Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Bioresources and Bioprocessing

Volumn 2, Issue 1, 2015, Pages

  Arora, Richa ^a,b   Behera, Shuvashish ^a   Sharma, Nilesh Kumar ^a,b   Kumar, Sachin ^a  

^a Sardar Swaran Singh National Institute of Bio Energy (Formerly Sardar Swaran Singh National Institute of Renewable Energy)   (India)

^b I K GUJRAL PUNJAB TECHNICAL UNIVERSITY   (India)

Author keywords

Cellulose hydrolysis; Cellulosomes; Nanomachines; Scaffoldin; Synergy

Indexed keywords

BIODEGRADATION; BIOFUELS; BIOMASS; CELLULOSE; COST EFFECTIVENESS; ENZYMES; INDUSTRIAL RESEARCH; MUNICIPAL SOLID WASTE; REFINING; SACCHARIFICATION;

BIOFUEL PRODUCTION; BIOREFINERIES; CELLULOSE HYDROLYSIS; CELLULOSOMES; CLIMATIC CONDITIONS; LIGNOCELLULOSIC BIOMASS; MESOPHILIC; NANOMACHINES; SCAFFOLDIN; SYNERGY;

MICROORGANISMS;

EID: 84982703006     PISSN: None     EISSN: 21974365     Source Type: Journal    
DOI: 10.1186/s40643-015-0066-4     Document Type: Review

References (128)

