Conformational Disorganization within the Active Site of a Recently Evolved Organophosphate Hydrolase Limits Its Catalytic Efficiency

Biochemistry

Volumn 55, Issue 9, 2016, Pages 1408-1417

  Mabbitt, Peter D ^a   Correy, Galen J ^a   Meirelles, Tamara ^a   Fraser, Nicholas J ^a   Coote, Michelle L ^a,b   Jackson, Colin J ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b Australian Research Council s Centre of Excellence for Electromaterials Science   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DETOXIFICATION; ENZYMES; HYDROLASES; MOLECULAR BIOLOGY; MOLECULAR DYNAMICS;

CATALYTIC EFFICIENCIES; CATALYTIC MECHANISMS; ENZYMATIC ACTIVITIES; MOLECULAR DYNAMICS SIMULATIONS; NANOSECOND TIME SCALE; ORGANOPHOSPHATE HYDROLASE; ORGANOPHOSPHATE INSECTICIDES; X RAY CRYSTAL STRUCTURES;

CRYSTAL STRUCTURE;

HYDROLASE; ORGANOPHOSPHATE; PHOSPHATASE; PHOSPHORYLPHOSPHATASE;

ARTICLE; CATALYTIC EFFICIENCY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; DEPHOSPHORYLATION; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; HYDROGEN BOND; HYDROLYSIS; MICHAELIS CONSTANT; MOLECULAR DYNAMICS; MOLECULAR EVOLUTION; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTON TRANSPORT; STEADY STATE; ANIMAL; BINDING SITE; CHEMISTRY; INSECT; METABOLISM; PHYSIOLOGY; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

ANIMALS; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; INSECTS; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

EID: 84960374864     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.5b01322     Document Type: Article

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