High-efficiency gene targeting in Schizosaccharomyces pombe using a modular, PCR-based approach with long tracts of flanking homology

Yeast

Volumn 15, Issue 13, 1999, Pages 1419-1427

  Krawchuk, Michelle D ^a   Wahls, Wayne P ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Epitope tagging; Fission yeast; Gene disruption; Gene expression; Homologous recombination; Polymerase chain reaction; Targeted integration

Indexed keywords

EPITOPE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; DELETION MUTANT; DNA FLANKING REGION; GENE TARGETING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY; SILENT GENE; YEAST;

CHROMOSOME MAPPING; GENE TARGETING; POLYMERASE CHAIN REACTION; SCHIZOSACCHAROMYCES;

EID: 0033619113     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19990930)15:13<1419::AID-YEA466>3.0.CO;2-Q     Document Type: Article

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