Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

Journal of Biotechnology

Volumn 149, Issue 1-2, 2010, Pages 16-20

  Lee, Su Jin ^a   Pan, Jae Gu ^a   Park, Seung Hwan ^a   Choi, Soo Keun ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Bacillus subtilis; Bacillus thuringiensis cry3Aa; Expression system; Promoter modification

Indexed keywords

AUTOINDUCTION; BACILLUS SUBTILIS; BACILLUS THURINGIENSIS; CONSENSUS SEQUENCE; CRY GENES; CRYSTAL PROTEINS; DRY CELLS; EXPRESSION LEVELS; EXPRESSION SYSTEM; EXTRACELLULAR ENZYMES; HIGH LEVEL EXPRESSION; HIGHER YIELD; PROMOTER SEQUENCES; PROTEIN EXPRESSIONS; STATIONARY PHASE; SUBTILIS;

BACTERIOLOGY; PROTEINS;

GENE EXPRESSION;

ACETIC ACID; ALCOHOL; BETA GALACTOSIDASE; CELLULASE; CRY1AC TOXIN; FURFURAL; PROPIDIUM IODIDE;

APRE GENE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BATCH FERMENTATION; CELL SIZE; CELL VIABILITY; CRY11AA GENE; CRY15AA GENE; CRY1AA GENE; CRY3AA GENE; CYT1AA GENE; ENZYME REGULATION; FUSION GENE; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENOTYPE; LACZ GENE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; TEMPERATURE;

BACILLUS SUBTILIS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; ENDOTOXINS; GENE EXPRESSION REGULATION, BACTERIAL; HEMOLYSIN PROTEINS; PROMOTER REGIONS, GENETIC;

BACILLUS SUBTILIS; BACILLUS THURINGIENSIS;

EID: 77955417593     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.06.021     Document Type: Article

References (27)

1. Adams L.F., Brown K.L., Whiteley H.R. Molecular cloning and characterization of two genes encoding sigma factors that direct transcription from a Bacillus thuringiensis crystal protein gene promoter. J. Bacteriol. 1991, 173:3846-3854.
2. Agaisse H., Lereclus D. Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA toxin gene is not dependent on a sporulation-specific sigma factor and is increased in a spo0A mutant. J. Bacteriol. 1994, 176:4734-4741.
3. Agaisse H., Lereclus D. Structural and functional analysis of the promoter region involved in full expression of the cryIIIA toxin gene of Bacillus thuringiensis. Mol. Microbiol. 1994, 13:97-107.
4. Agaisse H., Lereclus D. How does Bacillus thuringiensis produce so much insecticidal crystal protein?. J. Bacteriol. 1995, 177:6027-6032.
5. Agaisse H., Lereclus D. STAB-SD: a Shine-Dalgarno sequence in the 5' untranslated region is a determinant of mRNA stability. Mol. Microbiol. 1996, 20:633-643.
6. Bechhofer D.H., Dubnau D. Induced mRNA stability in Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 1987, 84:498-502.
7. Brown K.L., Whiteley H.R. Isolation of a Bacillus thuringiensis RNA polymerase capable of transcribing crystal protein genes. Proc. Natl. Acad. Sci. U.S.A. 1988, 85:4166-4170.
8. Brown K.L., Whiteley H.R. Isolation of the second Bacillus thuringiensis RNA polymerase that transcribes from a crystal protein gene promoter. J. Bacteriol. 1990, 172:6682-6688.
9. de Souza M.T., Lecadet M.M., Lereclus D. Full expression of the cryIIIA toxin gene of Bacillus thuringiensis requires a distant upstream DNA sequence affecting transcription. J. Bacteriol. 1993, 175:2952-2960.
10. Dervyn E., Poncet S., Klier A., Rapoport G. Transcriptional regulation of the cryIVD gene operon from Bacillus thuringiensis subsp. israelensis. J. Bacteriol. 1995, 177:2283-2291.
11. Glatz E., Nilsson R.P., Rutberg L., Rutberg B. A dual role for the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability. Mol. Microbiol. 1996, 19:319-328.
12. Guerout-Fleury A.M., Frandsen N., Stragier P. Plasmids for ectopic integration in Bacillus subtilis. Gene 1996, 180:57-61.
13. Haldenwang W.G. The sigma factors of Bacillus subtilis. Microbiol. Rev. 1995, 59:1-30.
14. Hambraeus G., Karhumaa K., Rutberg B. A 5' stem-loop and ribosome binding but not translation are important for the stability of Bacillus subtilis aprE leader mRNA. Microbiology 2002, 148:1795-1803.
15. Harwood C.R., Cutting S.M. Molecular Biological Methods for Bacillus 1990, John Wiley & Sons, Inc., New York, NY.
16. Helmann J.D. Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucleic Acids Res. 1995, 23:2351-2360.
17. Jan J., Valle F., Bolivar F., Merino E. Construction of protein overproducer strains in Bacillus subtilis by an integrative approach. Appl. Microbiol. Biotechnol. 2001, 55:69-75.
18. Jurgen B., Schweder T., Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol. Gen. Genet. 1998, 258:538-545.
19. Lereclus D., Agaisse H., Gominet M., Chaufaux J. Overproduction of encapsulated insecticidal crystal proteins in a Bacillus thuringiensis spo0A mutant. Biotechnology (NY) 1995, 13:67-71.
20. Mathy N., Benard L., Pellegrini O., Daou R., Wen T., Condon C. 5'-to-3' exoribonuclease activity in bacteria: role of RNase J1 in rRNA maturation and 5' stability of mRNA. Cell 2007, 129:681-692.
21. Miller J.H. Experiments in Molecular Genetics 1972, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
22. Ogura M., Matsuzawa A., Yoshikawa H., Tanaka T. Bacillus SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE. J. Bacteriol. 2004, 186:3056-3064.
23. Olmos J., Bolanos V., Causey S., Ferrari E., Bollvar F., Valle F. A functional Spo0A is required for maximal aprE expression in Bacillus subtilis. FEBS Lett. 1996, 381:29-31.
24. Park S.H., Koo B.T., Shin B.S., Choi S.K., Jeong Y.M., Pan J.G., Kim J.I. Characterization of 1925 Bacillus thuringiensis isolates from plants in Korea. Kor. J. Appl. Microbiol. Biotechnol. 1997, 25:159-165.
25. Schallmey M., Singh A., Ward O.P. Developments in the use of Bacillus species for industrial production. Can. J. Microbiol. 2004, 50:1-17.
26. Schumann W. Production of recombinant proteins in Bacillus subtilis. Adv. Appl. Microbiol. 2007, 62:137-189.
27. Westers L., Westers H., Quax W.J. Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim. Biophys. Acta 2004, 1694:299-310.