1. Adams JJ, Webb BA, Spencer HL, Smith SP (2005) Structural characterization of the type II dockerin module from the cellulosome of Clostridium thermocellum: calcium-induced effects on conformation and target recognition. Biochemistry 44:2173–2182
2. Alper H, Stephanopoulos G (2009) Engineering for biofuels: exploiting innate microbial capacity or importing biosynthetic potential? Nat Rev Microbiol 7:715–723
3. Anbar M, Gul O, Lamed R, Sezerman UO, Bayer EA (2012) Improved thermostability of Clostridium thermocellum endoglucanase Cel8A using consensus-guided mutagenesis. Appl Environ Microbiol 287:9213–9221
4. Arora R, Behera S, Sharma NK, Singh R, Yadav YK, Kumar S (2014) Biochemical conversion of rice straw (Oryza sativa L.) to bioethanol using thermotolerant isolate K. marxianus NIRE-K3. In: Sharma NR, Thakur RC, Sharma M, Parihar L, Kumar G (eds) Proceedings of exploring and basic sciences for Next Generation Frontiers. Elsevier, New Delhi, pp 143–146
5. Arora R, Behera S, Kumar S (2015) Bioprospecting thermophilic/thermotolerant microbes for production of lignocellulosic ethanol: A future perspective. Renew Sust Energy Rev 51:699–717
6. Bayer EA, Morag E, Lamed R (1994) The cellulosome: a treasure-trove for biotechnology. Trends Biotechnol 12:379–386
7. Bayer EA, Belaich JP, Shoham Y, Lamed R (2004) The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. Annu Rev Microbiol 58:521–554
8. Bayer EA, Lamed R, Himmel ME (2007) The potential of cellulases and cellulosomes for cellulosic waste management. Curr Opin Biotechnol 18:237–245
9. Bayer EA, Lamed R, White BA, Flint HJ (2008) From cellulosomes to cellulosomics. Chem Rec 8:364–377
10. Bayer EA, Smith SP, Noach I, Alber O, Adams JJ, Lamed R, Shimon LJW, Frolow F (2009) Can we crystallize a cellulosome? In: Karita S, Kimura T, Sakka M, Matsui H, Miyake H, Tanaka A, Sakka K (eds) Biotechnology of lignocellulose degradation and biomass utilization. Ito Print Publishing, Tokyo, pp 183–205
11. Behera S, Arora R, Kumar S (2013) Bioprospecting the cellulases and xylanases thermozymes for the production of biofuels. In: Paper presented at AICHE Annual Meeting, San Francisco, 3–8 November, 2013
12. Behera S, Arora R, Nandhagopal N, Kumar S (2014) Importance of chemical pretreatment for bioconversion of lignocellulosic biomass. Renew Sust Energy Rev 36:91–106
13. Belaich JP, Tardif C, Belaich A, Gaudin C (1997) The cellulolytic system of Clostridium cehlolyticum. J Biotechnol 57:3–14
14. Blanchette C, Lacayo CI, Fischer NO, Hwang M, Thelen MP (2012) Enhanced cellulose degradation using cellulase-nanosphere complexes. PLoS One 7:e42116
15. Blouzard JC, Coutinho PM, Fierobe HP, Henrissat B, Lignon S, Tardif C, Pages S, de Philip P (2010) Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics 10:541–554
16. Bomble YJ, Beckham GT, Matthews JF, Nimlos MR, Himmel ME, Crowley MF (2011) Modeling the self-assembly of the cellulosome enzyme complex. J Biol Chem 286:5614–5623
17. Boraston AB, Bolam DN, Gilbert HJ, Davies GJ (2004) Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J 382:769–781
18. Bras JLA, Carvalhi AL, Viegas A, Najmudin S, Alves VD, Prates JAM, Ferreira LMA, Romao MJ, Gilbert HJ, Fontes CMGA (2012) Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin–dockerin complexes. Methods Enzymol 510:395–415
19. Cadena EM, Chriac AI, Pastor FI, Diaz P, Vidal T, Torres AL (2010) Use of cellulases and recombinant cellulose binding domains for refining TCF kraft pulp. Biotechnol Prog 26:960–967
20. Caspi J, Irwin D, Lamed R, Li Y, Fierobe HP, Wilson DB, Bayer EA (2008) Conversion of Thermobifida fusca free exoglucanases into cellulosomal components: comparative impact on cellulose-degrading activity. J Biotechnol 135:351–357
