Establishment of functional genomics pipeline in epiblast-like tissue by combining transcriptomic analysis and gene knockdown/knockin/knockout, using RNA interference and CRISPR/Cas9

Human Gene Therapy

Volumn 27, Issue 6, 2016, Pages 436-450

  Takata, Nozomu ^a   Sakakura, Eriko ^a   Kasukawa, Takeya ^b   Sakuma, Tetsushi ^c   Yamamoto, Takashi ^c   Sasai, Yoshiki ^a  

^a RIKEN Center for Biosystems Dynamics Research   (Japan)

^b RIKEN   (Japan)

^c HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR; BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR TFE3; CADHERIN; CD31 ANTIGEN; INTEGRIN LINKED KINASE; LAMININ; MITOGEN ACTIVATED PROTEIN KINASE; OCTAMER TRANSCRIPTION FACTOR 4; ORGANIC CATION TRANSPORTER 3; SHORT HAIRPIN RNA; TRANSCRIPTION FACTOR OTX2; UNCLASSIFIED DRUG; UVOMORULIN; FGF5 PROTEIN, MOUSE; FIBROBLAST GROWTH FACTOR 5;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOINFORMATICS; CELL SURFACE; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9; ECTODERM; EMBRYO; EMBRYONIC STEM CELL; ENDODERM; EXTRACELLULAR MATRIX; FLUORESCENCE ACTIVATED CELL SORTING; FUNCTIONAL GENOMICS; GEL ELECTROPHORESIS; GENE EXPRESSION; GENE SILENCING; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MICROARRAY ANALYSIS; MOUSE; NEUROECTODERM; NONHUMAN; RNA INTERFERENCE; TRANSCRIPTOMICS; TRANSPOSON; UPREGULATION; ANIMAL; CELL DIFFERENTIATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOLOGY; DEFICIENCY; GENE EXPRESSION PROFILING; GENETICS; GENOME; GENOMICS; GERM LAYER; KNOCKOUT MOUSE; METABOLISM; PLURIPOTENT STEM CELL; PROCEDURES;

ANIMALS; CELL DIFFERENTIATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; EMBRYONIC STEM CELLS; FIBROBLAST GROWTH FACTOR 5; GENE EXPRESSION PROFILING; GENOME; GENOMICS; GERM LAYERS; MICE; MICE, KNOCKOUT; PLURIPOTENT STEM CELLS; RNA INTERFERENCE;

EID: 84975525711     PISSN: 10430342     EISSN: 15577422     Source Type: Journal    
DOI: 10.1089/hum.2015.148     Document Type: Article

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