Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis

Microbial Cell Factories

Volumn 15, Issue 1, 2016, Pages

  Guan, Chengran ^a   Cui, Wenjing ^a   Cheng, Jintao ^a   Zhou, Li ^a   Liu, Zhongmei ^a   Zhou, Zhemin ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Auto inducible; Bacillus subtilis; Expression system; High cell density fermentation; Promoter engineering

Indexed keywords

AMINOPEPTIDASE; GREEN FLUORESCENT PROTEIN; NATTOKINASE; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CHROMOSOME; BACTERIAL STRAIN; BACTERIUM MUTANT; BATCH FERMENTATION; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; FERMENTATION OPTIMIZATION; GENE EXPRESSION SYSTEM; GENE LIBRARY; GENE OVEREXPRESSION; GENETIC ENGINEERING; GENETIC IMPROVEMENT; HETEROLOGOUS EXPRESSION; INDUSTRIAL PRODUCTION; NONHUMAN; P23 PROMOTER; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; SPOROGENESIS; SRFA PROMOTER; STABLE EXPRESSION; WILD TYPE; FERMENTATION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MICROBIOLOGY; MOLECULAR CLONING; PROCEDURES; QUORUM SENSING; TRANSGENIC ORGANISM;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BIOREACTORS; CLONING, MOLECULAR; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; INDUSTRIAL MICROBIOLOGY; ORGANISMS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; QUORUM SENSING;

EID: 84964397449     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0464-0     Document Type: Article

References (45)