21. Chanal A, Mingardon F, Bauzan M, Tardif C, Fierobe HP (2011) Scaffoldin modules can serve as ‘‘cargo’’ domains to promote the secretion of heterologous cellulosomal cellulases by Clostridium acetobutylicum. Appl Environ Microbiol 77:6277–6280
22. Chen C, Cui Z, Xiao Y, Cui Q, Smith SP, Lamed R, Bayer EA, Feng Y (2014) Revisiting the NMR solution structure of the Cel48S type-I dockerin module from Clostridium thermocellum reveals a cohesin-primed conformation. J Struct Biol 188:188–193
23. Cho W, Jeon SD, Shim HJ, Doi RH, Han SO (2010) Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker. J Biotechnol 145:233–239
24. Chundawat SPS, Beckham GT, Himmel ME, Dale BE (2011) Deconstruction of lignocellulosic biomass to fuels and chemicals. Annu Rev Chem Biomol Eng 2:121–145
25. Desvaux M (2005) Clostridium cellulolyticum model organism of mesophilic cellulolytic clostridia. FEMS Microbiol Rev 29:741–764
26. Desvaux M, Dumas E, Chafsey I, Hebraud M (2006) Protein cell surface display in Gram-positive bacteria: from single protein to macromolecular protein structure. FEMS Microbiol Lett 256:1–15
27. Desvaux M, Hebraud M, Taylor R, Henderson IR (2009) Secretion and subcellular localizations of bacterial proteins: a semantic awareness issue. Trends Microbiol 17:139–145
28. Ding SY, Xu Q, Crowley M, Zeng Y, Nimlos M, Lamed R, Bayer EA, Himmel ME (2008) A biophysical perspective on the cellulosome: new opportunities for biomass conversion. Curr Opin Biotechnol 19:218–227
29. Ding SY, Liu Y-S, Zeng Y, Himmel ME, Baker JO, Bayer EA (2012) How does plant cell wall nanoscale architecture correlate with enzymatic digestibility? Science 338:1055–1060
30. Doi RH (2008) Cellulases of mesophilic microorganisms: cellulosome and noncellulosome producers. Ann N Y Acad Sci 1125:267–279
31. Doi RH, Kosugi A (2004) Cellulosomes: plant cell wall degrading enzyme complexes. Nat Rev (Microbiol) 2:541–551
32. Elkins JG, Raman B, Keller M (2010) Engineered microbial systems for enhanced conversion of lignocellulosic biomass. Curr Opin Biotechnol 21:657–662
33. Fan LH, Zhang ZJ, Yu XY, Xue YX, Tan TW (2012) Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production. Proc Natl Acad Sci USA 109:13260–13265
34. Fierobe H-P, Mechaly A, Tardif C, Belaich A, Lamed R, Shoham Y, Belaich JP, Bayer EA (2001) Design and production of active cellulosome chimeras: selective incorporation of dockerin-containing enzymes into defined functional complexes. J Biol Chem 276:21257–21261
35. Fierobe HP, Mingardon F, Mechaly A, Belaich A, Rincon MT, Pages S, Lamed R, Tardif C, Belaich JP, Bayer EA (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. J Biol Chem 280:16325–16334
36. Fontes C, Gilbert HJ (2010) Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu Rev Biochem 79:655–681
37. Fukuda T, Isogawa D, Takagi M, Kato-Murai M, Kimoto H, Kusaoke H, Ueda M, Suye S (2007) Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis. Biosci Biotechnol Biochem 71:2845–2847
38. Fukuda T, Tsuchiyama K, Makishima H, Takayama K, Mulchandani A, Kuroda K, Ueda M, Suye S (2010) Improvement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system. Biotechnol Lett 32:655–659
39. Gao SH, You C, Renneckar S, Bao J, Zhang YHP (2014) New insights into enzymatic hydrolysis of heterogeneous cellulose by using carbohydrate-binding module 3 containing GFP and carbohydrate-binding module 17 containing CFP. Biotechnol Biofuels 7:24
40. Garcia-Alvarez B, Melero R, Dias FM, Prates JA, Fontes CM, Smith SP, Romao MJ, Carvalho AL, Llotca O (2011) Molecular architecture and structural transitions of a CLostridium thermocellum mini-cellulosome. J Mol Biol 407:571–580
41. Garvey M, Klose H, Fischer R, Lambertz C, Commandeur U (2013) Cellulases for biomass degradation: comparing recombinant cellulose expression platforms. Trends Biotechnol 31:581–593
