Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing

Nature Protocols

Volumn 11, Issue 5, 2016, Pages 853-871

  Hu, Jiazhi ^a   Meyers, Robin M ^a   Dong, Junchao ^a   Panchakshari, Rohit A ^a   Alt, Frederick W ^a,b   Frock, Richard L ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SINGLE STRANDED DNA; DEOXYRIBONUCLEASE;

ARTICLE; CELL HETEROGENEITY; DNA DAMAGE; DNA FRAGMENTATION; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; GENE AMPLIFICATION; GENE TRANSLOCATION; HIGH THROUGHPUT SEQUENCING; HUMAN; NEXT GENERATION SEQUENCING; OXIDATIVE STRESS; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; TRANSCRIPTION INITIATION SITE; TURNAROUND TIME; ANIMAL; BIOLOGY; CELL CULTURE; CHROMOSOME; GENE LIBRARY; GENETICS; GENOME; MAMMAL; MOUSE; POLYMERASE CHAIN REACTION; PROCEDURES;

ANIMALS; CELLS, CULTURED; CHROMOSOMES; COMPUTATIONAL BIOLOGY; DEOXYRIBONUCLEASES; DNA BREAKS, DOUBLE-STRANDED; DNA, SINGLE-STRANDED; GENE LIBRARY; GENOME; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MAMMALS; MICE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TRANSLOCATION, GENETIC;

EID: 84962784449     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.043     Document Type: Article

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