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Where do all the ions go? the cellular basis of differential ion accumulation in leaf cells
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Rubisco small subunit, chlorophyll a/b-binding protein and sucrose:fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light
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A beautiful analysis of sugar signaling in single cells that correlates sugar levels with gene expression. This work demonstrates that although sugar levels measured in whole organs correlate with gene expression in some cases, this does not hold true when these analyses are performed at the single-cell level.
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Lu C., Koroleva O.A., Farrar J.F., Gallagher J., Pollock C.J., Tomos A.D. Rubisco small subunit, chlorophyll a/b-binding protein and sucrose:fructan-6- fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. Cell type specificity and induction by light. Plant Physiol. 130:2002;1335-1348 A beautiful analysis of sugar signaling in single cells that correlates sugar levels with gene expression. This work demonstrates that although sugar levels measured in whole organs correlate with gene expression in some cases, this does not hold true when these analyses are performed at the single-cell level.
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Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a non-aqueous fractionation method
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Farré E.M., Tiessen A., Roessner U., Geigenberger P., Trethewey R.N., Willmitzer L. Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a non-aqueous fractionation method. Plant Physiol. 127:2001;685-700
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Myers R. The biological application of small animal PET imaging. Nucl Med Biol. 28:2001;585-593
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Fluorescence imaging of physiological activity in complex systems using GFP-based probes
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This review provides an up-to-date overview of FRET-based nanosensors for various in vivo applications and the technology required for the measurements. Miyawaki also discusses the potential problems associated with the use of pH-sensitive fluorescent proteins as reporters.
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Miyawaki A. Fluorescence imaging of physiological activity in complex systems using GFP-based probes. Curr Opin Neurobiol. 13:2003;591-596 This review provides an up-to-date overview of FRET-based nanosensors for various in vivo applications and the technology required for the measurements. Miyawaki also discusses the potential problems associated with the use of pH-sensitive fluorescent proteins as reporters.
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Curr Opin Neurobiol
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Gonzalez J.E., Tsien R.Y. Improved indicators of cell membrane potential that use fluorescence resonance energy transfer. Chem Biol. 4:1997;269-277
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Gonzalez J.E., Maher M.P. Cellular fluorescent indicators and voltage/ion probe reader (VIPR) tools for ion channel and receptor drug discovery. Recept Channels. 8:2002;283-295
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Tuning FlaSh: Redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential
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The authors describe the development of a protein-based voltage dye.
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Guerrero G., Siegel M.S., Roska B., Loots E., Isacoff E.Y. Tuning FlaSh: redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential. Biophys J. 83:2002;3607-3618 The authors describe the development of a protein-based voltage dye.
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An excellent review of the development of FRET-based sensors, including the cameleon, for a variety of cellular events.
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Zhang J., Campbell R.E., Ting A.Y., Tsien R.Y. Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol. 3:2002;906-918 An excellent review of the development of FRET-based sensors, including the cameleon, for a variety of cellular events.
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Visualization of maltose uptake in living yeast cells by fluorescent nanosensors
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A prototype for a FRET-based nanosensor is developed on the basis of the periplasmic MBP. Maltose uptake into yeast cells is observed directly by FRET imaging.
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Fehr M., Frommer W.B., Lalonde S. Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Proc Natl Acad Sci USA. 99:2002;9846-9851 A prototype for a FRET-based nanosensor is developed on the basis of the periplasmic MBP. Maltose uptake into yeast cells is observed directly by FRET imaging.
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The authors describe the development of FRET-based nanosensors that are based on bacterial periplasmic glucose-binding proteins and their application in determining glucose concentrations in living animal cells. The sensors identify free glucose in the cytosol and provide evidence that cytosolic glucose does not efflux from COS-7 cells.
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Fehr M., Lalonde S., Lager I., Wolff M.W., Frommer W.B. In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. J Biol Chem. 278:2003;19127-19133 The authors describe the development of FRET-based nanosensors that are based on bacterial periplasmic glucose-binding proteins and their application in determining glucose concentrations in living animal cells. The sensors identify free glucose in the cytosol and provide evidence that cytosolic glucose does not efflux from COS-7 cells.
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J Biol Chem
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Fehr, M.1
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Genetic engineering of an allosterically based glucose indicator protein for continuous glucose monitoring by fluorescence resonance energy transfer
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The authors develop of a glucose nanosensor for FRET detection that is based on bacterial periplasmic glucose-binding proteins.
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Ye K., Schultz J.S. Genetic engineering of an allosterically based glucose indicator protein for continuous glucose monitoring by fluorescence resonance energy transfer. Anal Chem. 75:2003;3119-3127 The authors develop of a glucose nanosensor for FRET detection that is based on bacterial periplasmic glucose-binding proteins.