42. Gefen G, Anbar M, Morag E, Lamed R, Bayer EA (2012) Enhanced cellulose degradation by targeted integration of a cohesin-fused β-glucosidase into the Clostridium thermocellum cellulosome. Proc Natl Acad Sci USA 109:10298–10303
43. Gourlay K, Arantes V, Saddler JN (2012) Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis. Biotechnol Biofuels 5:51
44. Goyal G, Tsai S-L, Madan B, DaSilva NA, Chen W (2011) Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome. Microb Cell Fact 10:89
45. Hasunuma T, Kondo A (2012) Development of yeast cell factories for consolidated bioprocessing of lignocelluloses to bioethanol through cell surface engineering. Biotechnol Adv 30:1207–1218
46. Hemme CL, Mouttaki H, Lee YJ, Zhang G, Goodwin L, Lucas S, Copeland A, Lapidus A, Glavina del Rio T, Tice H, Saunders E, Brettin T, Detter JC, Han CS, Pitlick S, Land ML, Hauser LJ, Kyrpides N, Mikhailova N, He Z, Wu L, Van Nostrand JD, Henrissat B, He Q, Lawson PA, Tanner RS, Lynd LR, Wiegel J, Fields MW, Arkin AP, Schadt CW, Stevenson BS, Mclnerney MJ, Yang Y, Dong H, Xing D, Ren N, Wang A, Huhnke RL, Mielenz JR, Ding SY, Himmel ME, Taghavi S, van der Lelie D, Rubin EM, Zhou J (2010) Sequencing of multiple clostridial genomes related to biomass conversion and biofuel production. J Bacteriol 192:6494–6496
47. Hendrix J, Fried D, Barak Y, Bayer EA, Lamb DC (2013) Conformational dynamics in designer cellulosomes studied by single-pair fret with Mfd-Pie. Biophys J 104:19a
48. Himmel ME, Xu Q, Luo Y, Ding SY, Lamed R, Bayer EA (2010) Microbial enzyme systems for biomass conversion: emerging paradigms. Biofuels 1:323–341
49. Horn JH, Vaaje-Kolstad G, Westereng B, Eijsink VGH (2012) Novel enzymes for the degradation of cellulose. Biotechnol Biofuels 5:1–12
50. Hussack G, Luo Y, Veldhuis L, Hall JC, Tanha J, Mackenzie R (2009) Multivalent anchoring and oriented display of single-domain antibodies on cellulose. Sensors 9:5351–5367
51. Hyeon JE, Jeon WJ, Whang SY, Han SO (2011) Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum. Enzyme Microb Technol 48:371–377
52. Hyeon JE, Kang DH, Kim YI, Jeon SD, You SK, Kim KY, Kim SW, Han SO (2012) Production of functional agarolytic nano-complex for the synergistic hydrolysis of marine biomass and its potential application in carbohydrate-binding module-utilizing one-step purification. Process Biochem 47:877–881
53. Hyeon JE, Jeon SD, Han SO (2013) Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development: advances and applications. Biotechnol Adv 31:936–944
54. Ichikawa S, Karita S, Kondo M, Goto M (2014) Cellulosomal carbohydrate-binding module from Clostridium josui binds to crystalline and non-crystalline cellulose, and soluble polysaccharides. FEBS Lett 588:3886–3890
55. Inaba C, Higuchi S, Morisaka H, Kuroda K, Ueda M (2010) Synthesis of functional dipeptide carnosine from nonprotected amino acids using carnosinase-displaying yeast cells. Appl Microbiol Biotechnol 86:1895–1902
56. Ito J, Kosugi A, Tanaka T, Kuroda K, Shibasaki S, Ogino C, Ueda M, Fukuda H, Dsoi RH, Kondo A (2009) Regulation of the display ratio of enzymes on the Sacchromyces cerevisiae cell surface by the immunoglobulin G and cellulosomal enzyme binding domains. Appl Environ Microbiol 75:4149–4154
57. Jeon SD, Lee JE, Kim SJ, Kim SW, Han SO (2012) Analysis of selective, high protein–protein binding interaction of cohesin–dockerin complex using biosensing methods. Biosens Bioelectron 35:382–389
58. Ju LK, Afolabi OA (1999) Wastepaper hydrolysate as soluble inducing substrate for cellulase production in continuous culture of Trichoderma reesei. Biotechnol Prog 15:91–97
59. Juturu V, Wu JC (2014) Microbial cellulases: Engineering, production and applications. Renew Sust Energy Rev 33:188–203
60. Karmakar M, Ray RR (2011) Current trends in research and application of microbial cellulases. Res J Microbiol 6:41–53
