Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design

Journal of Biological Chemistry

Volumn 284, Issue 10, 2009, Pages 6455-6464

  Akerboom, Jasper ^a   Rivera, Jonathan D Vélez ^b   Rodríguez Guilbe, María M ^b   Malavé, Elisa C Alfaro ^b   Hernandez, Hector H ^c   Tian, Lin ^a   Hires, S Andrew ^a   Marvin, Jonathan S ^a   Looger, Loren L ^a   Schreiter, Eric R ^b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^c Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM BINDING; CALCIUM INDICATOR; CALCIUM SENSORS; CHEMICAL ENVIRONMENT; DARK STATE; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENCE SIGNALS; IMAGING EXPERIMENTS; INTER-DOMAIN; INTRACELLULAR CONCENTRATION; LOW SIGNAL-TO-NOISE RATIO; MONOMERIC FORMS; NETWORK ACTIVITIES; RATIONAL DESIGN; SENSOR FUNCTION; SIGNAL TO NOISE; SPECTROSCOPIC CHARACTERIZATION; STRUCTURAL DATA; X RAY CRYSTAL STRUCTURES;

AMINES; CALCIUM ALLOYS; CAMS; CHROMOPHORES; DYES; FLUORESCENCE; MACHINE DESIGN; PROTEINS; SENSORS; SIGNAL TO NOISE RATIO;

CALCIUM;

CALCIUM; CALMODULIN; GREEN FLUORESCENT PROTEIN; MYOSIN LIGHT CHAIN KINASE; CALCIUM BINDING PROTEIN; FLUORESCENT DYE; HYBRID PROTEIN; PEPTIDE;

ACTION POTENTIAL; ANIMAL TISSUE; ARTICLE; CALCIUM BINDING; CALCIUM TRANSPORT; CHROMATOPHORE; CONTROLLED STUDY; CRYSTAL STRUCTURE; FLUORESCENCE; MOLECULAR MECHANICS; MOLECULAR PROBE; MOUSE; MUTANT; NERVE CELL NETWORK; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ENGINEERING; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION; SPECTROSCOPY; X RAY CRYSTALLOGRAPHY; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; STRUCTURE ACTIVITY RELATION;

ANIMALS; CALCIUM; CALCIUM-BINDING PROTEINS; CRYSTALLOGRAPHY, X-RAY; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; MODELS, MOLECULAR; PEPTIDES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 65249106110     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M807657200     Document Type: Article

References (37)

