Enzymatic protein switches built from paralogous input domains

Biotechnology and Bioengineering

Volumn 113, Issue 4, 2016, Pages 852-858

  Tullman, Jennifer ^a   Nicholes, Nathan ^a   Dumont, Matt R ^a   Ribeiro, Lucas F ^a   Ostermeier, Marc ^a  

^a Johns Hopkins University   (United States)

Author keywords

Biosensors; Periplasmic binding proteins; Protein engineering; Protein switches; Synthetic biology

Indexed keywords

BINS; BIOLOGY; BIOSENSORS;

BINDING PROTEINS; CIRCULAR PERMUTATION; DIRECTED EVOLUTION; INPUT AND OUTPUTS; PROTEIN ENGINEERING; PROTEIN SWITCHES; RIBOSE-BINDING PROTEINS; SYNTHETIC BIOLOGY;

PROTEINS;

BETA LACTAMASE TEM 1; BINDING PROTEIN; GLUCOSE BINDING PROTEIN; PERIPLASMIC BINDING PROTEIN; PROTEIN; PROTEIN SWITCHES; RIBOSE BINDING PROTEIN; UNCLASSIFIED DRUG; XYLOSE BINDING PROTEIN; BETA LACTAMASE; BETA-LACTAMASE TEM-1; HYBRID PROTEIN;

AMINO ACID COMPOSITION; ANION EXCHANGE; ARTICLE; CALCIUM DEPLETION; HYDROLYSIS; LIGAND BINDING; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; THERMOSTABILITY; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; PROCEDURES; PROTEIN ENGINEERING;

BETA-LACTAMASES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; PERIPLASMIC BINDING PROTEINS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

EID: 84959519456     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25852     Document Type: Article

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