61. Karpol A, Jobby MK, Slutzki M, Noach I, Chitayat S, Smith SP, Bayer EA (2013) Structural and functional characterization of a novel type-III dockerin from Ruminococcus flavefaciens. FEBS Lett 587:30–36
62. Kaya M, Ito J, Kotaka A, Matsumura K, Bando H, Sahara H, Ogino C, Shibasaki S, Kuroda K, Ueda M, Kondo A, Hata Y (2008) Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface. Appl Microbiol Biotechnol 79:51–60
63. Kim S, Baek S-H, Lee K, Hahn J-S (2013) Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and beta-glucosidase. Microb Cell Fact 12:14
64. Koukiekolo R, Cho HY, Kosugi A, Inui M, Yukawa H, Doi RH (2005) Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA. Appl Environ Microbiol 71:3504–3511
65. Krauss J, Zverlov VV, Schwarz WH (2012) In vitro reconstitution of the complete Clostridium thermocellum cellulosome and synergistic activity on crystalline cellulose. Appl Environ Microbiol 78:4301–4307
66. Kuhad RC, Gupta R, Singh A (2011) Microbial cellulases and their industrial applications. Enzyme Res 2011:1–10
67. Kumar R, Singh S, Singh OV (2008) Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J Ind Microbiol Biotechnol 35:377–391
68. Kumar S, Singh SP, Mishra IM, Adhikari DK (2010) Feasibility of ethanol production with enhanced sugar concentration in bagasse hydrolysate at high temperature using Kluyveromyces sp. IIPE453. Biofuels 1:697–704
69. Kuroda K, Ueda M (2010) Engineering of microorganisms towards recovery of rare metal ions. Appl Microbiol Biotechnol 87:53–60
70. Kuroda K, Ueda M (2011) Molecular design of the microbial cell surface toward the recovery of metal ions. Curr Opin Biotechnol 22:427–433
71. Kuroda K, Nishitani T, Ueda M (2012) Specific adsorption of tungstate by cell surface display of the newly designed ModE mutant. Appl Microbiol Biotechnol 96:153–159
72. Lambertz C, Garvey M, Klinger J, Hessel D, Klose H, Fischer R, Commandeur U (2014) Challenges and advances in the heterologous expression of cellulolytic enzymes: a review. Biotechnol Biofuels 7:135
73. Lamed R, Setter E, Bayer EA (1983) Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum. J Bacteriol 156:828–836
74. Lavan LM, Van Dyk JS, Chan H, Dsoi RH, Pletschke BI (2009) Effect of physical conditions and chemicals on the binding of a mini-CbpA from Clostridium cellulovorans to a semi-crystalline cellulose ligand. Lett Appl Microbiol 48:419–425
75. Li D-C, Li A-N, Papageorgiou AC (2011) Cellulases from thermophilic fungi: recent insights and biotechnological potential. Enzyme Res 2011:308730
76. Liu G, Yue L, Chi Z, Yu W, Chi Z, Madzak C (2009) The surface display of the alginate lyase on the cells of Yarrowia lipolytica for hydrolysis of alginate. Mar Biotechnol (NY) 11:619–626
77. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66:506–577
78. Matano Y, Hasunuma T, Kondo A (2012a) Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulose with a cell surface-engineered yeast strain. Appl Microbiol Biotechnol 97:2231–2237
79. Matano Y, Hasunuma T, Kondo A (2012b) Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass. Bioresour Technol 108:128–133
80. Matsuoka H, Hashimoto K, Saijo A, Takada Y, Kondo A, Ueda M, Ooshima H, Tachibana T, Azuma M (2014) Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae. Yeast 31:67–76
81. Mazzoli R (2012) Development of microorganisms for cellulose-biofuel consolidated bioprocessings: metabolic engineers tricks. Comput Struct Biotechnol J 3:1–9
82. Mazzoli R, Lamberti C, Pessione E (2012) Engineering new metabolic capabilities in bacteria: lessons from recombinant cellulolytic strategies. Trends Biotechnol 30:111–119
83. Mitsuzawa S, Kagawa H, Li Y, Chan SL, Paavola CD, Trent JD (2009) The rosettazyme: a synthetic cellulosome. J Biotechnol 143:139–144