1. Terpe K. Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 2006;72:211-22.
2. Vavrova L, Muchova K, Barak I. Comparison of different Bacillus subtilis expression systems. Res Microbiol. 2010;161:791-7.
3. Commichau FM, Alzinger A, Sande R, Bretzel W, Meyer FM, Chevreux B, Wyss M, Hohmann HP, Pragai Z. Overexpression of a non-native deoxyxylulose-dependent vitamin B6 pathway in Bacillus subtilis for the production of pyridoxine. Metab Eng. 2014;25:38-49.
4. Zafar M, Ahmed S, Khan MI, Jamil A. Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli. Mol Biol Rep. 2014;41:3295-302.
5. Zaghloul TI, Abdelaziz A, Mostafa MH. High level of expression and stability of the cloned alkaline protease (aprA) gene in Bacillus subtilis. Enzyme Microb Technol. 1994;16:534-7.
6. Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO. Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC. Biotechnol Bioeng. 2012;109:2349-56.
7. Chen PT, Shaw JF, Chao YP, Ho THD, Yu SM. Construction of chromosomally located T7 expression system for production of heterologous secreted proteins in Bacillus subtilis. J Agri Food Chem. 2010;58:5392-9.
8. Bhavsar AP, Zhao X, Brown ED. Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant. Appl Environ Microbiol. 2001;67:403-10.
9. Bongers RS, Veening JW, Van Wieringen M, Kuipers OP, Kleerebezem M. Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: strict control of gene expression by addition of subtilin. Appl Environ Microbiol. 2005;71:8818-24.
10. Nijland R, Lindner C, van Hartskamp M, Hamoen LW, Kuipers OP. Heterologous production and secretion of clostridium perfringens beta-toxoid in closely related Gram-positive hosts. J Biotechnol. 2007;127:361-72.
11. Kerovuo J, von Weymarn N, Povelainen M, Auer S, Miasnikov A. A new efficient expression system for Bacillus and its application to production of recombinant phytase. Biotechnol Lett. 2000;22:1311-7.
12. Wenzel M, Muller A, Siemann-Herzberg M, Altenbuchner J. Self-inducible Bacillus subtilis expression system for reliable and inexpensive protein production by high-cell-density fermentation. Appl Environ Microbiol. 2011;77:6419-25.
13. Trang TPP, Nguyen D, Schumann W. Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements. J Biotechnol. 2012;157:167-72.
14. Jan J, Valle F, Bolivar F, Merino E. Construction of protein overproducer strains in Bacillus subtilis by an integrative approach. Appl Microbiol Biot. 2001;55:69-75.
15. Lee SJ, Pan JG, Park SH, Choi SK. Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis. J Biotechnol. 2010;149:16-20.
16. Li MJ, Wang JS, Geng YP, Li YK, Wang Q, Liang QF, Qi QS. A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli. Microb Cell Fact. 2012;11:19.
17. Kang HK, Jang JH, Shim JH, Park JT, Kim YW, Park KH. Efficient constitutive expression of thermostable 4-alpha-glucanotransferase in Bacillus subtilis using dual promoters. World J Microb Biot. 2010;26:1915-8.
18. Guan CR, Cui WJ, Cheng JT, Zhou L, Guo JL, Hu X, Xiao GP, Zhou ZM. Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015;14:150.
19. Hamoen LW. Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 2003;149:9-17.
20. Schneider KB, Palmer TM, Grossman AD. Characterization of comQ and comX, two genes required for production of ComX pheromone in Bacillus subtilis. J Bacteriol. 2002;184:410-9.
21. Roggiani M, Dubnau D. ComA, a phosphorylated response regulator protein of Bacillus subtilis, binds to the promoter region of srfA. J Bacteriol. 1993;175:3182-7.
22. Phanaksri T, Luxananil P, Panyim S, Tirasophon W. Synergism of regulatory elements in sigma(B)- and sigma(A)-dependent promoters enhances recombinant protein expression in Bacillus subtilis. J Biosci Bioeng. 2015;120:470-5.
23. Gao X, Cui W, Tian Y, Zhou Z. Over-expression, secretion, biochemical characterisation, and structure analysis of Bacillus subtilis aminopeptidase. J Sci Food Agric. 2013;93:2810-5.
24. Ben Khedher S, Zouari N, Messaddeq N, Schultz P, Jaoua S. Overproduction of delta-endotoxins by sporeless Bacillus thuringiensis mutants obtained by nitrous acid mutagenesis. Curr Microbiol. 2011;62:38-43.
25. Claverys JP, Prudhomme M, Martin B. Induction of competence regulons as a general response to stress in gram-positive bacteria. Annu Rev Microbiol. 2006;60:451-75.
26. Lopez D, Kolter R. Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis. FEMS Microbiol Rev. 2010;34:134-49.
27. Tyo KE, Ajikumar PK, Stephanopoulos G. Stabilized gene duplication enables long-term selection-free heterologous pathway expression. Nat Biotechnol. 2009;27:760-5.
28. Tyo KEJ, Ajikumar PK, Stephanopoulos G. Stabilized gene duplication enables long-term selection-free heterologous pathway expression. Nat Biotechnol. 2009;27:760-5.
29. Alper H, Fischer C, Nevoigt E, Stephanopoulos G. Tuning genetic control through promoter engineering. Proc Natl Acad Sci USA. 2005;102:12678-83.
30. Bakke I, Berg L, Aune TE, Brautaset T, Sletta H, Tondervik A, Valla S. Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression. Appl Environ Microbiol. 2009;75:2002-11.
31. Wu SM, Feng C, Zhong J, Huan LD. Enhanced production of recombinant nattokinase in Bacillus subtilis by promoter optimization. World J Microb Biot. 2011;27:99-106.
32. Phan TT, Nguyen HD, Schumann W. Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements. J Biotechnol. 2012;157:167-72.
33. Voskuil MI, Chambliss GH. The 16 region of Bacillus subtilis and other gram-positive bacterial promoters. Nucleic Acids Res. 1998;26:3584-90.
34. Helmann JD. Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res. 1995;23:2351-60.
35. Haldenwang WG. The sigma factors of Bacillus subtilis. Microbiol Rev. 1995;59:1-30.
36. Cosby WM, Vollenbroich D, Lee OH, Zuber P. Altered srf expression in Bacillus subtilis resulting from changes in culture pH is dependent on the Spo0K oligopeptide permease and the ComQX system of extracellular control. J Bacteriol. 1998;180:1438-45.
37. Li MZ, Elledge SJ. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods. 2007;4:251-6.
38. Green MR, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press; 2012.
39. Bloor AE, Cranenburgh RM. An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes. Appl Environ Microbiol. 2006;72:2520-5.
40. Yan X, Yu HJ, Hong Q, Li SP. Cre/lox system and PCR-based genome engineering in Bacillus subtilis. Appl Environ Microbiol. 2008;74:5556-62.
41. Miyazaki K, Takenouchi M. Creating random mutagenesis libraries using megaprimer PCR of whole plasmid. Biotechniques. 1033;2002:33.
42. Botella E, Fogg M, Jules M, Piersma S, Doherty G, Hansen A, Denham EL, Le Chat L, Veiga P, Bailey K, et al. pBaSysBioll: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis. Microbiology-Sgm. 2010;156:1600-8.
43. Gao X, Liu Z, Cui W, Zhou L, Tian Y, Zhou Z. Enhanced thermal stability and hydrolytic ability of Bacillus subtilis aminopeptidase by removing the thermal sensitive domain in the non-catalytic region. PLoS One. 2014;9:e92357.
44. Suwanmanon K, Hsieh PC. Isolating Bacillus subtilis and optimizing its fermentative medium for GABA and nattokinase production. Cyta-J Food. 2014;12:282-90.
45. Hartl B, Wehrl W, Wiegert T, Homuth G, Schumann W. Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes. J Bacteriol. 2001;183:4393.