Random mutagenesis of the Pm promoter as a powerful strategy for improvement of recombinant-gene expression

Applied and Environmental Microbiology

Volumn 75, Issue 7, 2009, Pages 2002-2011

  Bakke, Ingrid ^a   Berg, Laila ^a   Aune, Trond Erik Vee ^a   Brautaset, Trygve ^b   Sletta, Håvard ^b   Tøndervik, Anne ^b   Valla, Svein ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b SINTEF MATERIALS AND CHEMISTRY   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

E. COLI; FIREFLY LUCIFERASE; FUNCTIONAL ELEMENTS; GRAM-NEGATIVE SPECIES; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTORS; HIGH CELL DENSITIES; LACTAMASE; LOW FREQUENCIES; MULTIPLE POINTS; MUTANT LIBRARIES; OPTIMIZED PROCESS; PROMOTER ACTIVITIES; PROMOTER SEQUENCES; PROTEIN EXPRESSIONS; RANDOM MUTAGENESIS; RECOMBINANT PROTEINS; REPORTER GENES; TRANSCRIPTION START SITES; WILD TYPES;

ENCODING (SYMBOLS); ESCHERICHIA COLI; MUTAGENESIS; PLANTS (BOTANY); PROTEINS;

TRANSCRIPTION;

BETA LACTAMASE; FIREFLY LUCIFERASE; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; PHOSPHOGLUCOMUTASE;

COLIFORM BACTERIUM; CULTIVATION; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; OPTIMIZATION; PROTEIN; RECOMBINATION;

ARTICLE; BACTERIAL STRAIN; ESCHERICHIA COLI; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; MUTAGENESIS; NONHUMAN; POINT MUTATION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PSEUDOMONAS; RECOMBINANT GENE; REPORTER GENE;

BASE SEQUENCE; BETA-LACTAMASES; DNA MUTATIONAL ANALYSIS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENES, REPORTER; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; LUCIFERASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PHOSPHOGLUCOMUTASE; POINT MUTATION; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; SEQUENCE DELETION; UP-REGULATION;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 63849118218     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02315-08     Document Type: Article

References (36)

