Domain-level stability of an antibody monitored by reduction, differential alkylation, and mass spectrometry analysis

Analytical Biochemistry

Volumn 400, Issue 2, 2010, Pages 244-250

  Liu, Hongcheng ^a   Chumsae, Chris ^a   Gaza Bulseco, Georgeen ^a   Goedken, Eric R ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

Alkylation; Antibody; Mass spectrometry

Indexed keywords

ALKYLATION; AMINO ACIDS; ANTIBODIES; COVALENT BONDS; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; SULFUR COMPOUNDS; THERMODYNAMIC STABILITY;

CYSTEINE RESIDUES; DENATURANT CONCENTRATION; GUANIDINE HYDROCHLORIDE; HUMAN IMMUNOGLOBULIN; INTERMEDIATE RESISTANCE; INTRINSIC TRYPTOPHAN FLUORESCENCES; MASS SPECTROMETRY ANALYSIS; RECOMBINANT MONOCLONAL ANTIBODY;

REDUCTION;

GUANIDINE; IMMUNOGLOBULIN G1 ANTIBODY; IODOACETIC ACID; MONOCLONAL ANTIBODY;

ALKYLATION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DENATURATION; DISULFIDE BOND; INCUBATION TIME; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN STABILITY; PURIFICATION; THERMODYNAMICS; THERMOSTABILITY;

EID: 77950689571     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.02.004     Document Type: Article

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