In vitro reconstitution of the complete Clostridium thermocellum cellulosome and synergistic activity on crystalline cellulose

Applied and Environmental Microbiology

Volumn 78, Issue 12, 2012, Pages 4301-4307

  Krauss, Jan ^a   Zverlov, Vladimir V ^a,b   Schwarz, Wolfgang H ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATE-BINDING MODULES; CELLULASE COMPLEX; CELLULOSIC COMPOUNDS; CELLULOSOMES; CLOSTRIDIUM THERMOCELLUM; COHESINS; CRYSTALLINE CELLULOSE; CRYSTALLINE SUBSTRATES; HYDROLYTIC ACTIVITIES; IN-VITRO; PROTEIN COMPLEXES; SUPERNATANTS; SYNERGISTIC ACTIVITY;

CARBOHYDRATES; CELLULOSE; COMPLEXATION; ENZYMES; PROTEINS;

CRYSTALLINE MATERIALS;

BACTERIAL PROTEIN; CARRIER PROTEIN; CELLULASE; CELLULOSE; CELLULOSOME, CLOSTRIDIUM; MULTIENZYME COMPLEX; RECOMBINANT PROTEIN;

BACTERIUM; CARBOHYDRATE; CELLULOSE; COMPLEXATION; ENZYME ACTIVITY; HYDROLYSIS; PROTEIN; SYNERGISM;

ARTICLE; CHEMISTRY; CLOSTRIDIUM THERMOCELLUM; ENZYMOLOGY; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN MULTIMERIZATION;

BACTERIAL PROTEINS; CARRIER PROTEINS; CELLULASE; CELLULOSE; CLOSTRIDIUM THERMOCELLUM; HYDROLYSIS; MULTIENZYME COMPLEXES; PROTEIN MULTIMERIZATION; RECOMBINANT PROTEINS;

CLOSTRIDIUM THERMOCELLUM;

EID: 84864089016     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07959-11     Document Type: Article

References (45)