84. Molinier AL, Nouailler M, Valette O, Tardif C, Receveur-Brechot V, Fierobe HP (2011) Synergy, structure and conformational flexibility of hybrid cellulosomes displaying various inter-cohesins linkers. J Mol Biol 405:143–157
85. Morais S, Barak Y, Caspi J, Hadar Y, Lamed R, Shoham Y, Wilson DB, Bayer EA (2010) Cellulase-xylanase synergy in designer cellulosomes for enhanced degradation of a complex cellulosic substrate. MBio 1:e00285–e00310
86. Nakashima K, Yamaguchi K, Taniguchi N, Arai S, Yamada R, Katahira S, Ishida N, Takahashi H, Ogino C, Kondo A (2011) Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment. Green Chem 13:2948–2953
87. Nataf Y, Bahari L, Kahel-Raifer H, Borovok I, Lamed R, Bayer EA, Sonenshein AL, Shoham Y (2010) Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Proc Natl Acad Sci USA 107:18646–18651
88. Nishitani T, Shimada M, Kuroda K, Ueda M (2010) Molecular design of yeast cell surface for adsorption and recovery of molybdenum, one of rare metals. Appl Microbiol Biotechnol 86:641–648
89. Oliveira C, Carvalho V, Domingues L, Gama FM (2015) Recombinant CBM-fusion technology—applications overview. Biotechnol Adv 33:358–369
90. Prasetyo J, Naruse K, Kato T, Boonchird C, Harashima S, Park EY (2011) Bioconversion of paper sludge to biofuel by simultaneous saccharification and fermentation using a cellulase of paper sludge origin and thermotolerant Saccharomyces cerevisiae TJ14. Biotechnol Biofuels 4:35
91. Raman B, Pan C, Hurst GB, Rodriguez JM, McKeown CK, Lankford PK, Samatova NF, Mielenz JR (2009) Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis. PLoS One 4:e5271
92. Ramos R, Domingues L, Gama M (2010) Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum. Protein Expr Purif 71:1–7
93. Ramos R, Moreira S, Rodrigues A, Gama M, Domingues L (2013) Recombinant expression and purification of the antimicrobial peptide magainin-2. Biotechnol Prog 29:17–22
94. Rollin JA, Zhu Z, Sathisuksanoh N, Zhang HP (2011) Increasing cellulose accessibility is more important than removing lignin: a comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia. Biotechnol Bioeng 108:22–30
95. Sadhu S, Maiti TK (2013) Cellulase production by bacteria: a review. British Microbiol Res J 3:235–258
96. Schwarz WH (2001) The cellulosome and cellulose degradation by anaerobic bacteria. Appl Microbiol Biotechnol 56:634–649
97. Shahriarinour M, Wahab MNA, Mohamad R, Mustafa S, Ariff AB (2011) Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus. Afr J Biotechnol 10:7459–7467
98. Shi X, Zheng F, Pan R, Wang J, Ding S (2014) Engineering and comparative characteristics of double carbohydrate binding modules as a strength additive for papermaking applications. Bioresources 9:3117–3131
99. Shoham Y, Lamed R, Bayer EA (1999) The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides. Trends Microbiol 275:275–281
100. Smith SP, Bayer EA (2013) Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex. Curr Opin Struct Biol 23:686–694
101. Stern J, Anbar M, Morais S, Lamed R, Bayer EA (2014) Insights into enhanced thermostability of a cellulosomal enzyme. Carbohyd Res 389:78–84
102. Su GD, Huang DF, Han SY, Zheng SP, Lin Y (2010) Display of Candida antarctica lipase B on Pichia pastoris and its application to flavor ester synthesis. Appl Microbiol Biotechnol 86:1493–1501
103. Sukumaran RK, Singhania RR, Pandey A (2005) Microbial cellulases: production, application and challenges. J Sci Ind Res 64:832–844
104. Suzuki H, Imaedaa T, Kitagawa T, Kohda K (2012) Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae. J Biotechnol 157:64–70
105. Tamaru Y, Karita S, Ibrahim A, Chan H, Dsoi RH (2000) A large gene cluster for the Clostridium cellulovorans cellulosome. J Bacteriol 182:5906–5910
