Localization of gene products using a chromosomally tagged GFP-fusion library in the fission yeast Schizosaccharomyces pombe

Genes to Cells

Volumn 14, Issue 2, 2009, Pages 217-225

  Hayashi, Aki ^a,c   Da Qiao, Ding ^a   Tsutsumi, Chihiro ^a   Chikashige, Yuji ^a   Masuda, Hirohisa ^a,d   Haraguchi, Tokuko ^a   Hiraoka, Yasushi ^a,b  

^a NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c RIKEN   (Japan)

^d THE FRANCIS CRICK INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; HEMAGGLUTININ; MESSENGER RNA; SCHIZOSACCHAROMYCES POMBE PROTEIN;

ARTICLE; CELL MEMBRANE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CHROMOSOMAL LOCALIZATION; CHROMOSOME; CONTROLLED STUDY; CYTOPLASM; FUNGAL STRAIN; GENE CONSTRUCT; GENE EXPRESSION; GENE LOCATION; MOLECULAR LIBRARY; NONHUMAN; NUCLEOLUS; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN LOCALIZATION; SCHIZOSACCHAROMYCES POMBE; SCREENING; EVALUATION; FUNGAL GENE; GENE LIBRARY; GENETICS; METABOLISM; PRODUCTIVITY; SCHIZOSACCHAROMYCES; SEQUENCE TAGGED SITE; TISSUE DISTRIBUTION;

SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

EFFICIENCY; GENE LIBRARY; GENES, FUNGAL; GREEN FLUORESCENT PROTEINS; HEMAGGLUTININS; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE TAGGED SITES; TISSUE DISTRIBUTION;

EID: 60849132182     PISSN: 13569597     EISSN: 13652443     Source Type: Journal    
DOI: 10.1111/j.1365-2443.2008.01264.x     Document Type: Article

References (19)

1. Bähler, J., Wu, J.Q., Longtine, M.S., Shah, N.G., McKenzie, A. 3rd, Steever, A.B., Wach, A., Philippsen, P. & Pringle, J.R. (1998) Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14, 943-951.
2. Chikashige, Y., Tsutsumi, C., Okamasa, K., Yamane, M., Nakayama, J., Niwa, O., Haraguchi, T. & Hiraoka, Y. (2007) Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe. Cell Struct. Funct. 32, 149-161.
3. Ding, D.Q., Tomita, Y., Yamamoto, A., Chikashige, Y., Haraguchi, T. & Hiraoka, Y. (2000) Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library. Genes Cells 5, 169-190.
4. Drawid, A., Jansen, R. & Gerstein, M. (2000) Genome-wide analysis relating expression level with protein subcellular localization. Trends Genet. 16, 426-430.
5. Ghaemmaghami, S., Huh, W.K., Bower, K., Howson, R.W., Belle, A., Dephoure, N., O'Shea, E.K. & Weissman, J.S. (2003) Global analysis of protein expression in yeast. Nature 425, 737-741.
6. Haraguchi, T., Ding, D.Q., Yamamoto, A., Kaneda, T., Koujin, T. & Hiraoka, Y. (1999) Multiple-color fluorescence imaging of chromosomes and microtubules in living cells. Cell Struct. Funct. 24, 291-298.
7. Hayashi, A., Asakawa, H., Haraguchi, T. & Hiraoka, Y. (2006) Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe. Mol. Biol. Cell 17, 5173-5184.
8. Hayden, M.S., & Byers, B. (1992) Minimal extent of homology required for completion of meiotic recombination in Saccharomyces cerevisiae. Dev. Genet. 13, 498-514.
9. Huh, W.K., Falvo, J.V., Gerke, L.C., Carroll, A.S., Howson, R.W., Weissman, J.S. & O'Shea, E.K. (2003) Global analysis of protein localization in budding yeast. Nature 425, 686-691.
10. Ihmels, J., Friedlander, G., Bergmann, S., Sarig, O., Ziv, Y. & Barkai, N. (2002) Revealing modular organization in the yeast transcriptional network. Nat. Genet. 31, 370-377.
11. Kumar, A., Agarwal, S., Heyman, J.A., Matson, S., Heidtman, M., Piccirillo, S., Umansky, L., Drawid, A., Jansen, R., Liu, Y., Cheung, K.H., Miller, P., Gerstein, M., Roeder, G.S. & Snyder, M. (2002) Subcellular localization of the yeast proteome. Genes Dev. 16, 707-719.
12. Matsumoto, S., Yanagida, M. & Nurse, P. (1987) Histone transcription in cell cycle mutants of fission yeast. EMBO J. 6, 1093-1097.
13. Matsuyama, A., Arai, R., Yashiroda, Y., Shirai, A., Kamata, A., Sekido, S., Kobayashi, Y., Hashimoto, A., Hamamoto, M., Hiraoka, Y., Horinouchi, S. & Yoshida, M. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24, 841-847.
14. Moreno, S., Klar, A. & Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823.
15. Niedenthal, R.K., Riles, L., Johnston, M. & Hegemann, J.H. (1996) Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast 12, 773-786.
16. Ross-Macdonald, P., Coelho, P.S., Roemer, T. et al. (1999) Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 402, 413-418.
17. Sawin, K.E. & Nurse, P. (1996) Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. Proc. Natl Acad. Sci. USA 93, 15146-15151.
18. Simpson, J.C., Wellenreuther, R., Poustka, A., Pepperkok, R. & Wiemann, S. (2000) Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 1, 287-292.
19. Wach, A. (1996) PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12, 259-265.