Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9

Biotechnology and Bioengineering

Volumn 114, Issue 1, 2017, Pages 172-183

  Chung, Mu En ^a   Yeh, I Hsin ^a   Sung, Li Yu ^a   Wu, Meng Ying ^a   Chao, Yun Peng ^b   Ng, I Son ^c   Hu, Yu Chen ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b FENG CHIA UNIVERSITY   (Taiwan)

^c NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

CRISPR Cas9; double strand break; E. coli; genome editing; integration; metabolic engineering

Indexed keywords

CELL DEATH; CHROMOSOMES; DNA; EFFICIENCY; ESCHERICHIA COLI; GENES; METABOLIC ENGINEERING; METABOLISM; NUCLEIC ACIDS;

CHROMOSOMAL INTEGRATION; CRISPR/CAS9; DOUBLE STRAND BREAKS; E. COLI; EXPERIMENTAL CONDITIONS; HOMOLOGOUS RECOMBINATION; PRECISE INTEGRATION; RECOMBINEERING;

INTEGRATION;

CELL DEATH; CELL SURVIVAL; CHROMOSOME; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA REPAIR; DNA STRAND; EXPERIMENTAL MODEL; GENE DISRUPTION; GENOME; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; CRISPR CAS SYSTEM; DOUBLE STRANDED DNA BREAK; ESCHERICHIA COLI; GENE EDITING; GENETICS; METABOLISM; PLASMID; PROCEDURES; SOS RESPONSE (GENETICS);

DNA;

CELL SURVIVAL; CRISPR-CAS SYSTEMS; DNA; DNA BREAKS, DOUBLE-STRANDED; ESCHERICHIA COLI; GENE EDITING; METABOLIC ENGINEERING; PLASMIDS; SOS RESPONSE (GENETICS);

EID: 84980351609     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26056     Document Type: Article

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