Scarless gene tagging with one-step transformation and two-step selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe

PLoS ONE

Volumn 11, Issue 10, 2016, Pages

  Landgraf, Dirk ^a   Huh, Dann ^b   Hallacli, Erinc ^a   Lindquist, Susan ^a,c  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b HARVARD UNIVERSITY   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; RAB PROTEIN; HYBRID PROTEIN; LUMINESCENT AGENT; PHOTOPROTEIN; PRIMER DNA; SACCHAROMYCES CEREVISIAE PROTEIN; SCHIZOSACCHAROMYCES POMBE PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOMATION; CARBOXY TERMINAL SEQUENCE; CELL TRANSFORMATION; CODON; CONTROLLED STUDY; DNA FLANKING REGION; GENE; GENE CASSETTE; GENETIC ANALYSIS; HOMOLOGOUS RECOMBINATION; NONHUMAN; OPEN READING FRAME; PRE1 GENE; RELIABILITY; RSP5 GENE; SACCHAROMYCES CEREVISIAE; SCARLESS GENE TAGGING; SCHIZOSACCHAROMYCES POMBE; FUNGAL GENE; GENE TARGETING; GENETICS; METABOLISM; PROCEDURES; SCHIZOSACCHAROMYCES;

DNA PRIMERS; GENE TARGETING; GENES, FUNGAL; LUMINESCENT AGENTS; LUMINESCENT PROTEINS; OPEN READING FRAMES; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

EID: 84991716883     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0163950     Document Type: Article

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