Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements

Journal of Biotechnology

Volumn 157, Issue 1, 2012, Pages 167-172

  Phan, Trang Thi Phuong ^a,b   Nguyen, Hoang Duc ^a,b   Schumann, Wolfgang ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b UNIVERSITY OF NATURAL SCIENCES   (Viet Nam)

Author keywords

35 and 10 elements; ITPG induction; UP element

Indexed keywords

BACILLUS SUBTILIS; BACILLUS SUBTILIS CELLS; CELLULAR PROTEINS; CONSERVED REGIONS; EXPERIMENTAL DATA; EXPRESSION SYSTEM; INDUCIBLE PROMOTER; ITPG-INDUCTION; PROMOTER ELEMENTS; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; UP ELEMENT;

BACILLI; BACTERIOLOGY; OPTIMIZATION;

PROTEINS;

BACTERIAL PROTEIN; BETA GALACTOSIDASE; BGAB REPORTER PROTEIN; GROESL PROTEIN; PLASMID VECTOR; UNCLASSIFIED DRUG;

ARTICLE; BACILLI; BACILLUS SUBTILIS; CELL LEVEL; DNA FLANKING REGION; DNA SEQUENCE; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE LOCUS; NONHUMAN; NORTHERN BLOTTING; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN CONTENT; TRANSCRIPTION INITIATION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BLOTTING, NORTHERN; CHAPERONINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; ISOPROPYL THIOGALACTOSIDE; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

BACILLUS SUBTILIS;

EID: 84855223196     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.10.006     Document Type: Article

References (39)

