A PCR-based strategy to generate yeast strains expressing endogenous levels of amino-terminal epitope-tagged proteins

Biotechnology Journal

Volumn 3, Issue 4, 2008, Pages 524-529

  Booher, Keith R ^a   Kaiser, Peter ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Epitope tagging; N terminal; Yeast

Indexed keywords

GENES; NUCLEOTIDES; PROTEINS;

CELLULAR PROTEIN; EPITOPE-TAGGING; GENE OF INTEREST; N-TERMINAL; YEAST STRAIN;

YEAST;

EPITOPE; ESSENTIAL AMINO ACID; FUNGAL DNA; NONESSENTIAL AMINO ACID; NUCLEOTIDE DERIVATIVE;

AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; DIPLOIDY; DNA DETERMINATION; ESSENTIAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; GENE LOCUS; GENETIC CODE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SEQUENCE TAGGED SITE;

EID: 42949176714     PISSN: 18606768     EISSN: None     Source Type: Journal    
DOI: 10.1002/biot.200800012     Document Type: Article

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