Characterization and Higher-Order Structure Assessment of an Interchain Cysteine-Based ADC: Impact of Drug Loading and Distribution on the Mechanism of Aggregation

Bioconjugate Chemistry

Volumn 27, Issue 3, 2016, Pages 604-615

  Guo, Jianxin ^a   Kumar, Sandeep ^a   Chipley, Mark ^a   Marcq, Olivier ^a,c   Gupta, Devansh ^b   Jin, Zhaowei ^a   Tomar, Dheeraj S ^a   Swabowski, Cecily ^a   Smith, Jacquelynn ^a   Starkey, Jason A ^a   Singh, Satish K ^a  

^a PFIZER INC   (United States)

^b Princeton University   (United States)

^c Agensys Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL STABILITY; HYDROPHOBICITY; ISOMERS; MOLECULAR MODELING; MONOCLONAL ANTIBODIES; SULFUR COMPOUNDS;

% REDUCTIONS; ANTIBODY-DRUG CONJUGATES; CHEMICAL DENATURATION; CYTOTOXIC; DRUG DISTRIBUTION; DRUG LOADING; HIGH-ORDER STRUCTURE; HIGHER-ORDER STRUCTURE; PHYSICAL STABILITY; STRUCTURE STABILITY;

AMINO ACIDS;

ANTIBODY CONJUGATE; CYSTEINE BASED ANTIBODY DRUG CONJUGATE; IMMUNOGLOBULIN G1 ANTIBODY; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG; CYSTEINE; DRUG;

ARTICLE; CALORIMETRY; DENATURATION; DRUG CONJUGATION; DRUG STRUCTURE; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR MODEL; PROTEIN AGGREGATION; PROTEIN QUATERNARY STRUCTURE; STORAGE TEMPERATURE; TEMPERATURE STRESS; THERMAL ANALYSIS; THERMOGRAPHY; CHEMICAL STRUCTURE; CHEMISTRY; DIFFERENTIAL SCANNING CALORIMETRY; SPECTROFLUOROMETRY;

CALORIMETRY, DIFFERENTIAL SCANNING; CHROMATOGRAPHY, LIQUID; CYSTEINE; IMMUNOCONJUGATES; MASS SPECTROMETRY; MOLECULAR STRUCTURE; PHARMACEUTICAL PREPARATIONS; SPECTROMETRY, FLUORESCENCE;

EID: 84961763048     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/acs.bioconjchem.5b00603     Document Type: Article

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