Enhanced production of recombinant nattokinase in Bacillus subtilis by promoter optimization

World Journal of Microbiology and Biotechnology

Volumn 27, Issue 1, 2011, Pages 99-106

  Wu, Shu Ming ^a   Feng, Chi ^a   Zhong, Jin ^a   Huan, Lian Dong ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Bacillus subtilis; Enhanced production; Nattokinase; Promoter; Subtilisin NAT

Indexed keywords

BACILLUS SUBTILIS; ENHANCED PRODUCTION; NATTOKINASE; PROMOTER; SUBTILISIN;

BACILLI; BACTERIOLOGY; DISEASE CONTROL; GENES;

ORGANIC POLYMERS;

BACILLUS SUBTILIS;

EID: 79953326595     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-010-0432-5     Document Type: Article

References (21)

1. Akamatsu T, Taguchi H, Okada H (2000) A simple and rapid extraction of high molecular weight chromosomal DNA from Bacillus subtilis protoplasts for cosmid cloning and interspecific transformation. Biosci Biotechnol Biochem 64: 1082-1083.
2. Astrup T, Mullertz S (1952) The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 40: 346-351.
3. Chen P, Chao Y (2006) Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors. Biotechnol Lett 28: 1595-1600.
4. Chen P, Chiang C, Chao Y (2007a) Strategy to approach stable production of recombinant nattokinase in Bacillus subtilis. Biotechnol Prog 23(4): 808-813.
5. Chen P, Chiang C, Chao Y (2007b) Medium optimization for the production of recombinant nattokinase by Bacillus subtilis using response surface methodology. Biotechnol Prog 23(6): 1327-1332.
6. de Ruyter PG, Kuipers OP, de Vos WM (1996) Controlled gene expression system for Lactococcus lactis with the food-grade inducer nisin. Appl Environ Microbiol 62: 3662-3667.
7. Fujita M, Hong K, Nishimuro S (1995) Characterization of nattokinase-degraded products from human fibrinogen or cross-linked fibrin. Fibrinolysis 9: 157-164.
8. Helmann JD (1995) Compilation and analysis of Bacillus subtilis σA-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res 23: 2351-2360.
9. Horton R, Hunt H, Ho S, Pullen J, Pease L (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77: 61-68.
10. Ishiwa H, Shibahara H (1985) New shuttle vectors for Escherichia coli and Bacillus subtilis. II. Plasmid pHY300PLK, a multipurpose cloning vector with a polylinker, derived from pHY460. Jpn J Genet 60: 235-243.
11. Jan J, Valle F, Bolivar F, Merino E (2001) Construction of protein overproducer strains in Bacillus subtilis by an integrative approach. Appl Microbiol Biotechnol 55: 69-75.
12. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907.
13. Kawamura F, Doi RH (1984) Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J Bacteriol 160: 442-444.
14. Liu B, Song H (2002) Molecular cloning and expression of nattokinase gene in Bacillus subtilis. Acta Biochim Biophys Sin 34: 338-340.
15. Sumi H, Hamada N, Tsushima H (1987) A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto: a typical and popular soybean food in the Japanese diet. Experientia 43: 1110-1111.
16. Tai M (2006) Nattokinase for prevention of thrombosis. Am J Health Syst Pharm 63: 1121-1123.
17. Wei W, Xiang H, Tan H (2002) Two tandem promoters to increase gene expression in Lactococcus lactis. Biotechnol Lett 24: 1669-1672.
18. Widner B, Thomas M, Sternberg D, Lammon D, Behr R, Sloma A (2000) Development of marker-free stains of Bacillus subtilis capable of secreting high levels of industrial enzymes. J Ind Microbiol Biotechnol 25: 201-212.
19. Wu S, Feng C, Zhong J, Huan L (2007) Roles of S3 site residues of nattokinase on its activity and substrate specificity. J Biochem 142: 357-364.
20. Xue GP, Johason JS, Dalrymple BP (1999) High osmolarity improves the electro-transformation efficiency of gram-positive bacteria Bacillus subtilis and Bacillus licheniformis. J Microbiol Methods 34: 183-191.
21. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.