메뉴 건너뛰기




Volumn 12, Issue 3, 1996, Pages 259-265

PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae

Author keywords

Functional analysis; G418 resistance; PCR based gene disruption; Yeast genome analysis

Indexed keywords

ARTICLE; CONTROLLED STUDY; GENE DISRUPTION; GENETIC ANALYSIS; GENETIC MARKER; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

EID: 0029871347     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19960315)12:3<259::AID-YEA901>3.0.CO;2-C     Document Type: Article
Times cited : (720)

References (17)
  • 1
    • 0028211009 scopus 로고
    • PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates
    • Barnes, W. M. (1994). PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. USA 91, 2216-2220.
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 2216-2220
    • Barnes, W.M.1
  • 2
    • 0027237665 scopus 로고
    • A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
    • Baudin, A., Ozier, K. O., Denouel, A., Lacroute, F. and Cullin, C. (1993). A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21, 3329-3330.
    • (1993) Nucleic Acids Res. , vol.21 , pp. 3329-3330
    • Baudin, A.1    Ozier, K.O.2    Denouel, A.3    Lacroute, F.4    Cullin, C.5
  • 3
    • 0000182975 scopus 로고
    • XLI-Blue: A High Efficiency Plasmid Transforming recA Escherichia coli Strain with β-Galactosidase Selection
    • Bullock, W. O., Fernandez, J. M. and Short, J. M. (1987). XLI-Blue: A High Efficiency Plasmid Transforming recA Escherichia coli Strain with β-Galactosidase Selection. BioTechniques 5, 376-378.
    • (1987) BioTechniques , vol.5 , pp. 376-378
    • Bullock, W.O.1    Fernandez, J.M.2    Short, J.M.3
  • 4
    • 0002819495 scopus 로고
    • High efficiency transformation with lithium acetate
    • Johnston, J. R (Ed.), IRL Press, Oxford, UK
    • Gietz, R. D. and Woods, R. A. (1994). High efficiency transformation with lithium acetate in Johnston, J. R (Ed.), Molecular Genetics of Yeast, A Practical Approach, IRL Press, Oxford, UK, pp. 121-134.
    • (1994) Molecular Genetics of Yeast, A Practical Approach , pp. 121-134
    • Gietz, R.D.1    Woods, R.A.2
  • 5
    • 44949267924 scopus 로고
    • Rapid assessment of S. cerevisiae mating type by PCR
    • Huxley, C., Green, E. D. and Dunham, I. (1990). Rapid assessment of S. cerevisiae mating type by PCR. Trends Genet. 6, 236.
    • (1990) Trends Genet. , vol.6 , pp. 236
    • Huxley, C.1    Green, E.D.2    Dunham, I.3
  • 6
    • 0019131495 scopus 로고
    • Expression of Transposable Antibiotic Resistance Elements in Saccharomyces
    • Jimenez, A. and Davies, J. (1980). Expression of Transposable Antibiotic Resistance Elements in Saccharomyces. Nature 287, 869-871.
    • (1980) Nature , vol.287 , pp. 869-871
    • Jimenez, A.1    Davies, J.2
  • 7
    • 0029096027 scopus 로고
    • A method for performing precise alterations in the yeast genome using a recyclable selectable marker
    • Laengle-Ronault, F. and Jacobs, E. (1995). A method for performing precise alterations in the yeast genome using a recyclable selectable marker. Nucl. Acids Res. 23, 3079-3081.
    • (1995) Nucl. Acids Res. , vol.23 , pp. 3079-3081
    • Laengle-Ronault, F.1    Jacobs, E.2
  • 8
    • 0029120399 scopus 로고
    • Micro-homology mediated PCR-targeting in Saccharomyces cerevisiae
    • Manivasakam, P., Weber, S. C., McElver, J. and Schiestl, R. H. (1995). Micro-homology mediated PCR-targeting in Saccharomyces cerevisiae. Nucl. Acids Res. 23, 2799-2800.
    • (1995) Nucl. Acids Res. , vol.23 , pp. 2799-2800
    • Manivasakam, P.1    Weber, S.C.2    McElver, J.3    Schiestl, R.H.4
  • 9
    • 0019469231 scopus 로고
    • Nucleotide Sequence of the Kanamycin Resistance Transposon Tn903
    • Oka, A., Sugisaki, H. and Takanami, M. (1981). Nucleotide Sequence of the Kanamycin Resistance Transposon Tn903. J. Mol. Biol. 147, 217-226.
    • (1981) J. Mol. Biol. , vol.147 , pp. 217-226
    • Oka, A.1    Sugisaki, H.2    Takanami, M.3
  • 11
    • 0024799254 scopus 로고
    • High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
    • Schiestl, R. H. and Gietz, R. D. (1989). High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16, 339-346.
    • (1989) Curr. Genet. , vol.16 , pp. 339-346
    • Schiestl, R.H.1    Gietz, R.D.2
  • 13
    • 0027972417 scopus 로고
    • Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii
    • Steiner, S. and Philippsen, P. (1994). Sequence and promoter analysis of the highly expressed TEF gene of the filamentous fungus Ashbya gossypii. Mol. Gen Genet. 242, 263-271.
    • (1994) Mol. Gen Genet. , vol.242 , pp. 263-271
    • Steiner, S.1    Philippsen, P.2
  • 14
    • 0001312016 scopus 로고
    • Basic Techniques of Yeast Genetics
    • Ausubel, F. A., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (Eds), Greene Publishing and Wiley-Interscience, New York
    • Treco, D. A. (1989). Basic Techniques of Yeast Genetics. In Current Protocols in Molecular Biology. Ausubel, F. A., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (Eds), pp. 3.1.1-13.1.7, Greene Publishing and Wiley-Interscience, New York.
    • (1989) Current Protocols in Molecular Biology
    • Treco, D.A.1
  • 15
    • 0028198480 scopus 로고
    • Selectable marker replacement in Saccharomyces cerevisiae
    • Vidal, M. and Gaber, R. F. (1994). Selectable marker replacement in Saccharomyces cerevisiae. Yeast 10, 141-149.
    • (1994) Yeast , vol.10 , pp. 141-149
    • Vidal, M.1    Gaber, R.F.2
  • 16
    • 0028676232 scopus 로고
    • New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
    • Wach, A., Brachat, A., Poehlmann, R. and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
    • (1994) Yeast , vol.10 , pp. 1793-1808
    • Wach, A.1    Brachat, A.2    Poehlmann, R.3    Philippsen, P.4
  • 17
    • 0028794516 scopus 로고
    • Construction of a Set of Convenient Saccharomyces cerevisiae Strains that are Isogenic to S288C
    • Winston, F., Dollard, C. and Ricupero-Hovasse, S. L. (1995). Construction of a Set of Convenient Saccharomyces cerevisiae Strains that are Isogenic to S288C. Yeast 11, 53-55.
    • (1995) Yeast , vol.11 , pp. 53-55
    • Winston, F.1    Dollard, C.2    Ricupero-Hovasse, S.L.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.