Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae

Yeast

Volumn 14, Issue 10, 1998, Pages 953-961

  Longtine, Mark S ^a   McKenzie III, Amos ^a   Demarini, Douglas J ^a,c   Shah, Nirav G ^a   Wach, Achim ^b,d   Brachat, Arndt ^b   Philippsen, Peter ^b   Pringle, John R ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF BASEL   (Switzerland)

^c GLAXOSMITHKLINE   (United Kingdom)

^d Bureco AG   (Switzerland)

Author keywords

Epitope tagging; Functional analysis; Gene deletion; Gene truncation; Green fluorescent protein; Overexpression studies; Polymerase chain reaction

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ARTICLE; CONTROLLED STUDY; ECONOMIC ASPECT; ESCHERICHIA COLI; GENE DELETION; GENE FUNCTION; GENE OVEREXPRESSION; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

DNA PRIMERS; GENE DELETION; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MOLECULAR BIOLOGY; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 0031820288     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U     Document Type: Article

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