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Development of a fluorescent nanosensor for ribose
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This work confirms that FRET-based nanosensors can be constructed on the basis of a variety of different bacterial periplasmic binding proteins. In this case, the ribose-binding protein is to develop a sensor that is then used to determine ribose homeostasis in living animal cells.
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Lager I., Fehr M., Frommer W.B., Lalonde S. Development of a fluorescent nanosensor for ribose. FEBS Lett. 553:2003;85-89 This work confirms that FRET-based nanosensors can be constructed on the basis of a variety of different bacterial periplasmic binding proteins. In this case, the ribose-binding protein is to develop a sensor that is then used to determine ribose homeostasis in living animal cells.
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FEBS Lett
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Conformational changes of three periplasmic receptors for bacterial chemotaxis and transport: The maltose-, glucose/galactose- and ribose-binding proteins
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Shilton B.H., Flocco M.M., Nilsson M., Mowbray S.L. Conformational changes of three periplasmic receptors for bacterial chemotaxis and transport: the maltose-, glucose/galactose- and ribose-binding proteins. J Mol Biol. 264:1996;350-363
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Single cell imaging of PI3K activity and glucose transporter insertion into the plasma membrane by dual color evanescent wave microscopy
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Tengholm A, Teruel MN, Meyer T: Single cell imaging of PI3K activity and glucose transporter insertion into the plasma membrane by dual color evanescent wave microscopy. Sci STKE, 2003 (169):pp.PL4.
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Sci STKE
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Feasibility study using surface-enhanced Raman spectroscopy for the quantitative detection of excitatory amino acids
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O'Neal P.D., Cote G.L., Motamedi M., Chen J., Lin W.C. Feasibility study using surface-enhanced Raman spectroscopy for the quantitative detection of excitatory amino acids. J Biomed Opt. 8:2003;33-39
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Lighting up cells with quantum dots
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Watson A, Wu X, Bruchez M: Lighting up cells with quantum dots. Biotechniques 2003, 34: 296-300, 302-293.
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Jaiswal J.K., Mattoussi H., Mauro J.M., Simon S.M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nature Biotech. 21:2003;47-51
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Nature Biotech
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Self-assembled nanoscale biosensors based on quantum dot FRET donors
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A must-read. Driven by the interest in determining cytosolic maltose levels in living yeast cells, Medintz and colleagues developed the first quantum dot FRET sensors for maltose by attaching the periplasmic maltose-binding protein to nanocrystals, and by measuring the FRET resulting from the displacement of a fluorescent cylodextrin derivate from the labeled MBP.
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Medintz I.L., Clapp A.R., Mattoussi H., Goldman E.R., Fisher B., Mauro J.M. Self-assembled nanoscale biosensors based on quantum dot FRET donors. Nat Mat. 2:2003;630-638 A must-read. Driven by the interest in determining cytosolic maltose levels in living yeast cells, Medintz and colleagues developed the first quantum dot FRET sensors for maltose by attaching the periplasmic maltose-binding protein to nanocrystals, and by measuring the FRET resulting from the displacement of a fluorescent cylodextrin derivate from the labeled MBP.
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Nat Mat
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Medintz, I.L.1
Clapp, A.R.2
Mattoussi, H.3
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Mauro, J.M.6
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Benson D.E., Conrad D.W., de Lorimier R.M., Trammell S.A., Hellinga H.W. Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins. Science. 293:2001;1641-1644
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Shrestha S., Salins L.L., Mark Ensor C., Daunert S. Rationally designed fluorescently labeled sulfate-binding protein mutants: evaluation in the development of a sensing system for sulfate. Biotechnol Bioeng. 78:2002;517-526
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Tsien RY: Imagining imaging's future. Nat Rev Mol Cell Biol 2003, Suppl:SS16-SS21.
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Marvin J.S., Hellinga H.W. Conversion of a maltose receptor into a zinc biosensor by computational design. Proc Natl Acad Sci USA. 98:2001;4955-4960
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Imaging of metabolites by using a fusion protein between a periplasmic binding protein and GFP derivatives: From a chimera to view of reality
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This review provides a perspective on the importance of FRET-based nanosensors among other metabolomics approaches.
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Stitt M. Imaging of metabolites by using a fusion protein between a periplasmic binding protein and GFP derivatives: from a chimera to view of reality. Proc Natl Acad Sci USA. 99:2002;9614-9616 This review provides a perspective on the importance of FRET-based nanosensors among other metabolomics approaches.
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Proc Natl Acad Sci USA
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Stitt, M.1
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