-
1
-
-
84858414020
-
Cellular metabolism and disease: What do metabolic outliers teach us?
-
DeBerardinis, R. J. & Thompson, C. B. Cellular metabolism and disease: what do metabolic outliers teach us? Cell 148, 1132-1144 (2012).
-
(2012)
Cell
, vol.148
, pp. 1132-1144
-
-
DeBerardinis, R.J.1
Thompson, C.B.2
-
2
-
-
77952883945
-
Systems biology of microbial metabolism
-
Heinemann M. & Sauer, U. Systems biology of microbial metabolism. Curr. Opin. Microbiol. 13., 337-343 (2010).
-
(2010)
Curr. Opin. Microbiol.
, vol.13
, pp. 337-343
-
-
Heinemann, M.1
Sauer, U.2
-
3
-
-
84872110559
-
Quantitative imaging with fluorescent biosensors
-
Okumoto, S., Jones, A. & Frommer, W. B. Quantitative imaging with fluorescent biosensors. Annu. Rev. Plant Biol. 63, 663-706 (2012).
-
(2012)
Annu. Rev. Plant Biol.
, vol.63
, pp. 663-706
-
-
Okumoto, S.1
Jones, A.2
Frommer, W.B.3
-
4
-
-
2442612916
-
Minimally invasive dynamic imaging of ions and metabolites in living cells
-
Fehr, M., Ehrhardt, D. W., Lalonde, S. & Frommer, W. B. Minimally invasive dynamic imaging of ions and metabolites in living cells. Curr. Opin. Plant Biol. 7, 345-351 (2004).
-
(2004)
Curr. Opin. Plant Biol.
, vol.7
, pp. 345-351
-
-
Fehr, M.1
Ehrhardt, D.W.2
Lalonde, S.3
Frommer, W.B.4
-
5
-
-
70349331596
-
Genetically encoded biosensors based on engineered fluorescent proteins
-
Frommer, W. B., Davidson, M. W. & Campbell, R. E. Genetically encoded biosensors based on engineered fluorescent proteins. Chem. Soc. Rev. 38, 2833 (2009).
-
(2009)
Chem. Soc. Rev.
, vol.38
, pp. 2833
-
-
Frommer, W.B.1
Davidson, M.W.2
Campbell, R.E.3
-
6
-
-
75149112222
-
Designs and applications of fluorescent protein-based biosensors
-
Ibraheem, A. & Campbell, R. E. Designs and applications of fluorescent protein-based biosensors. Curr. Opin. Chem. Biol. 14, 30-36 (2010).
-
(2010)
Curr. Opin. Chem. Biol.
, vol.14
, pp. 30-36
-
-
Ibraheem, A.1
Campbell, R.E.2
-
7
-
-
0035129282
-
A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein
-
Nakai, J., Ohkura, M. & Imoto, K. A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol. 19, 137-141 (2001).
-
(2001)
Nat. Biotechnol.
, Issue.19
, pp. 137-141
-
-
Nakai, J.1
Ohkura, M.2
Imoto, K.3
-
8
-
-
0346500466
-
Creation of an allosteric enzyme by domain insertion
-
Guntas, G. & Ostermeier, M. Creation of an allosteric enzyme by domain insertion. J. Mol. Biol. 336, 263-273 (2004).
-
(2004)
J. Mol. Biol.
, vol.336
, pp. 263-273
-
-
Guntas, G.1
Ostermeier, M.2
-
9
-
-
1942425613
-
Domain insertions in protein structures
-
Aroul-Selvam, R., Hubbard, T. & Sasidharan, R. Domain insertions in protein structures. J. Mol. Biol. 338, 633-641 (2004).
-
(2004)
J. Mol. Biol.
, vol.338
, pp. 633-641
-
-
Aroul-Selvam, R.1
Hubbard, T.2
Sasidharan, R.3
-
10
-
-
77956338533
-
High-resolution mapping of protein sequence-function relationships
-
Fowler, D. M. et al. High-resolution mapping of protein sequence-function relationships. Nat. Methods 7, 741-746 (2010).
-
(2010)
Nat. Methods
, vol.7
, pp. 741-746
-
-
Fowler, D.M.1
-
11
-
-
84862025262
-
Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing
-
Whitehead, T. A. et al. Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing. Nat. Biotechnol. 30, 543-548 (2012).
-
(2012)
Nat. Biotechnol.