1. Burgoyne, R. D. (2007) Nat. Rev. Neurosci. 8, 182-193
2. Jaffe, D. B., Johnston, D., Lasser-Ross, N., Lisman, J. E., Miyakawa, H., and Ross, W. N. (1992) Nature 357, 244-246
3. Muller, W., and Connor, J. A. (1991) Nature 354, 73-76
4. Yasuda, R., Nimchinsky, E. A., Scheuss, V., Pologruto, T. A., Oertner, T. G., Sabatini, B. L., and Svoboda, K. (2004) Sci. STKE 2004, pl5
5. Mank, M., and Griesbeck, O. (2008) Chem. Rev. 108, 1550-1564
6. Hires, S. A., Tian, L., and Looger, L. L. (2008) Brain Cell Biol. 36, 69-86
7. Wallace, D. J., Zum Alten Borgloh, S. M., Astori, S., Yang, Y., Bausen, M., Kugler, S., Palmer, A. E., Tsien, R. Y., Sprengel, R., Kerr, J. N., Denk, W., and Hasan, M. T. (2008) Nat. Methods 5, 797-804
8. Mank, M., Santos, A. F., Direnberger, S., Mrsic-Flogel, T. D., Hofer, S. B., Stein, V., Hendel, T., Reiff, D. F., Levelt, C., Borst, A., Bonhoeffer, T., Hubener, M., and Griesbeck, O. (2008) Nat. Methods 5, 805-811
9. Nakai, J., Ohkura, M., and Imoto, K. (2001) Nat. Biotechnol. 19, 137-141
10. Wang, Y., Guo, H. F., Pologruto, T. A., Hannan, F., Hakker, I., Svoboda, K., and Zhong, Y. (2004) J. Neurosci. 24, 6507-6514
11. Chalasani, S. H., Chronis, N., Tsunozaki, M., Gray, J. M., Ramot, D., Goodman, M. B., and Bargmann, C. I. (2007) Nature 450, 63-70
12. Naumann, E. A. (2005) Using G-CaMP 1.6 to Monitor Visually Evoked Synaptic Activity in Tectal Neurons in Vivo, PhD thesis, Universitat Konstanz, Konstanz, Germany
13. Tallini, Y. N., Ohkura, M., Choi, B. R., Ji, G., Imoto, K., Doran, R., Lee, J., Plan, P., Wilson, J., Xin, H. B., Sanbe, A., Gulick, J., Mathai, J., Robbins, J., Salama, G., Nakai, J., and Kotlikoff, M. I. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 4753-4758
14. Diez-Garcia, J., Matsushita, S., Mutoh, H., Nakai, J., Ohkura, M., Yokoyama, J., Dimitrov, D., and Knopfel, T. (2005) Eur. J. Neurosci. 22, 627-635
15. Ohkura, M., Matsuzaki, M., Kasai, H., Imoto, K., and Nakai, J. (2005) Anal. Chem. 77, 5861-5869
16. Mao, T., O'Connor, D. H., Scheuss, V., Nakai, J., and Svoboda, K. (2008) PLoS ONE 3, e1796
17. Rodriguez Guilbe, M. M., Alfaro Malave, E. C., Akerboom, J., Marvin, J. S., Looger, L. L., and Schreiter, E. R. (2008) Acta Crystallogr. Sect. F Struct. Biol. Crystallogr. Commun. 64, 629-631
18. Studier, F. W. (2005) Protein Expression Purif. 41, 207-234
19. Pflugrath, J. W. (1999) Acta Crystallogr. Sect. D Biol. Crystallogr. 55, 1718-1725
20. Otwinowski, Z., Minor, W., and Carter, C. W., Jr. (1997) Methods Enzymol. 276, 307-326
21. Leslie, A. G. W. (1992) Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography, Daresbury Laboratory, Daresbury, Warrington, UK
22. Collaborative Computational Project Number Four (1994) Acta Crystallogr. Sect. D Biol. Crystallogr. 50, 760-763
23. McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., and Read, R. J. (2007) J. Appl. Crystallogr. 40, 658-674
24. Kuboniwa, H., Tjandra, N., Grzesiek, S., Ren, H., Klee, C. B., and Bax, A. (1995) Nat. Struct. Biol. 2, 768-776
25. Emsley, P., and Cowtan, K. (2004) Acta Crystallogr. 60, 2126-2132
26. Murshudov, G. N., Vagin, A. A., and Dodson, E. J. (1997) Acta Crystallogr. 53, 240-255
27. Schuck, P. (2000) Biophys. J. 78, 1606-1619
28. Meador, W. E., Means, A. R., and Quiocho, F. A. (1992) Science 257, 1251-1255
29. Zhang, M., Tanaka, T., and Ikura, M. (1995) Nat. Struct. Biol. 2, 758-767
30. Yang, F., Moss, L. G., and Phillips, G. N., Jr. (1996) Nat. Biotechnol. 14, 1246-1251
31. Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996) Science 273, 1392-1395
32. Jung, G., Wiehler, J., and Zumbusch, A. (2005) Biophys. J. 88, 1932-1947
33. Kummer, A. D., Wiehler, J., Rehaber, H., Kompa, C., Steipe, B., and Michel-Beyerle, M. E. (2000) J. Phys. Chem. B 104, 4791-4798
34. Souslova, E. A., Belousov, V. V., Lock, J. G., Stromblad, S., Kasparov, S., Bolshakov, A. P., Pinelis, V. G., Labas, Y. A., Lukyanov, S., Mayr, L. M., and Chudakov, D. M. (2007) BMC Biotechnol. 7, 37
35. Brejc, K., Sixma, T. K., Kitts, P. A., Kain, S. R., Tsien, R. Y., Ormo, M., and Remington, S. J. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 2306-2311
36. Elsliger, M. A., Wachter, R. M., Hanson, G. T., Kallio, K., and Remington, S. J. (1999) Biochemistry 38, 5296-5301
37. Word, J. M., Lovell, S. C., LaBean, T. H., Taylor, H. C., Zalis, M. E., Presley, B. K., Richardson, J. S., and Richardson, D. C. (1999) J. Mol. Biol. 285, 1711-1733