1. Berg, L., R. Lale, I. Bakke, and S. Valla. The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5′-untranslated part of mRNA. Microb. Biotechnol, in press.
2. Blatny, J. M., T. Brautaset, H. C. Winther-Larsen, K. Haugan, and S. Valla. 1997. Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon. Appl. Environ. Microbiol. 63:370-379.
3. Blatny, J. M., T. Brautaset, H. C. Winther-Larsen, P. Karunakaran, and S. Valla. 1997. Improved broad-host-range RK2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria. Plasmid 38:35-51.
4. Brautaset, T., R. Lale, and S. Valla. 2009. Positively regulated bacterial expression systems. Rev. Microb. Biotechnol. 2:15-30.
5. Brautaset, T., S. B. Petersen, and S. Valla. 2000. In vitro determined kinetic properties of mutant phosphoglucomutases and their effect on sugar catab-olism in Escherichia coli. Metab. Eng. 2:104-114.
6. Brautaset, T., R. Standal, E. Fjaervik, and S. Valla. 1994. Nucleotide sequence and expression analysis of the Acetobacter xylinum phosphoglu-comutase gene. Microbiology 140:1183-1188.
7. de Wet, J. R., K. V. Wood, D. R. Helínski, and M. DeLuca. 1985. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Froc. Natl. Acad. Sci., USA 82:7870-7873.
8. Domínguez-Cuevas, P., J. E. González-Pastor, S. Marqués, J. L. Ramos, and V. de Lorenzo. 2006. Transcriptional tradeoff between metabolic and stress-response programs in Pseudomonas putida KT2440 cells exposed to toluene. J. Biol. Chem. 281:11981- 11991.
9. 32. J. Biol. Chem. 280:41315-41323.
10. Fjævik, E., K. Frydenlund, S. Valla, Y. Huggriat, and M. Benziman. 1991. Complementation of cellulose-negative mutants of Acetobacter xylinum by the cloned structural gene for phosphoglucomutase. FEMS Microbiol. Lett. 77:325-330.
11. s RNA polymerase. Mol. Microbiol. 42:939-954.
12. González-Pérez, M. M., S. Marqués, P. Domínguez-Cuevas, and J. L. Ramos. 2002. XylS activator and RNA polymerase binding sites at the Pm promoter overlap. FEBS Lett. 519:117-122.
13. Inouye, S., A. Nakazawa, and T. Nakazawa. 1981. Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS. J. Bacteriol. 148:413-18.
14. Jensen, P. R., and K. Hammer. 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol. 64:82-87.
15. s-dependent genes and identification of their promoter sequences. J. Bacteriol. 186:7186-7195.
16. Lewis, D. E. A., and S. Adhya. 2004. Axiom of determining transcription start points by RNA polymerase in Escherichia coli. Mol. Microbiol. 54:692-701.
17. lac pro-moter hyperactive and cAMP receptor protein-independent. Proc. Natl. Acad. Sci. USA 101:6911-6916.
18. Ma, D., D. N. Cook, M. Alberti, N. G. Pon, H. Nikaido, and J. E. Hearst. 1995. Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli. Mol. Microbiol. 16:45-55.
19. 38 depending on the growth phase. Mol. Microbiol. 31:1105-1113.
20. Mermod, N., J. L. Ramos, P. R. Lehrbach, and K. N. Timmis. 1986. Vector for regulated expression of cloned genes in a wide range of gram-negative bacteria. J. Bacteriol. 167:447-454.
21. Miksch, G., F. Bettenworth, K. Friehs, E. Flaschel, A. Saalbach, T. Twell-mann, and T. W. Nattkemper. 2005. Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. J. Biotechnol. 120:25-37.
22. Mitchell, J. E., D. Zheng, S. J. W. Busby, and S. D. Minchin. 2003. Identification and analysis of "extended -10" promoters in Escherichia coli. Nucleic Acids Res. 31:4689-4695.
23. Murakami, K. S., S. Masuda, E. A. Campbell, O. Muzzin, and S. A. Darst. 2002. Structural basis of transcription initiation: an RNA polymerase holo-enzyme-DNA complex. Science 296:1285-1290.
24. Ramos, J. L., M. Gonzalez-Carrero, and K, Timmis. 1988. Broad-host range expression vectors containing manipulated meta-cleavage pathway regulatory elements of the TOL plasmid. FEBS Lett. 226:241-246.
25. Ramos, J. L., S. Marqués, and K. N. Timmis. 1997. Transcriptional control of the Pseudomonas TOL plasrnid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annu. Rev. Microbiol. 51:341-372.
26. Ramos, J. L., A. Stolz, W. Reineke, and K. N. Timmis. 1986. Altered effector specificities in regulators of gene expression: TOL plasrnid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteria. Proc. Natl. Acad. Sci. USA 83:8467-8471.
27. Sailer, F. C., B. M. Meberg, and K. D. Young. 2003. β-Lactam induction of colanic acid gene expression in Escherichia coli. FEMS Microbiol. Lett. 226:245-249.
28. Santos, P. M., I. D. Bartolo, J. M. Blatny, E. Zennaro, and S. Valla. 2001. New broad-host-range promoter probe vectors based on the plasrnid RK2 replicon. FEMS Microbiol. Lett. 195:91-96.
29. Sikkema, J., J. A. de Bont, and B. Poolman. 1995. Mechanisms of membrane toxicity of hydrocarbons. Microbiol. Rev. 59:201-222.
30. Sletta, H., A. Nedal, T. E. V. Aune, H. Hellebust, S. Hakvåg, R. Aune, T. E. Ellingsen, S. Valla, and T. Brautaset. 2004. Broad-host-range plasrnid pJB658 can be used for industrial level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli. Appl. Environ. Microbiol. 70:7033-7039.
31. Sletta, H., A. Tøndervik, S. Hakvåg, T. E. Vee Aune, A. Nedal, R. Aune, G. Evensen, S. Valla, T. E. Ellingsen, and T. Brautaset. 2007. The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli. Appl. Environ. Microbiol. 73: 906-912.
32. Uhlin, B. E., and K. Nordstrom. 1977. R plasrnid gene dosage effects in Escherichia coli. K-12: copy mutants of the R plasrnid Rldrd-19. Plasrnid 1:1-7.
33. Walker, K. A., and R. Osuna. 2002. Factors affecting start site selection at the Escherichia coli fis promoter. J. Bacteriol. 184:4783-4791.
34. Winther-Larsen, H. C., J. M. Blatny, B. Valand, T. Brautaset, and S. Valla. 2000. Pm promoter expression mutants and their use in broad-host-range RK2 plasrnid vectors. Metab. Eng. 2:92-103.
35. Winther-Larsen, H. C., K. D. Josefsen, T. Brautaset, and S. Valla. 2000. Parameters affecting gene expression from the Pm promoter in gram-negative bacteria. Metab. Eng. 2:79-91.
36. Young, B. A., and T. M. Gruber. 2002. Views on transcription initiation. Cell 109:417-420.