Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1

Science

Volumn 324, Issue 5929, 2009, Pages 930-935

  Tahiliani, Mamta ^a   Koh, Kian Peng ^a   Shen, Yinghua ^b   Pastor, William A ^a   Bandukwala, Hozefa ^a   Brudno, Yevgeny ^b   Agarwal, Suneet ^c   Iyer, Lakshminarayan M ^d   Liu, David R ^b   Aravind, L ^d   Rao, Anjana ^a  

^a CENTER FOR BLOOD RESEARCH   (United States)

^b HARVARD UNIVERSITY   (United States)

^c BOSTON CHILDREN S HOSPITAL   (United States)

^d NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 OXOGLUTARIC ACID; 5 HYDROXYMETHYLCYTOSINE; 5 METHYLCYTOSINE; DNA; FERROUS ION; JBP1 PROTEIN; JBP2 PROTEIN; MIXED LINEAGE LEUKEMIA PROTEIN; PROTEIN; PROTEIN DERIVATIVE; RNA; TET1 PROTEIN; THYMINE; UNCLASSIFIED DRUG; 5-HYDROXYMETHYLCYTOSINE; CYTIDYLYL 3' 5' GUANOSINE; CYTIDYLYL-3'-5'-GUANOSINE; CYTOSINE; DINUCLEOSIDE PHOSPHATE; DNA BINDING PROTEIN; DRUG DERIVATIVE; ONCOPROTEIN; TET1 PROTEIN, HUMAN; TET1 PROTEIN, MOUSE;

CYTOLOGY; DNA; ENZYME; MAMMAL; NUCLEIC ACID; OXIDATION;

ACUTE GRANULOCYTIC LEUKEMIA; ANIMAL CELL; ARTICLE; CATALYSIS; CELL CULTURE; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; EPIGENETICS; GENE; GENETIC REGULATION; GENOME; IN VITRO STUDY; MAMMAL; MLL GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; RNA INTERFERENCE; TRYPANOSOMA; AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CHEMISTRY; DNA METHYLATION; GENETIC TRANSFECTION; GENETICS; HUMAN; HYDROXYLATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

MAMMALIA;

5-METHYLCYTOSINE; AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CYTOSINE; DINUCLEOSIDE PHOSPHATES; DNA; DNA METHYLATION; DNA-BINDING PROTEINS; EMBRYONIC STEM CELLS; HUMANS; HYDROXYLATION; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; PROTO-ONCOGENE PROTEINS; RNA INTERFERENCE; SEQUENCE ALIGNMENT; TRANSFECTION;

EID: 66149146320     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1170116     Document Type: Article

References (34)

1. M. EhrLich, R. Y. Wang, Science 212, 1350 (1981).
2. M. G. GoLL, T. H. Bestor, Annu. Rev. Biochem. 74, 481 (2005).
3. W. Reik, Nature 447, 425 (2007).
4. A. Bird, Genes Dev. 16, 6 (2002).
5. E. N. GaL-Yam, Y. Saito, G. Egger, P. A. Jones, Annu. Rev. Med. 59, 267 (2008).
6. P. Borst, R. Sabatini, Annu. Rev. Microbiol. 62, 235 (2008).
7. Z. Yu et al, Nucleic Acids Res. 35, 2107 (2007).
8. L. J. Cliffe et al., Nucleic Acids Res. 37, 1452 (2009).
9. L. Aravind, E. V. Koonin, Genome Biol. 2, RESEARCH0007 (2001).
10. M. D. ALLen et al., EMBO J. 25, 4503 (2006).
11. H. Cedar, A. SoLage, G. GLaser, A. Razin, Nucleic Acids Res. 6, 2125 (1979).
12. G. R. Wyatt, S. S. Cohen, Biochem. J. 55, 774 (1953).
13. L. Que Jr., R. Y. Ho, Chem. Rev. 96, 2607 (1996).
14. C. Loenarz, C. J. Schofield, Nat. Chem. Biol. 4, 152 (2008).
15. L. E. Netto, E. R. Stadtman, Arch. Biochem. Biophys. 333, 233 (1996).
16. M. W. Simmen, Genomics 92, 33 (2008).
17. V. Valinluck et al., Nucleic Acids Res. 32, 4100 (2004).
18. V. Valinluck, L. C. Sowers, Cancer Res. 67, 946 (2007).
19. E. Privat, L. C. Sowers, Chem. Res. Toxicol. 9, 745 (1996).
20. J. G. Flaks, S. S. Cohen, J. Biol. Chem. 234, 1501 (1959).
21. A. H. Alegria, Biochim. Biophys. Acta 149, 317 (1967).
22. S. K. Ooi, T. H. Bestor, Cell 133, 1145 (2008).
23. J. Jiricny, M. Menigatti, Cell 135, 1167 (2008).
24. S. V. Cannon, A. Cummings, G. W. Teebor, Biochem. Biophys. Res. Commun. 151, 1173 (1988).
25. B. Zhu et al., Proc. Natl. Acad. Sci. U.S.A. 97, 5135 (2000).
26. R. Metivier et al., Nature 452, 45 (2008).
27. S. Kangaspeska et al., Nature 452, 112 (2008).
28. K. Rai et al., Cell 135, 1201 (2008).
29. V. Rusmintratip, L. C. Sowers, Proc. Natl. Acad. Sci. U.S.A. 97, 14183 (2000).
30. R. Ono et al., Cancer Res. 62, 4075 (2002).
31. R. B. Lorsbach et al., Leukemia 17, 637 (2003).
32. F. Viguie et al., Leukemia 19, 1411 (2005).
33. F. Delhommeau et al., paper presented at the American Society of Hematology Annual Meeting and Exposition, San Francisco, CA, December 9, 2008.
34. We thank K. Kreuzer for the gift of the T4 phage and E. coli ER1656 and CR63; Charles Richardson, Udi Qimron, and Ben Beauchamp for advice and assistance in culturing T4 phage; Melissa Call for advice on production of recombinant proteins in insect cells; and Patrick Hogan for many helpful discussions. This work was supported by NIH grant AI44432, a Scholar Award from the Juvenile Diabetes Research Foundation, and a Seed grant from the Harvard Stem Cell Institute (to A.R.); Howard Hughes Medical Institute and NIH/NIGMS (R01GM065865) funding (to D.R.L.); an American Heart Association postdoctoral fellowship (to K.P.K.); intramural funds of the National Library of Medicine, NIH (to L.A. and L.I.); a Lady Tata Memorial postdoctoral fellowship (to H.B.); NIH award K08 HL089150 (to S.A.); NSF Graduate Fellowships (to W.A.P. and Y.B.); and a Department of Defense graduate fellowship (to W.A.P.).