Avoiding the ends: Internal epitope tagging of proteins using transposon Tn7

Genetics

Volumn 200, Issue 1, 2015, Pages 47-58

  Zordan, Rebecca E ^a   Beliveau, Brian J ^a   Trow, Jonathan A ^a   Craig, Nancy L ^a   Cormack, Brendan P ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Epitope tag; Protein tagging; Saccharomyces; Transposition

Indexed keywords

EPITOPE; FUNGAL PROTEIN; GENOMIC DNA; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; PROTEIN TAG; RESTRICTION ENDONUCLEASE; DCW1 PROTEIN, S CEREVISIAE; MANNOSIDASE; MEMBRANE PROTEIN; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSPOSON;

ALLELE; AMINO ACID SEQUENCE; ARTICLE; CANDIDA GLABRATA; ENZYME DEGRADATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CASSETTE; GENE ISOLATION; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; GENETIC CODE; GENETIC RECOMBINATION; IN VITRO STUDY; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; PROCESSING; PROTEIN EXPRESSION; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; SEQUENCE TAGGED SITE; TRANSPOSON; CANDIDA; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; PROTEIN ENGINEERING;

CANDIDA GLABRATA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; CANDIDA; DNA TRANSPOSABLE ELEMENTS; EPITOPES; MANNOSIDASES; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84929001472     PISSN: 00166731     EISSN: 19432631     Source Type: Journal    
DOI: 10.1534/genetics.114.169482     Document Type: Article

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