Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

Developmental Biology

Volumn 418, Issue 1, 2016, Pages 1-9

  Hashimoto, Masakazu ^a   Yamashita, Yukiko ^b   Takemoto, Tatsuya ^b  

^a OSAKA UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKUSHIMA   (Japan)

Author keywords

Cas9 protein; CRISPR Cas9 system; Electroporation; Genome editing

Indexed keywords

CAS9 PROTEIN; CRISPR ASSOCIATED PROTEIN; GENOMIC DNA; MESSENGER RNA; PROTEIN; UNCLASSIFIED DRUG; ENDONUCLEASE; GUIDE RNA;

ALLELE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA END JOINING REPAIR; DNA REPAIR; ELECTROPORATION; EMBRYO; EMBRYO DEVELOPMENT; GENE MUTATION; GENE REPLICATION; HOMOLOGOUS RECOMBINATION; IN VITRO FERTILIZATION; MOSAICISM; MOUSE; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; TRANSGENIC MOUSE; ZYGOTE; ANIMAL; C57BL MOUSE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; CYTOLOGY; DBA MOUSE; EMBRYOLOGY; GENE EDITING; GENE TARGETING; GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES;

ANIMALS; BASE SEQUENCE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; ELECTROPORATION; ENDONUCLEASES; GENE EDITING; GENE KNOCK-IN TECHNIQUES; MICE; MICE, INBRED C57BL; MICE, INBRED DBA; MOSAICISM; RNA, GUIDE; RNA, MESSENGER; ZYGOTE;

EID: 84990068709     PISSN: 00121606     EISSN: 1095564X     Source Type: Journal    
DOI: 10.1016/j.ydbio.2016.07.017     Document Type: Article

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