FRET sensor-based quantification of intracellular trehalose in mammalian cells

Bioscience, Biotechnology and Biochemistry

Volumn 80, Issue 1, 2016, Pages 162-165

  Kikuta, Shingo ^a,b   Hou, Bi Huei ^c   Sato, Ryoichi ^b   Frommer, Wolf B ^c   Kikawada, Takahiro ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^c GEOPHYSICAL LABORATORY   (United States)

Author keywords

Biosensor; FRET; Transporter; Trehalose; Visual quantification

Indexed keywords

BIOLOGY; BIOSENSORS; CHEMISTRY;

FRET; MICROMOLAR LEVEL; MOLECULAR MECHANISM; MONITORING TECHNOLOGIES; STRESS PROTECTANTS; TRANSPORTER; TREHALOSE; VISUAL QUANTIFICATION;

MAMMALS;

BACTERIAL PROTEIN; CARRIER PROTEIN; INSECT PROTEIN; PHOTOPROTEIN; TREHALOSE; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL; CHEMISTRY; CYTOPLASM; DIPTERA; DRUG EFFECTS; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; GENE VECTOR; GENETIC PROCEDURES; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; HUMAN; METABOLISM; PROCEDURES;

ANIMALS; BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; CARRIER PROTEINS; CYTOPLASM; DIPTERA; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; GENETIC VECTORS; HEK293 CELLS; HUMANS; INSECT PROTEINS; LUMINESCENT PROTEINS; TRANSFECTION; TREHALOSE;

EID: 84958952946     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1080/09168451.2015.1069699     Document Type: Article

References (21)

1. Argüelles JC. Physiological roles of trehalose in bacteria and yeasts: a comparative analysis. Arch. Microbiol. 2000;174:217-224.
2. Wyatt G. The biochemistry of insect hemolymph. Annu. Rev. Entomol. 1961;6:75-102.
3. Crowe JH, Hoekstra FA, Crowe LM. Anhydrobiosis. Annu. Rev. Physiol. 1992;54:579-599.
4. Sarkar S, Davies JE, Huang Z, Tunnacliffe A, Rubinsztein DC. Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and α-Synuclein. J. Biol. Chem. 2007;282:5641-5652.
5. Sakurai M, Furuki T, Akao K, et al. Vitrification is essential for anhydrobiosis in an African chironomid, Polypedilum vanderplanki. Proc. Natl. Acad. Sci. 2008;105:5093-5098.
6. Chakraborty N, Menze MA, Elmoazzen H, et al. Trehalose transporter from African chironomid larvae improves desiccation tolerance of Chinese hamster ovary cells. Cryobiology. 2012;64:91-96.
7. Li S, Chakraborty N, Borcar A, Menze MA, Toner M, Hand SC. Late embryogenesis abundant proteins protect human hepatoma cells during acute desiccation. Proc. Natl. Acad. Sci. 2012;109:20859-20864.
8. Seki T, Abe-Seki N, Kikawada T, et al. The effect of trehalose on the properties of mutant γPKC, which causes spinocerebellar ataxia type 14 (SCA14), in neuronal cell lines and cultured Purkinje cells. J. Biol. Chem. 2010;285:33252-33264.
9. Kikawada T, Saito A, Kanamori Y, et al. Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells. Proc. Natl. Acad. Sci. 2007;104:11585-11590.
10. Kanamori Y, Saito A, Hagiwara-Komoda Y, et al. The trehalose transporter 1 gene sequence is conserved in insects and encodes proteins with different kinetic properties involved in trehalose import into peripheral tissues. Insect Biochem. Mol. Biol. 2010;40:30-37.
11. San Martín A, Ceballo S, Ruminot I, Lerchundi R, Frommer WB, Barros LF. A genetically encoded FRET lactate sensor and its use to detect the Warburg effect in single cancer cells. PLoS ONE. 2013;8:e57712.
12. Okumoto S, Looger LL, Micheva KD, Reimer RJ, Smith SJ, Frommer WB. Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Proc. Natl. Acad. Sci. 2005;102:8740-8745.
13. Miyawaki A, Llopis J, Heim R, et al. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature. 1997;388:882-887.
14. Heim R, Tsien RY. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 1996;6:178-182.
15. Fehr M, Frommer WB, Lalonde S. Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Proc. Natl. Acad. Sci. 2002;99:9846-9851.
16. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 2002;20:87-90.
17. Lager I, Looger LL, Hilpert M, Lalonde S, Frommer WB. Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose. J. Biol. Chem. 2006;281:30875-30883.
18. Chen L-Q, Qu X-Q, Hou B-H, et al. Sucrose efflux mediated by SWEET proteins as a key step for phloem transport. Science. 2012;335:207-211.
19. Hou B-H, Takanaga H, Grossmann G, et al. Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells. Nat. Protocols. 2011;6:1818-1833.
20. Robinson PS, Goochee CF. Kidney-specific enzyme expression by human kidney cell lines generated through oncogene transfection. J. Cell Physiol. 1991;148:54-59.
21. Nishizaki Y, Yoshizane C, Toshimori Y, et al. Disaccharidetrehalose inhibits bone resorption in ovariectomized mice. Nutr. Res. 2000;20:653-664.