Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search

ACS Chemical Biology

Volumn 10, Issue 2, 2015, Pages 611-621

  Porter, Joanne L ^a   Boon, Priscilla L S ^a   Murray, Tracy P ^a   Huber, Thomas ^a   Collyer, Charles A ^b   Ollis, David L ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID; ENZYME VARIANT; ESTER; HYDROLASE; 4-NITROPHENYL ACETATE; CARBOXYLESTERASE; CARBOXYMETHYLENEBUTENOLIDASE; MOLECULAR LIBRARY; NITROPHENOL;

ARTICLE; BIOTECHNOLOGY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; MOLECULAR EVOLUTION; MUTAGENESIS; MUTATION RATE; PRIORITY JOURNAL; THERMOSTABILITY; BIOENGINEERING; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; DIRECTED MOLECULAR EVOLUTION; GENETICS; KINETICS; METABOLISM; MOLECULAR LIBRARY; PROCEDURES; PROTEIN CONFORMATION; PROTEIN FOLDING;

BIOENGINEERING; CARBOXYLIC ESTER HYDROLASES; CRYSTALLIZATION; DIRECTED MOLECULAR EVOLUTION; KINETICS; MODELS, MOLECULAR; NITROPHENOLS; PROTEIN CONFORMATION; PROTEIN FOLDING; SMALL MOLECULE LIBRARIES; SUBSTRATE SPECIFICITY;

EID: 84923385007     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb500809f     Document Type: Article

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