1. Bayer EA, Kenig R, Lamed R. 1983. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156:818-827.
2. Bayer EA, Setter E, Lamed R. 1985. Organization and distribution of the cellulosome in Clostridium thermocellum. J. Bacteriol. 163:552-559.
3. Bélaich JP, Tardif C, Bélaich A, Gaudin C. 1997. The cellulolytic system of Clostridium cellulolyticum. J. Biotechnol. 57:3-14.
4. Bomble YJ, et al. 2011. Modeling the self-assembly of the cellulosome enzyme complex. J. Biol. Chem. 286:5614-5623.
5. Caruso F. 2000. Hollow capsule processing through colloidal templating and self-assembly. Chemistry 6:413-419.
6. Caspi J, et al. 2009. Effect of linker length and dockerin position on conversion of a Thermobifida fusca endoglucanase to the cellulosomal mode. Appl. Environ. Microbiol. 75:7335-7342.
7. Cho HY, Yukawa H, Inui M, Doi RH, Wong SL. 2004. Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Appl. Environ. Microbiol. 70:5704-5707.
8. Coughlan MP, et al. 1985. The cellulolytic enzyme complex of Clostridium thermocellum is very large. Biochem. Biophys. Res. Commun. 130: 904-909.
9. Fierobe HP, et al. 1999. Cellulosome from Clostridium cellulolyticum: molecular study of the dockerin/cohesin interaction. Biochemistry 38: 12822-12832.
10. Fierobe HP, et al. 2002. Degradation of cellulose substrates by cellulosome chimeras. J. Biol. Chem. 277:49621-49630.
11. Fierobe HP, et al. 2005. Action of designer cellulosomes on homogeneous versus complex substrates: Controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. J. Biol. Chem. 280:16325-16334.
12. Gold ND, Martin VJJ. 2007. Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. J. Bacteriol. 189: 6787-6795.
13. Jindou S, et al. 2004. Cohesin-dockerin interactions within and between Clostridium josui and Clostridium thermocellum: binding selectivity between cognate dockerin and cohesin domains and species specificity. J. Biol. Chem. 279:9867-9874.
14. Johnson EA, Madia A, Demain AL. 1981. Chemically defined minimal medium for growth of the anaerobic cellulolytic thermophile Clostridium thermocellum. Appl. Environ. Microbiol. 41:1060-1062.
15. Kim JS, Valencia CA, Liu R, Lin W. 2007. Highly-efficient purification of native polyhistidine-tagged proteins by multivalent NTA-modified magnetic nanoparticles. Bioconjug. Chem. 18:333-341.
16. Kouassi GK, Irudayaraj J, McCarty G. 2005. Examination of cholesterol oxidase attachment onto magnetic nanoparticles. J. Nanobiotechnol. 3:1.
17. Lamed R, Setter E, Bayer EA. 1983. Characterization of a cellulosebinding, cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. 156:828-836.
18. Lauer SA, Nolan JP. 2002. Development and characterization of Ni-NTA-bearing microspheres. Cytometry 48:136-145.
19. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66:506-577.
20. Lytle B, Myers C, Kruus K, Wu JH. 1996. Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA. J. Bacteriol. 178:1200-1203.
21. Mayer F, Coughlan MP, Mori Y, Ljungdahl LG. 1987. Macromolecular organization of the cellulolytic enzyme complex of Clostridium thermocellum as revealed by electron microscopy. Appl. Environ. Microbiol. 53: 2785-2792.
22. Mechaly A, et al. 2000. Cohesin-dockerin recognition in cellulosome assembly: experiment versus hypothesis. Proteins 39:170-177.
23. Mechaly A, et al. 2001. Cohesin-dockerin interaction in cellulosome assembly: a single hydroxyl group of a dockerin domain distinguishes between nonrecognition and high affinity recognition. J. Biol. Chem. 13: 9883-9888.
24. Merino ST, Cherry J. 2007. Progress and challenges in enzyme development for biomass utilization. Adv. Biochem. Eng. Biotechnol. 108:95-120.
25. Mitsuzawa S, et al. 2009. The rosettazyme: a synthetic cellulosome. J. Biotechnol. 143:139-144.
26. Morag E, et al. 1996. Dissociation of the cellulosome of Clostridium thermocellum under nondenaturing conditions. J. Biotechnol. 51:235-242.
27. Morag E, Bayer EA, Lamed R. 1992. Affinity digestion for the near-total recovery of purified cellulosome from Clostridium thermocellum. Enzyme Microb. Technol. 14:289-292.
28. Moraïs S, et al. 2011. Assembly of xylanases into designer cellulosomes promotes efficient hydrolysis of the xylan component of a natural recalcitrant cellulosic substrate. mBio 2(6):e00233-11. doi:10.1128/ mBio.00233-11.
29. Raman B, et al. 2009. Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis. PLoS One 4:e5271. doi: 10.1371/journal.pone.0005271.
30. Raman B, McKeown CK, Rodriguez M, Brown SD, Mielenz JR. 2011. Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation. BMC Microbiol. 11:134.
31. Schwarz WH. 2001. The cellulosome and cellulose degradation by anaerobic bacteria. Appl. Microbiol. Biotechnol. 56:634-649.
32. Schwarz WH, Zverlov VV, Bahl H. 2004. Extracellular glycosyl hydrolases from clostridia. Adv. Appl. Microbiol. 56:215-261.
33. Staros JV, Wright RW, Swingle DM. 1986. Enhancement by Nhydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reaction. Anal. Biochem. 156:220-222.
34. Tsai SL, Oh J, Singh S, Chen R, Chen W. 2009. Functional assembly of minicellulosomes on the Saccharomyces cerevisiae cell surface for cellulose hydrolysis and ethanol production. Appl. Environ. Microbiol. 75:6087-6093.
35. Wang WK, Kruus K, Wu JHD. 1994. Cloning and expression of the Clostridium thermocellum celS gene in Escherichia coli. Appl. Microbiol. Biotechnol. 42:346-352.
36. Weimer PJ, Zeikus JG. 1977. Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence of Methanobacterium thermoautotrophicum. Appl. Environ. Microbiol. 33:289-297.
37. Wen F, Sun J, Zhao H. 2010. Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. Appl. Environ. Microbiol. 76:1251-1260.
38. Wood TM, Bhat KM. 1988. Methods for measuring cellulase activities. Methods Enzymol. 160:87-112.
39. Yaron S, Morag E, Bayer EA, Lamed R, Shoham Y. 1995. Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome. FEBS Let. 360:121-124.
40. Zhang Y, Lynd LR. 2003. Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch cultures. Anal. Chem. 75:219-227.
41. Zhang YHP. 2011. What is vital (and not vital) to advance economicallycompetitive biofuels production. Proc. Biochem. 46:2091-2110.
42. Zverlov VV, Kellermann J, Schwarz WH. 2005. Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes. Proteomics 5:3646-3653.
43. Zverlov VV, Schwarz WH. 2006. The C. thermocellum cellulosome: novel components and insights from the genomic sequence, p 119-151. In Uversky V, Kataeva I (ed), Cellulosome VI. Nova Science, Hauppauge, NY.
44. Zverlov VV, Klupp M, Krauss J, Schwarz WH. 2008. Mutants in the scaffoldin gene cipA of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new IS element, IS1447, and implications for cellulase synergism on crystalline cellulose. J. Bacteriol. 190:4321-4327.
45. Zverlov VV, et al. 2010. Hydrolytic bacteria in mesophilic and thermophilic degradation of plant biomass. Eng. Life Sci. 10:528-536.