, vol.30
, pp. 543-548
-
-
Whitehead, T.A.1
-
12
-
-
37349052779
-
Functional fusion proteins by random transposon-based GFP insertion
-
Mealer, R., Butler, H. & Hughes, T. Functional fusion proteins by random transposon-based GFP insertion. Methods Cell Biol. 85, 23-44 (2008).
-
(2008)
Methods Cell Biol.
, vol.85
, pp. 23-44
-
-
Mealer, R.1
Butler, H.2
Hughes, T.3
-
13
-
-
48349086462
-
Linking the functions of unrelated proteins using a novel directed evolution domain insertion method
-
Edwards, W. R., Busse, K., Allemann, R. K. & Jones, D. D. Linking the functions of unrelated proteins using a novel directed evolution domain insertion method. Nucleic Acids Res. 36, e78-e78 (2008).
-
(2008)
Nucleic Acids Res.
, vol.36
, pp. e78-e78
-
-
Edwards, W.R.1
Busse, K.2
Allemann, R.K.3
Jones, D.D.4
-
14
-
-
0031571605
-
Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor
-
Quiocho, F. A., Spurlino, J. C. & Rodseth, L. E. Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor. Structure 5, 997-1015 (1997).
-
(1997)
Structure
, vol.5
, pp. 997-1015
-
-
Quiocho, F.A.1
Spurlino, J.C.2
Rodseth, L.E.3
-
15
-
-
0035951304
-
The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85A
-
Diez, J. et al. The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85A. J. Mol. Biol. 305, 905-915 (2001).
-
(2001)
J. Mol. Biol.
, vol.305
, pp. 905-915
-
-
Diez, J.1
-
16
-
-
0029096898
-
The phage Mu transpososome core: DNA requirements for assembly and function
-
Savilahti, H., Rice, P. A. & Mizuuchi, K. The phage Mu transpososome core: DNA requirements for assembly and function. EMBO J. 14, 4893-4903 (1995).
-
(1995)
EMBO J.
, vol.14
, pp. 4893-4903
-
-
Savilahti, H.1
Rice, P.A.2
Mizuuchi, K.3
-
17
-
-
0032897710
-
An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction
-
Haapa, S. et al. An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction. Genome Res. 9, 308-315 (1999).
-
(1999)
Genome Res.
, vol.9
, pp. 308-315
-
-
Haapa, S.1
-
18
-
-
56649114274
-
A one pot, one step, precision cloning method with high throughput capability
-
Engler, C., Kandzia, R. & Marillonnet, S. A one pot, one step, precision cloning method with high throughput capability. PLoS ONE 3, e3647 (2008).
-
(2008)
PLoS ONE
, vol.3
, pp. e3647
-
-
Engler, C.1
Kandzia, R.2
Marillonnet, S.3
-
19
-
-
80053936867
-
A genetically encoded, high-signal-to-noise maltose sensor
-
Marvin, J. S., Schreiter, E. R., Echevarria, I. M. & Looger, L. L. A genetically encoded, high-signal-to-noise maltose sensor. Proteins 79, 3025-3036 (2011).
-
(2011)
Proteins
, vol.79
, pp. 3025-3036
-
-
Marvin, J.S.1
Schreiter, E.R.2
Echevarria, I.M.3
Looger, L.L.4
-
20
-
-
0026493924
-
Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxis
-
Sharff, A. J., Rodseth, L. E., Spurlino, J. C. & Quiocho, F. A. Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxis. Biochemistry 31, 10657-10663 (1992).
-
(1992)
Biochemistry
, vol.31
, pp. 10657-10663
-
-
Sharff, A.J.1
Rodseth, L.E.2
Spurlino, J.C.3
Quiocho, F.A.4
-
21
-
-
0035875870
-
Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy
-
Evenas, J. et al. Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy. J. Mol. Biol. 309, 961-974 (2001).
-
(2001)
J. Mol. Biol.
, vol.309
, pp. 961-974
-
-
Evenas, J.1
-
22
-
-
65249106110
-
Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design
-
Akerboom, J. et al. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem. 284, 6455-6464 (2009).
-
(2009)
J. Biol. Chem.
, vol.284
, pp. 6455-6464
-
-
Akerboom, J.1
-
23
-
-
44949134981
-
Trehalose metabolism and signaling
-
Paul, M. J., Primavesi, L. F., Jhurreea, D. & Zhang, Y. Trehalose metabolism and signaling. Annu. Rev. Plant Biol. 59, 417-441 (2008).
-
(2008)
Annu. Rev. Plant Biol.