1. Aiyar S.E., Gourse R.L., Ross W. Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase alpha subunit. Proc. Natl. Acad. Sci. U.S.A. 1998, 95:14652-14657.
2. Baneyx F. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 1999, 10:411-421.
3. Bongers R.S., Veening J.W., Van W.M., Kuipers O.P., Kleerebezem M. Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: strict control of gene expression by addition of subtilin. Appl. Environ. Microbiol. 2005, 71:8818-8824.
4. Brautaset T., Lale R., Valla S. Positively regulated bacterial expression systems. Microbiol. Biotechnol. 2009, 2:15-30.
5. Caramori T., Galizzi A. The UP element of the promoter for the flagellin gene, hag, stimulates transcription from both SigD- and SigA-dependent promoters in Bacillus subtilis. Mol. Gen. Genet. 1998, 258:385-388.
6. Chen P.T., Shaw J.F., Chao Y.P., David Ho T.H., Yu S.M. Construction of chromosomally located T7 expression system for production of heterologous secreted proteins in Bacillus subtilis. J. Agric. Food Chem. 2010, 58:5392-5399.
7. Geissendorfer M., Hillen W. Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements. Appl. Microbiol. Biotechnol. 1990, 33:657-663.
8. Gilbert W., Müller-Hill B. Isolation of the lac repressor. Proc. Natl. Acad. Sci. U.S.A. 1966, 56:1891-1898.
9. Hannig G., Makrides S.C. Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol. 1998, 16:54-60.
10. Helmann J.D. Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res. 1995, 23:2351-2360.
11. Henkin T.M., Sonenshein A.L. Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis. Mol. Gen. Genet. 1987, 209:467-474.
12. Homuth G., Heinemann M., Zuber U., Schumann W. The genes of lepA and hemN form a bicistronic operon in Bacillus subtilis. Microbiology 1996, 142:1641-1649.
13. Homuth G., Masuda S., Mogk A., Kobayashi Y., Schumann W. The dnaK operon of Bacillus subtilis is heptacistronic. J. Bacteriol. 1997, 179:1153-1164.
14. Jan J., Valle F., Bolivar F., Merino E. Construction of protein overproducer strains in Bacillus subtilis by an integrative approach. Appl. Microbiol. Biotechnol. 2001, 55:69-75.
15. Kim L., Mogk A., Schumann W. A xylose-inducible Bacillus subtilis integration vector and its application. Gene 1996, 181:71-76.
16. Lee J.K., Edwards C.W., Hulett F.M. Bacillus licheniformis APase I gene promoter: a strong well-regulated promoter in B. subtilis. J. Gen. Microbiol. 1991, 137:1127-1133.
17. Lee S.J., Pan J.G., Park S.H., Choi S.K. Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis. J. Biotechnol. 2010, 149:16-20.
18. lac promoter hyperactive and cAMP receptor protein-independent. Proc. Natl. Acad. Sci. U.S.A. 2004, 101:6911-6916.
19. Meijer W.J., Salas M. Relevance of UP elements for three strong Bacillus subtilis phage phi29 promoters. Nucleic Acids Res. 2004, 32:1166-1176.
20. Mergulhao F.J., Summers D.K., Monteiro G.A. Recombinant protein secretion in Escherichia coli. Biotechnol. Adv. 2005, 23:177-202.
21. Miroux B., Walker J.E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 1996, 260:289-298.
22. Nguyen H.D., Nguyen Q.A., Ferreira R.C., Ferreira L.C., Tran L.T., Schumann W. Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability. Plasmid 2005, 54:241-248.
23. Nguyen H.D., Phan T.T.P., Schumann W. Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis. Curr. Microbiol. 2007, 55:89-93.
24. Phan T.T., Nguyen H.D., Schumann W. Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis. Protein Expr. Purif. 2010, 71:174-178.
25. Phan T.T., Schumann W. Development of a glycine-inducible expression system for Bacillus subtilis. J. Biotechnol. 2007, 128:486-499.
26. Phan T.T.P., Nguyen H.D., Schumann W. Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr. Purif. 2006, 46:189-195.
27. Pohl S., Harwood C.R. Heterologous protein secretion by Bacillus species: from the cradle to the grave. Adv. Appl. Microbiol. 2010, 73:1-25.
28. Saito H., Shibata T., Ando T. Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol. Gen. Genet. 1979, 170:117-122.
29. Schallmey M., Singh A., Ward O.P. Developments in the use of Bacillus species for industrial production. Can. J. Microbiol. 2004, 50:1-17.
30. Schmidt A., Schiesswohl M., Völker U., Hecker M., Schumann W. Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis. J. Bacteriol. 1992, 174:3993-3999.
31. Schumann W. Production of recombinant proteins in Bacillus subtilis. Adv. Appl. Microbiol. 2007, 62:137-189.
32. Titok M.A., Chapuis J., Selezneva Y.V., Lagodich A.V., Prokulevich V.A., Ehrlich S.D., Janniere L. Bacillus subtilis soil isolates: plasmid replicon analysis and construction of a new theta-replicating vector. Plasmid 2003, 49:53-62.
33. Valdez-Cruz N.A., Caspeta L., Perez N.O., Ramirez O.T., Trujillo-Roldan M.A. Production of recombinant proteins in E coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters. Microb. Cell Fact 2010, 9:18.
34. Voskuil M.I., Chambliss G.H. The -16 region of Bacillus subtilis and other gram-positive bacterial promoters. Nucleic Acids Res. 1998, 26:3584-3590.
35. Westers L., Westers H., Quax W.J. Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim. Biophys. Acta 2004, 1694:299-310.
36. Wiegert T., Homuth G., Versteeg S., Schumann W. Alkaline shock induces the Bacillus subtilis sigma(W) regulon. Mol. Microbiol. 2001, 41:59-71.
37. Yamamoto H., Murata M., Sekiguchi J. The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis. Mol. Microbiol. 2000, 37:898-912.
38. Yansura D.G., Henner D.J. Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 1984, 81:439-443.
39. Zweers J.C., Barak I., Becher D., Driessen A.J., Hecker M., Kontinen V.P., Saller M.J., Vavrova L., Van Dijl J.M. Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes. Microb. Cell Fact 2008, 7:10.