106. Tamaru Y, Miyake H, Kuroda K, Ueda M, Doi RH (2010) Comparative genomics of the mesophilic cellulosome-producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing. Environ Technol 31:889–903
107. Tanaka T, Kondo A (2015) Cell surface engineering of industrial microorganisms for biorefining applications. Biotechnol Adv (in press)
108. Taylor TJ, Vaisman II (2010) Discrimination of thermophilic and mesophilic proteins. BMC Struct Biol 10:S5
109. Tsai SL, Oh J, Singh S, Chen R, Chen W (2009) Functional assembly of minicellulosomes on the Saccharomyces cerevisiae cell surface for cellulose hydrolysis and ethanol production. Appl Environ Microbiol 75:6087–6093
110. Tsai SL, Goyal G, Chen W (2010) Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and ethanol production. Appl Environ Microbiol 76:7514–7520
111. Tsai SL, DaSilva NA, Chen W (2013) Functional display of complex cellulosomes on the yeast surface via adaptive assembly. ACS Synth Biol 2:14–21
112. Vazana Y, Morais S, Barak Y, Lamed R, Bayer EA (2010) Interplay between Clostridium thermocellum family 48 and family 9 cellulases in cellulosomal versus noncellulosomal states. Appl Environ Microbiol 76:3236–3243
113. Vazana Y, Morai S, Barak Y, Lamed R, Bayer EA (2012) Designer cellulosomes for enhanced hydrolysis of cellulosic substrates. Methods Enzymol 510:429–452
114. Vazana Y, Barak Y, Unger T, Peleg Y, Shamshoum M, Ben-Yehezkel T, Mazor Y, Shapiro E, Lamed R, Bayer EA (2013) A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates. Biotechnol Biofuels 6:182
115. Vodovnik M, Logar RM (2010) Cellulosomes-promising supramolecular machines of anaerobic cellulolytic microorganisms. Acta Chim Slov 57:767–774
116. Waeonukul R, Kosugi A, Prawitwong P, Deng L, Tachaapaikoon C, Pason P, Ratanakhanokchai K, Saito M, Mori Y (2013) Novel cellulase recycling method using a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase. Bioresour Technol 130:424–430
117. Wan W, Wang D, Gao X, Hong J (2011) Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast. Appl Microbiol Biotechnol 91:789–798
118. Wen F, Sun J, Zhao H (2010) Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. Appl Environ Microbiol 76:1251–1260
119. Wieczorek AS, Martin VJ (2010) Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis. Microb Cell Fact 9:69
120. Wilson DB (2011) Microbial diversity of cellulose hydrolysis. Curr Opin Microbiol 14:259–263
121. Wu I, Arnold FH (2013) Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures. Biotechnol Bioeng 110:1874–1883
122. Xu Z, Bae W, Mulchandani A, Mehra RK, Chen W (2002) Heavy metal removal by novel CBD-EC20 sorbents immobilized on cellulose. Biomacromolecules 3:462–465
123. Yamada R, Hasunuma T, Kondo A (2013) Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing. Biotechnol Adv 31:754–763
124. Yanase S, Hasunuma T, Yamada R, Tanaka T, Ogino C, Fukuda H, Kondo A (2010a) Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. Appl Microbiol Biotechnol 88:381–388
125. Yanase S, Yamada R, Kaneko S, Noda H, Hasunuma T, Tanaka T, Ogino C, Fukuda H, Kondo A (2010b) Ethanol production from cellulosic materials using cellulase-expressing yeast. Biotechnol J 5:449–455
126. Yaniv O, Jindou S, Frolow F, Lamed R, Bayer EA (2012) A simple method for determining specificity of carbohydrate-binding modules for purified and crude insoluble polysaccharide substrates. Methods Mol Biol 908:101–107
127. You C, Zhang XZ, Sathitsuksanoh N, Lynd LR, Zhang YH (2012a) Enhanced microbial utilization of recalcitrant cellulose by an exvivo cellulosome-microbe complex. Appl Environ Microbiol 78:1437–1444
128. You C, Zhang XZ, Zhang YHP (2012b) Mini-scaffoldin enhanced mini-cellulosome hydrolysis performance on low-accessibility cellulose (Avicel) more than on high-accessibility amorphous cellulose. Biochem Eng J 63:57–65