, vol.59
, pp. 417-441
-
-
Paul, M.J.1
Primavesi, L.F.2
Jhurreea, D.3
Zhang, Y.4
-
24
-
-
0038056150
-
New insights on trehalose: A multifunctional molecule
-
Elbein, A. D., Pan, Y. T., Pastuszak, I. & Carroll, D. New insights on trehalose: a multifunctional molecule. Glycobiology 13, 17R-27R (2003).
-
(2003)
Glycobiology
, vol.13
, pp. 17R-27R
-
-
Elbein, A.D.1
Pan, Y.T.2
Pastuszak, I.3
Carroll, D.4
-
25
-
-
84958952946
-
FRET sensorbased quantification of intracellular trehalose in mammalian cells
-
Kikuta, S., Hou, B.-H., Sato, R., Frommer, W. B. & Kikawada, T. FRET sensorbased quantification of intracellular trehalose in mammalian cells. Biosci. Biotechnol. Biochem. 80, 162-165 (2015).
-
(2015)
Biosci. Biotechnol. Biochem.
, vol.80
, pp. 162-165
-
-
Kikuta, S.1
Hou, B.-H.2
Sato, R.3
Frommer, W.B.4
Kikawada, T.5
-
26
-
-
84959519456
-
Enzymatic protein switches built from paralogous input domains
-
Tullman, J., Nicholes, N., Dumont, M. R., Ribeiro, L. F. & Ostermeier, M. Enzymatic protein switches built from paralogous input domains. Biotechnol. Bioeng. 113, 852-858 (2015).
-
(2015)
Biotechnol. Bioeng.
, vol.113
, pp. 852-858
-
-
Tullman, J.1
Nicholes, N.2
Dumont, M.R.3
Ribeiro, L.F.4
Ostermeier, M.5
-
27
-
-
0026330981
-
Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli
-
Hengge-Aronis, R., Klein, W., Lange, R., Rimmele, M. & Boos, W. Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli. J. Bacteriol. 173, 7918-7924 (1991).
-
(1991)
J. Bacteriol.
, vol.173
, pp. 7918-7924
-
-
Hengge-Aronis, R.1
Klein, W.2
Lange, R.3
Rimmele, M.4
Boos, W.5
-
28
-
-
84856789240
-
Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity
-
Li, H. et al. Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity. J. Biosci. Bioeng. 113, 224-232 (2012).
-
(2012)
J. Biosci. Bioeng.
, vol.113
, pp. 224-232
-
-
Li, H.1
-
29
-
-
0031905435
-
Trehalose is not relevant for in vivo activity of sigmaS-containing RNA polymerase in Escherichia coli
-
Germer, J., Muffler, A. & Hengge-Aronis, R. Trehalose is not relevant for in vivo activity of sigmaS-containing RNA polymerase in Escherichia coli. J. Bacteriol. 180, 1603-1606 (1998).
-
(1998)
J. Bacteriol.
, vol.180
, pp. 1603-1606
-
-
Germer, J.1
Muffler, A.2
Hengge-Aronis, R.3
-
30
-
-
80051794613
-
Deep mutational scanning: Assessing protein function on a massive scale
-
Araya, C. L. & Fowler, D. M. Deep mutational scanning: assessing protein function on a massive scale. Trends Biotechnol. 29, 435-442 (2011).
-
(2011)
Trends Biotechnol.
, vol.29
, pp. 435-442
-
-
Araya, C.L.1
Fowler, D.M.2
-
31
-
-
84867026349
-
Optimization of a GCaMP calcium indicator for neural activity imaging
-
Akerboom, J. et al. Optimization of a GCaMP calcium indicator for neural activity imaging. J. Neurosci. 32, 13819-13840 (2012).
-
(2012)
J. Neurosci.
, vol.32
, pp. 13819-13840
-
-
Akerboom, J.1
-
32
-
-
84891782659
-
Pfam: The protein families database
-
Finn, R. D. et al. Pfam: the protein families database. Nucleic Acids Res. 42, D222-D230 (2014).
-
(2014)
Nucleic Acids Res.
, vol.42
, pp. D222-D230
-
-
Finn, R.D.1
-
33
-
-
4143115810
-
Periplasmic binding proteins: A versatile superfamily for protein engineering
-
Dwyer, M. A. & Hellinga, H. W. Periplasmic binding proteins: a versatile superfamily for protein engineering. Curr. Opin. Struct. Biol. 14, 495-504 (2004).
-
(2004)
Curr. Opin. Struct. Biol.
, vol.14
, pp. 495-504
-
-
Dwyer, M.A.1
Hellinga, H.W.2
-
34
-
-
84884166196
-
Computational design of ligand-binding proteins with high affinity and selectivity
-
Tinberg, C. E. et al. Computational design of ligand-binding proteins with high affinity and selectivity. Nature 501, 212-216 (2013).
