology

Volumn 34, Issue 10, 2016, Pages 1060-1065

  Morita, Sumiyo ^a   Noguchi, Hirofumi ^b   Horii, Takuro ^a   Nakabayashi, Kazuhiko ^c   Kimura, Mika ^a   Okamura, Kohji ^c   Sakai, Atsuhiko ^b   Nakashima, Hideyuki ^b   Hata, Kenichiro ^c   Nakashima, Kinichi ^b   Hatada, Izuho ^a  

^a GUNMA UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c NATIONAL RESEARCH INSTITUTE FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; CELL CULTURE; CELLS; CYTOLOGY; DNA; DNA SEQUENCES; METHYLATION; PEPTIDES; STEM CELLS;

CANCER CELL LINES; CATALYTIC DOMAINS; DNA DEMETHYLATION; DNA METHYLATION; EMBRYONIC STEM CELLS; NEURAL PRECURSORS; REGULATORY REGIONS; SPECIFIC SEQUENCES;

CHEMICAL REACTIONS;

AMINO ACID; CAS9 NUCLEASE; DIOXYGENASE; HYBRID PROTEIN; NUCLEASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; STAT3 PROTEIN; TEN ELEVEN 1 HYDROXYLASE; TRANSCRIPTION FACTOR GCN4; UNCLASSIFIED DRUG; BASIC LEUCINE ZIPPER TRANSCRIPTION FACTOR; CRISPR ASSOCIATED PROTEIN; GCN4 PROTEIN, S CEREVISIAE; MIXED FUNCTION OXIDASE; ONCOPROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; TET1 PROTEIN, HUMAN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CANCER CELL LINE; CELL DIFFERENTIATION; CONTROLLED STUDY; DNA METHYLATION; EMBRYONIC STEM CELL; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FETUS; GENE LOCUS; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; UPREGULATION; ANIMAL; CATALYSIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CPG ISLAND; GENE TARGETING; GENETIC ENGINEERING; GENETICS; PROCEDURES; PROTEIN DOMAIN;

ANIMALS; BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTORS; CATALYSIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CPG ISLANDS; CRISPR-ASSOCIATED PROTEINS; DNA METHYLATION; GENE TARGETING; GENETIC ENGINEERING; MICE; MIXED FUNCTION OXYGENASES; PROTEIN DOMAINS; PROTO-ONCOGENE PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SINGLE-CHAIN ANTIBODIES;

EID: 84992126346     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3658     Document Type: Article

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