-
(2013)
Nature
, vol.501
, pp. 212-216
-
-
Tinberg, C.E.1
-
35
-
-
48249141040
-
Allostery and cooperativity revisited
-
Cui, Q. & Karplus, M. Allostery and cooperativity revisited. Protein Sci. 17, 1295-1307 (2008).
-
(2008)
Protein Sci.
, vol.17
, pp. 1295-1307
-
-
Cui, Q.1
Karplus, M.2
-
36
-
-
78651189765
-
On the nature of allosteric transitions: A plausible model
-
Monod, J., Wyman, J. & Changeux, J. P. On the nature of allosteric transitions: a plausible model. J. Mol. Biol. 12, 88-118 (1965).
-
(1965)
J. Mol. Biol.
, vol.12
, pp. 88-118
-
-
Monod, J.1
Wyman, J.2
Changeux, J.P.3
-
37
-
-
0013863816
-
Comparison of experimental binding data and theoretical models in proteins containing subunits
-
Koshland, D. E., Nemethy, G. & Filmer, D. Comparison of experimental binding data and theoretical models in proteins containing subunits. Biochemistry 5, 365-385 (1966).
-
(1966)
Biochemistry
, vol.5
, pp. 365-385
-
-
Koshland, D.E.1
Nemethy, G.2
Filmer, D.3
-
39
-
-
84898993517
-
The ensemble nature of allostery
-
Motlagh, H. N., Wrabl, J. O., Li, J. & Hilser, V. J. The ensemble nature of allostery. Nature 508, 331-339 (2014).
-
(2014)
Nature
, vol.508
, pp. 331-339
-
-
Motlagh, H.N.1
Wrabl, J.O.2
Li, J.3
Hilser, V.J.4
-
40
-
-
6344219895
-
Is allostery an intrinsic property of all dynamic proteins?
-
Gunasekaran, K., Ma, B. & Nussinov, R. Is allostery an intrinsic property of all dynamic proteins? Proteins 57, 433-443 (2004).
-
(2004)
Proteins
, vol.57
, pp. 433-443
-
-
Gunasekaran, K.1
Ma, B.2
Nussinov, R.3
-
41
-
-
54249105941
-
Surface sites for engineering allosteric control in proteins
-
Lee, J. et al. Surface sites for engineering allosteric control in proteins. Science 322, 438-442 (2008).
-
(2008)
Science
, vol.322
, pp. 438-442
-
-
Lee, J.1
-
42
-
-
84974602586
-
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
-
Oakes, B. L. et al. Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat. Biotechnol. 34, 646-651 (2016).
-
(2016)
Nat. Biotechnol.
, vol.34
, pp. 646-651
-
-
Oakes, B.L.1
-
43
-
-
84925437370
-
Protein engineering of Cas9 for enhanced function
-
Oakes, B. L., Nadler, D. C. & Savage, D. F. Protein engineering of Cas9 for enhanced function. Methods Enzymol. 546, 491-511 (2014).
-
(2014)
Methods Enzymol.
, vol.546
, pp. 491-511
-
-
Oakes, B.L.1
Nadler, D.C.2
Savage, D.F.3
-
44
-
-
0031663369
-
The green fluorescent protein
-
Tsien, R. Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544 (1998).
-
(1998)
Annu. Rev. Biochem.
, vol.67
, pp. 509-544
-
-
Tsien, R.Y.1
-
45
-
-
0346850576
-
MuA transposase separates DNA sequence recognition from catalysis
-
Goldhaber-Gordon, I., Early, M. H. & Baker, T. A. MuA transposase separates DNA sequence recognition from catalysis. Biochemistry 42, 14633-14642 (2003).
-
(2003)
Biochemistry
, vol.42
, pp. 14633-14642
-
-
Goldhaber-Gordon, I.1
Early, M.H.2
Baker, T.A.3
-
46
-
-
14844294424
-
Protein production by auto-induction in high density shaking cultures
-
Studier, F. W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41, 207-234 (2005).
-
(2005)
Protein Expr. Purif.
, vol.41
, pp. 207-234
-
-
Studier, F.W.1
-
47
-
-
77958471357
-
Differential expression analysis for sequence count data
-
Anders, S. & Huber, W. Differential expression analysis for sequence count data. Genome Biol. 11, R106 (2010).
-
(2010)
Genome Biol.
, vol.11
, pp. R106
-
-
Anders, S.1
Huber, W.2
|